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Microwave-Intensified Enzymatic Deproteinization of Australian Rock Lobster Shells (Jasus edwardsii) for the Efficient Recovery of Protein Hydrolysate as Food Functional Nutrients

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Abstract

Enzymatic deproteinization of lobster shells is an important step in developing a novel biorefinery process for the recovery of both protein and chitin. This study aimed to develop an efficient enzymatic deproteinization of lobster shells for protein recovery while providing the residual fraction suitable for further chitin recovery. In comparison with conventional incubation, the microwave-intensified enzymatic deproteinization (MIED) of Australian rock lobster shells significantly improved the deproteinization degree from 58 to 85.8 % and reduced the residual protein content from 96.4 to 65.4 mg/g, respectively. The protein hydrolysate produced by MIED had excellent functionality (solubility 91.7 %, water absorption 32 %, oil absorption 2.3 mL/g, foaming 51.3 %, emulsification 91.3 %) and high nutritional quality (34 % essential amino acids, 45.4 mg/g arginine, lysine/arginine ratio 0.69) with potential applications for food industry. With the considerably low residual protein, the MIEDs are suitable for further chitin recovery.

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Acknowledgments

The authors wish to acknowledge the Australian government for offering Trung T. Nguyen a PhD scholarship, the South Australian Government and Ferguson Australia Pty Ltd for the IVP funding support from the Premier’s Research and Industry Fund, as well as the Centre for Marine Bio-products Development, Flinders University.

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Authors and Affiliations

  1. Centre for Marine Bio-products Development, Flinders University, Adelaide, Australia

    Trung T. Nguyen, Wei Zhang, Andrew R. Barber, Peng Su & Shan He

  2. Department of Medical Biotechnology, School of Medicine, Flinders University, Adelaide, Australia

    Trung T. Nguyen, Wei Zhang, Andrew R. Barber, Peng Su & Shan He

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  1. Trung T. Nguyen
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  2. Wei Zhang
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Correspondence to Wei Zhang.

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Nguyen, T.T., Zhang, W., Barber, A.R. et al. Microwave-Intensified Enzymatic Deproteinization of Australian Rock Lobster Shells (Jasus edwardsii) for the Efficient Recovery of Protein Hydrolysate as Food Functional Nutrients. Food Bioprocess Technol 9, 628–636 (2016). https://doi.org/10.1007/s11947-015-1657-y

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  • DOI: https://doi.org/10.1007/s11947-015-1657-y

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