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Integrated fabrication-conjugation approaches for biomolecular assembly and protein sensing with hybrid microparticle platforms and biofabrication - A focused minireview

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Abstract

Controlled manufacturing of polymeric hydrogel microparticles is crucial, yet challenging, for rapid and sensitive detection of biomacromolecules in biodiagnostics and biosensing applications. Our approach is an integrated fabrication-conjugation strategy utilizing a simple and robust micromolding technique and biofabrication with a potent aminopolysaccharide chitosan as an efficient conjugation handle for high-yield bioorthogonal conjugation reactions. We present a concise overview of our recent findings in the controlled fabrication of shape-encoded or core-shell structured microparticles consisting of poly(ethylene glycol) (PEG) and short single-stranded (ss) DNA or chitosan, and their utility in the covalent conjugation and nucleic acid hybridization-based assembly of target ssDNAs, proteins and viral nanotemplates. Particularly, two novel routes to achieve substantially improved protein conjugation capacity and kinetics are presented from our recent reports: tobacco mosaic virus (TMV) as a high capacity nanotubular template and polymerization-induced phase separation (PIPS) of pre-polymer droplets for controlled core-shell structure formation. We envision that our fabrication-conjugation approaches reported here, combined with our current and future endeavors in improved fabrication and design of controlled structures with chemical functionalities, should permit a range of manufacturing strategies for advanced functional microscale materials and platforms in a wide array of applications.

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Authors and Affiliations

  1. Department of Chemical and Biological Engineering, Tufts University, 4 Colby St., Medford, MA, 02155, USA

    Sukwon Jung & Hyunmin Yi

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  1. Sukwon Jung
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  2. Hyunmin Yi
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Correspondence to Hyunmin Yi.

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Hyunmin Yi is currently an Associate Professor at the Department of Chemical and Biological Engineering of Tufts University in Massachusetts, U.S.A. He received his B.S. degree in Chemical Technology (Seoul National University), M.S. degree in Biochemical Engineering (Seoul National University) and Ph.D. degree in Chemical Engineering (University of Maryland at College Park), and was a postdoctoral fellow at the Center for Biosystems Research then an Assistant Research Scientist at the Department of Materials Science and Engineering, both at the University of Maryland at College Park, prior to arrival at Tufts in 2006. His research interests span broad areas of smart biomaterials, biofabrication, nanobiotechnology and biochemical engineering. Professor Yi was the recipient of KIChE President Award in 2013.

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Jung, S., Yi, H. Integrated fabrication-conjugation approaches for biomolecular assembly and protein sensing with hybrid microparticle platforms and biofabrication - A focused minireview. Korean J. Chem. Eng. 32, 1713–1719 (2015). https://doi.org/10.1007/s11814-015-0147-2

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  • DOI: https://doi.org/10.1007/s11814-015-0147-2

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