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Expression of the sweet potato peroxidase gene swpa4 in Arabidopsis activates defense genes mediated by reactive oxygen species and nitric oxide

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Abstract

Previously transgenic tobacco plants over-expressing the sweet potato peroxidase gene swpa4 exhibited increased tolerance to various stress conditions. Expression of swpa4 in tobacco also has a positive effect on expression of genes related to reactive oxygen species (ROS) and nitric oxide (NO). Expression levels of respiratory burst oxidase protein D (RBOHD), a major gene regulating ROS, and nitric oxide associated 1 (NOA1), a gene involved in NO generation, are significantly affected by peroxidase inhibitor treatments. In this study, swpa4 was expressed in the rbohD and noa1 knock-out mutants of Arabidopsis, as well as in wild-type plants. The transgenic plants showed increased levels of ROS and NO. Expression of swpa4 activated expression of genes related to ROS and NO. This suggested that swpa4 regulated H2O2 and NO homeostasis, thereby establishing a potential molecular link between the defense signaling pathways mediated by ROS and NO.

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Abbreviations

APX:

Ascorbate peroxidase

CAT:

Catalase

NOA:

Nitric oxide associated

RBOH:

Respiratory burst oxidase protein

RNS:

Reactive nitrogen species

ROS:

Reactive oxygen species

POD:

Peroxidase

PR:

Pathogenesis-related

SOD:

Superoxide dismutase

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Acknowledgements

This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning (NRF-2018R1A1A1A05018446), and the KRIBB Initiative Program.

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Authors and Affiliations

  1. Department of Biology Education, Gyeongsang National University, Jinju, 660-701, South Korea

    Yun-Hee Kim

  2. Laboratory of Plant Functional Genomics, School of Applied Biosciences, Kyungpook National University, Daegu, South Korea

    Byung-Wook Yun

  3. Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwakak-ro, Yuseong-gu, Daejeon, 305-806, South Korea

    Sang-Soo Kwak

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  1. Yun-Hee Kim
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  2. Byung-Wook Yun
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  3. Sang-Soo Kwak
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Correspondence to Yun-Hee Kim.

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Kim, YH., Yun, BW. & Kwak, SS. Expression of the sweet potato peroxidase gene swpa4 in Arabidopsis activates defense genes mediated by reactive oxygen species and nitric oxide. Plant Biotechnol Rep 13, 329–336 (2019). https://doi.org/10.1007/s11816-019-00546-z

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