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Proteomic Analysis of Mature Lagenaria siceraria Seed

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Abstract

Lagenaria siceraria (bottle gourd) class belongs to Magnoliopsida family curcurbitaceae that is a traditionally used medicinal plant. Fruit of this plant are widely used as a therapeutic vegetable in various diseases, all over the Asia and Africa. Various parts of this plant like fruit, seed, leaf and root are used as alternative medicine. In the present study, primarily, we have focused on proteomic analysis of L. siceraria seed using phenol extraction method for protein isolation. Twenty-four colloidal coomassie blue stained protein spots were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS) after resolving on two-dimensional gel electrophoresis. Out of 24 identified protein spots, four were grouped as unidentified proteins which clearly suggest that less work has been done in the direction of plant seed proteomics. These proteins have been found to implicate in various functions such as biosynthesis of plant cell wall polysaccharides and glycoproteins, serine/threonine kinase activity, plant disease resistance and transferase activity against insects by means of insecticidal and larval growth inhibitory, anti-HIV, antihelmintic and antimicrobial properties. By Blast2GO annotation analysis, amongst the identified proteins of L. siceraria, molecular function for majority of proteins has indispensable role in catalytic activity, few in binding activity and antioxidant activity; it is mostly distributed in cell, organelle, membrane and macromolecular complex. Most of them involved in biological process such as metabolic process, cellular process, response to stimulus, single organism process, signalling, biological recognition, cellular component organization or biogenesis and localization.

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Acknowledgments

This work was supported by Women Scientists Scheme (WOS-A), Department of Science and Technology (DST), India. Neha Kumari and Md. Tajmul thank the DST, India and the Indian Council of Medical Research (ICMR), India for their fellowship, respectively.

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Authors and Affiliations

  1. Department of Biophysics, All India Institute of Medical Sciences, New Delhi, 110029, India

    Neha Kumari, Md. Tajmul & Savita Yadav

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  1. Neha Kumari
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  2. Md. Tajmul
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  3. Savita Yadav
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Correspondence to Savita Yadav.

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Kumari, N., Tajmul, M. & Yadav, S. Proteomic Analysis of Mature Lagenaria siceraria Seed. Appl Biochem Biotechnol 175, 3643–3656 (2015). https://doi.org/10.1007/s12010-015-1532-3

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  • DOI: https://doi.org/10.1007/s12010-015-1532-3

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