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Enhanced D-ribose production by genetic modification and medium optimization in Bacillus subtilis 168

  • Biotechnology
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Abstract

D-ribose, a five-carbon sugar with diverse applications, is mainly produced by transketolase(tkt)-deficient Bacillus subtilis (B. Subtilis). We constructed B.subtilis SFR-3A by replacing the corresponding sites of B. subtilis 168 with the mutation site of tkt in the “wild” D-ribose high-producing strain B. subtilis SFR-4, resulting in 5.29 g/L of D-ribose. In the meantime, B.subtilis SFR-159 was constructed by completely removing the tkt gene of B. subtilis 168 with a higher production of 6.21 g/L. Through medium optimization, batch fermentation of SFR-3A and SFR-159 gave the best result of 27.56 g/L and 29.89 g/L, which corresponds to productivity of 0.51 g/L/h and 0.41 g/L/h, respectively. In this work, SFR-3A showed more latent capacity over SFR-159 as to productivity and had greater potential to serve as a platform for D-ribose production.

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Authors and Affiliations

  1. Key Laboratory of Food and Fermentation Engineering of Shandong Province, Shandong Food Ferment Industry Research & Design Institute, Jinan, 250013, P. R. China

    Chen Zhao, Xiang-Ying Zhao, Jian-Jun Liu, Jun-Jiao Zhang, Jia-Xiang Zhang & Li-He Zhang

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  1. Chen Zhao
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  2. Xiang-Ying Zhao
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  3. Jian-Jun Liu
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  4. Jun-Jiao Zhang
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  6. Li-He Zhang
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Correspondence to Xiang-Ying Zhao.

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Zhao, C., Zhao, XY., Liu, JJ. et al. Enhanced D-ribose production by genetic modification and medium optimization in Bacillus subtilis 168. Korean J. Chem. Eng. 35, 1137–1143 (2018). https://doi.org/10.1007/s11814-017-0356-y

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  • DOI: https://doi.org/10.1007/s11814-017-0356-y

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