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Genetic Diversity and Some Aspects of Antimicrobial Activity of Lactic Acid Bacteria Isolated from Goat Milk

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Abstract

Lactic acid bacteria (LAB, n = 57) were previously obtained from raw goat milk, identified as Lactococcus spp. (n = 24) and Enterococcus spp. (n = 33), and characterized as bacteriocinogenic. Fingerprinting by pulsed field gel electrophoresis (PFGE) demonstrated high genetic diversity, and 30 strains were selected and exhibited strong antimicrobial activity against 46 target strains (LAB, spoilage, and foodborne pathogens). Six strains (Lactococcus lactis: GLc03 and GLc05; and Enterococcus durans: GEn09, GEn12, GEn14, and GEn17) were selected to characterize their bacteriocinogenic features, using Listeria monocytogenes ATCC 7644 as the target. The six strains produced bacteriocins at higher titer when incubated in MRS at 37 °C up to 12 h, when compared to growth at 25 and 30 °C. The produced bacteriocins kept their antimicrobial activity after exposure to 100 °C for 2 h and 121 °C for 20 min; the antimicrobial activity was also observed after treatment at pH 2.0 to 10.0, except for GLc03. L. monocytogenes populations were reduced approximately two logs after treatment with cell-free supernatants from the selected strains. These data show that goat milk can contain a diverse microbiota able to inhibit L. monocytogenes, a common pathogen found in dairy products, and can be potentially employed in biopreservation of food produced under different processing conditions.

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Acknowledgments

The authors are thankful to CNPq, CAPES, FAPESP, and FAPEMIG.

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  1. Departamento de Veterinária, Universidade Federal de Viçosa, Campus UFV, 36570 900, Viçosa, MG, Brazil

    Valéria Quintana Cavicchioli, Wesley dos Santos Dornellas, Luana Martins Perin, Fábio Alessandro Pieri, Svetoslav Dimitrov Todorov & Luís Augusto Nero

  2. Departamento de Ciências Básicas da Saúde, Universidade Federal de Juiz de Fora, Campus Governador Valadares, 35020 220, Governador Valadares, MG, Brazil

    Fábio Alessandro Pieri

  3. Faculdade de Ciências Farmacêuticas, Departamento de Alimentos e Nutrição Experimental, Universidade de São Paulo, Av. Prof. Lineu Prestes 580, 05508 000, São Paulo, SP, Brazil

    Bernadette Dora Gombossy de Melo Franco & Svetoslav Dimitrov Todorov

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  1. Valéria Quintana Cavicchioli
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  2. Wesley dos Santos Dornellas
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Cavicchioli, V.Q., Dornellas, W.d.S., Perin, L.M. et al. Genetic Diversity and Some Aspects of Antimicrobial Activity of Lactic Acid Bacteria Isolated from Goat Milk. Appl Biochem Biotechnol 175, 2806–2822 (2015). https://doi.org/10.1007/s12010-015-1511-8

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