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Protective Effect of Punica granatum Peel and Vitis vinifera Seeds on DEN-Induced Oxidative Stress and Hepatocellular Damage in Rats

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Abstract

This study was designed to find out the efficacy of ethanol extracts of Punica granatum peel and Vitis vinifera seeds on diethylnitrosamine (DEN)-induced oxidative stress and hepatocellular damage in Wistar rats. Rats were divided into four groups. The first group served as normal control, and the second group received DEN at a dose of 200 mg/kg body weight by single intraperitoneal administration. The third one received DEN as in DEN-treated group and co-treated with 400 mg/kg P. granatum peel extract. The final group also received DEN and co-treated with 400 mg/kg V. vinifera seed extract. DEN administration to rats resulted in significantly elevated levels of serum SGPT, SGOT, ALP, and GGT which is indicative of hepatocellular damage. DEN-induced oxidative stress was confirmed by elevated levels of lipid peroxides and decreased activities of superoxide dismutase, catalase, and glutathione peroxidase in the serum and liver tissues. The status of non-enzymatic antioxidants like vitamin C, vitamin E, and reduced glutathione were also found to be decreased in serum and tissues of DEN-administered rats. Co-treatment with the P. granatum peel and V. vinifera seed extracts orally for 12 weeks significantly reversed the DEN-induced alterations in the serum and liver tissues.

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Authors and Affiliations

  1. Department of Biotechnology, Arulmigu Meenakshi Amman College of Engineering, Vadamavandal, 604 410, Thiruvanamalai District, Tamil Nadu, India

    Ashok K. Kumar

  2. Department of Biochemistry, Bharathi Women’s College, 600 108, Chennai, Tamil Nadu, India

    Vijayalakshmi K

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  1. Ashok K. Kumar
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  2. Vijayalakshmi K
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Correspondence to Ashok K. Kumar.

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Kumar, A.K., K, V. Protective Effect of Punica granatum Peel and Vitis vinifera Seeds on DEN-Induced Oxidative Stress and Hepatocellular Damage in Rats. Appl Biochem Biotechnol 175, 410–420 (2015). https://doi.org/10.1007/s12010-014-1276-5

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