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Antibacterial Activity of Bacillus Lipopeptides Vehiculized and Delivered by Biopolymeric Films

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Abstract

Active films based on different biopolymers (pectin, Brea gum, carboxymethylcellulose, and composite starch-gelatin) were prepared by casting. Lipopeptides synthesized by bacteria of the Bacillus genus were added as antimicrobial active agent. Antibacterial activity was evaluated and quantified by direct contact technique against different strains of Listeria monocytogenes, Escherichia coli, and Salmonella enterica sp. Incorporation of lipopeptides did not cause significant changes in mechanical and barrier properties of films. Antibacterial effect of functionalized films depended on the nature of the polymeric matrix and the pathogen studied. Functionalized pectin films inhibited the growth of all strains studied, with non-functionalized pectin films also showing inhibition of L. monocytogenes. Functionalized starch-gelatin films only inhibited the growth of L. monocytogenes. The other matrices did not show adequate performance for this type of application. The results suggested that pectin and starch-gelatin composite can be used as effective lipopeptide carriers to produce antimicrobial active films for food preservation.

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All data generated or analyzed during this study are included in this published article.

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Acknowledgements

The authors thank the staff of Applied Bacteriology Laboratory (INIQUI, CONICET, UNSa).

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The financial support provided by the Consejo de Investigación de la Universidad Nacional de Salta (CIUNSa-Proyecto 2472) y MINCyT is gratefully acknowledged.

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  1. Instituto de Investigaciones Para La Industria Química (INIQUI), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Salta. Av, Bolivia 5150, 4400, Salta, Argentina

    Colodro M. Verónica, Torres M. Julia, Slavutsky Aníbal M., Audisio M. Carina & Bertuzzi M. Alejandra

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  3. Slavutsky Aníbal M.
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All authors contributed to conception and design of the study. Material preparation, data collection, and analysis were performed by Maria Veronica Colodro and Maria Julia Torres. The first draft of the manuscript was written by Maria Veronica Colodro, and all authors commented and contributed to subsequent versions of the manuscript. All authors read and approved the final manuscript.

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Verónica, C.M., Julia, T.M., M., S.A. et al. Antibacterial Activity of Bacillus Lipopeptides Vehiculized and Delivered by Biopolymeric Films. Food Bioprocess Technol 17, 504–515 (2024). https://doi.org/10.1007/s11947-023-03139-5

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