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Improved Pretreatment Process Using an Electron Beam for Optimization of Glucose Yield with High Selectivity

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Abstract

In this study, electron beam irradiation (EBI) assisted by a dilute acid pretreatment process was investigated to improve the glucose yield and show high selectivity in the enzymatic hydrolysis of rice straw. In the first step, EBI of rice straw was performed at various doses ranging from 50 to 500 kGy. The electron beam-irradiated rice straw was then autoclaved with 3 % dilute acid at 120 °C for 1 h. The pretreated rice straw was finally subjected to enzymatic hydrolysis at 50 °C for 24, 48, and 72 h by 70 filter paper units (FPU)/mL cellulase and 40 cellobiose units (CbU)/mL glucosidase. Glucose was obtained with a very high selectivity of 92.7 % and a total sugar yield of 80 % from pretreated rice straw after 72 h of enzymatic hydrolysis.

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Acknowledgment

This study was supported by the Nuclear R&D program of the Korea Science and Engineering Foundation, which is funded by the Ministry of Science, ICT and Future Planning of the Republic of Korea.

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Authors and Affiliations

  1. Research Division for Industry & Environment, Korea Atomic Energy Research Institute, 29 Geumgu-gil, Jeongeup-si, Jeollabuk-do, 580-185, Korea

    Byoung-Min Lee, Jin-Young Lee, Phil-Hyun Kang & Joon-Pyo Jeun

  2. Department of Polymer Science and Engineering, Chungnam National University, 99 Daehangno, Yuseong-gu, Daejeon, 305-764, Korea

    Sung-Kwon Hong

Authors
  1. Byoung-Min Lee
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  2. Jin-Young Lee
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  3. Phil-Hyun Kang
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  4. Sung-Kwon Hong
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  5. Joon-Pyo Jeun
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Correspondence to Joon-Pyo Jeun.

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Highlights

1. A facile two-step method was developed for producing fermentable sugars.

2. The synergic effect of dilute acid pretreatment on the EBI of rice straw was assessed.

3. A 75 % yield and 92.7 % selectivity were obtained for glucose.

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Lee, BM., Lee, JY., Kang, PH. et al. Improved Pretreatment Process Using an Electron Beam for Optimization of Glucose Yield with High Selectivity. Appl Biochem Biotechnol 174, 1548–1557 (2014). https://doi.org/10.1007/s12010-014-1138-1

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  • DOI: https://doi.org/10.1007/s12010-014-1138-1

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