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Air Sampling and Analysis of Aeroallergens: Current and Future Approaches

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Abstract

Purpose of Review

To review current air sampling instruments and analysis methods and to describe new approaches being developed.

Recent Findings

Spore trap sampling with analysis by microscopy remains the most widely used methods for aeroallergen determination even though there are often long delays from sample acquisition to data availability, as well as a need for specially-trained staff for sample analysis. The use of immunoassays and molecular biology to analyze outdoor or indoor samples has expanded in recent years and has provided valuable data on allergen exposure. New automated sampling devices capture pollen, analyze, and identify pollen grains by light scattering, laser-induced fluorescence, microscopy, or holography using signal or image processing to classify the pollen in real time or near real time.

Summary

Air sampling data from current methods provide valuable information on aeroallergen exposure. The automated devices in use and under development show great potential but are not ready to replace existing aeroallergen networks.

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Authors and Affiliations

  1. Department of Biological Science, University of Tulsa, 74104, Tulsa, OK, USA

    Estelle Levetin

  2. Department of Biological Sciences, LeTourneau University, 75602, Longview, TX, USA

    Josh D. McLoud

  3. OSHTECH Incorporated, N6B 2M4, London, ON, Canada

    Peter Pityn

  4. Division of Allergy/Immunology, University of Nebraska Medical Center, 68198, Omaha, NE, USA

    Andrew C. Rorie

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Levetin, E., McLoud, J.D., Pityn, P. et al. Air Sampling and Analysis of Aeroallergens: Current and Future Approaches. Curr Allergy Asthma Rep 23, 223–236 (2023). https://doi.org/10.1007/s11882-023-01073-2

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