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Behavioral responses of Hyalesthes obsoletus to host-plant volatiles cues

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Abstract

The polyphagous planthopper Hyalesthes obsoletus Signoret is considered to be the principal vector of stolbur phytoplasma, which is associated with yellow diseases of grapevine. To explore the possibility of developing novel control strategies, the behavioral responses to six synthetic mixtures and nine single compounds, previously identified from the headspace of Vitex agnus-castus L. (chaste tree) and Urtica dioica L. (nettle), were investigated in Y-tube bioassays. Choice tests revealed differences in the behavioral responses of males and females to the volatiles that they were exposed to. Males were attracted to a mixture containing (E)-β-caryophyllene, 1,8-cineole, (E,E)-α-farnesene, (E)-β-farnesene, and methyl salicylate (mixture 2). The addition of methyl benzoate to this five-compound mixture (mixture 3) did not attract males but elicited positive responses in females. Furthermore, females were attracted to a mixture containing (E)-β-caryophyllene, (E,E)-α-farnesene, (Z)-3-hexenyl acetate, (Z)-3-hexen-1-ol, and benzothiazole (mixture 4), but here addition of methyl salicylate (mixture 5) did not attract females. Neither males nor females showed attractivity or repellency toward the singly tested compounds. This study enhances knowledge on the interaction of insect behaviorally effective constituents in complex plant volatile mixtures. The attractive mixtures of plant volatiles identified suggest the possibility of using them in monitoring and management of H. obsoletus.

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Acknowledgements

This study was funded by the Italian Ministry of Education, Universities and Research (MIUR), Research Project of National Interest (PRIN) ‘Mechanisms of host-plant location and host-plant preferences in two leafhoppers (Hemiptera: Auchenorrhyncha), vectors of grapevine yellow diseases’. Comments and suggestions made by three anonymous reviewers have helped us to improve the manuscript.

Authors’ contribution

PR and RLM conceived and designed the experiments. PR, RLM, and EP performed the experiments. PR, RLM, EP, and NI analyzed the results. PR contributed reagents/materials/analysis tools. PR, RLM, EP, and NI wrote the manuscript.

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Authors and Affiliations

  1. Dipartimento di Scienze Agrarie, Alimentari ed Ambientali, Università Politecnica delle Marche, 60131, Ancona, Italy

    Paola Riolo, Roxana L. Minuz & Nunzio Isidoro

  2. Dipartimento di Scienze Agrarie e Forestali, Università degli Studi di Palermo, 90128, Palermo, Italy

    Ezio Peri

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  1. Paola Riolo
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  2. Roxana L. Minuz
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  3. Ezio Peri
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  4. Nunzio Isidoro
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Correspondence to Paola Riolo.

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All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Handling Editor: Jarmo Holopainen.

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Riolo, P., Minuz, R.L., Peri, E. et al. Behavioral responses of Hyalesthes obsoletus to host-plant volatiles cues. Arthropod-Plant Interactions 11, 71–78 (2017). https://doi.org/10.1007/s11829-016-9467-3

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  • DOI: https://doi.org/10.1007/s11829-016-9467-3

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