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Philadelphia-Negative Myeloproliferative Neoplasms: Laboratory Workup in the Era of Next-Generation Sequencing

  • Molecular Testing and Diagnostics (J Khoury, Section Editor)
  • Published:
Current Hematologic Malignancy Reports Aims and scope Submit manuscript

Abstract

Purpose of Review

To review the impact of next-generation sequencing (NGS) on laboratory approach of myeloproliferative neoplasms (MPNs).

Recent Findings

Next-generation sequencing has provided valuable information on the mutational landscape of MPNs and has been used for various applications, including diagnosis, risk stratification, monitoring of residual disease or disease progression, and target therapy. Most commonly, targeted sequencing of a panel of genes that have been shown to be recurrently mutated in myeloid neoplasms is used. Although numerous studies have shown the benefit of using NGS in the routine clinical care of MPN patients, the complexity of NGS data and how these data may contribute to the clinical outcome have limited the development of a standard clinical guideline.

Summary

We review recent literature and discuss how to interpret and use NGS data in the clinical care of MPN patients.

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Papers of particular interest, published recently, have been highlighted as: • Of importance •• Of major importance

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  1. Department of Hematopathology, University of Texas MD Anderson Cancer Center, Houston, TX, USA

    Zhuang Zuo, Shaoying Li, Jie Xu, M. James You, Joseph D. Khoury & C. Cameron Yin

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  1. Zhuang Zuo
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  2. Shaoying Li
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Zuo, Z., Li, S., Xu, J. et al. Philadelphia-Negative Myeloproliferative Neoplasms: Laboratory Workup in the Era of Next-Generation Sequencing. Curr Hematol Malig Rep 14, 376–385 (2019). https://doi.org/10.1007/s11899-019-00534-8

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