Abstract
As a novel food quality monitoring technology, intelligent freshness indicator has received wide attention in recent years. However, its poor safety and stability are the main problems hindering its practical application. Hence a new pH-sensing indicator based on bromocresol green (BCG) was developed in this study for nondestructive and real-time monitoring the freshness of marine fishes. The indicator was designed with a three-layer structure, using the polytetrafluoroethylene (PTFE) membrane with high hydrophobicity and air permeability as the inner layer to isolate the moisture in the package, BCG-coated filter paper as the color-changing layer to indicate the freshness of fish, and a transparent unidirectional permeable (TUP) membrane with moisture resistance as the out layer to isolate the moisture in the environment. This contributed to weaken the influence of humidity and prevent dye migration, so as to improve the accuracy and safety of the indicator. Therefore, a highly sensitive and distinguished color variation response to trimethylamine (TMA) standard solution with different concentrations was observed on the indicator. Additionally, the indicator showed a high color stability at different storage temperatures up to 14 days with total color differences (ΔE) less than 5.0. The indicator presented visible color variations from yellow to green then eventually to blue when applied to monitor the freshness of sea bass and salmon stored at 4°C, implying that fish was spoiled. Meanwhile, indicators ΔE value was significantly positively correlated with total volatile basic nitrogen (TVB-N) and total viable count (TVC) in sea bass and salmon samples. Thus, the pH-sensing indicator can be applied as a cost-effective and promising intelligent indicator for monitoring fish freshness.
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References
Argyri, A. A., Jarvis, R. M., Wedge, D., Xu, Y., Panagou, E. Z., Goodacre, R., et al., 2013. A comparison of Raman and FT-IR spectroscopy for the prediction of meat spoilage. Food Control, 29(2): 461–470, DOI: https://doi.org/10.1016/j.foodcont.2012.05.040.
Byrne, L., Lau, K. T., and Diamond, D., 2002. Monitoring of head-space total volatile basic nitrogen from selected fish species using reflectance spectroscopic measurements of pH sensitive films. Analyst, 127(10): 1338–1341, DOI: https://doi.org/10.1039/b206149j.
Chen, H., Wu, Y., Chen, Z., Jia, Y., Han, P., and Cheng, C., 2019. Effect of pullulan hydrolysates on the quality of Nile tilapia (Oreochromis niloticus) fillets during ice storage. Journal of Food Processing and Preservation, 43(8): e14043, DOI: https://doi.org/10.1111/jfpp.14043.
Chen, H. Z., Zhang, M., Bhandari, B., and Guo, Z., 2018. Applicability of a colorimetric indicator label for monitoring freshness of fresh-cut green bell pepper. Postharvest Biology and Technology, 140: 85–92, DOI: https://doi.org/10.1016/j.postharvbio.2018.02.011.
Chen, H. Z., Zhang, M., Bhandari, B., and Yang, C. H., 2019. Development of a novel colorimetric food package label for monitoring lean pork freshness. LWT-Food Science and Technology, 99: 43–49, DOI: https://doi.org/10.1016/j.lwt.2018.09.048.
Chen, H. Z., Zhang, M., Bhandari, B., and Yang, C. H., 2020. Novel pH-sensitive films containing curcumin and anthocyanins to monitor fish freshness. Food Hydrocolloids, 100: 105438, DOI: https://doi.org/10.1016/j.foodhyd.2019.105438.
Chun, H. N., Kim, B., and Shin, H. S., 2014. Evaluation of a freshness indicator for quality of fish products during storage. Food Science and Biotechnology, 23(5): 1719–1725, DOI: https://doi.org/10.1007/s10068-014-0235-9.
Efremenko, Y., and Mirsky, V. M., 2017. Virtual sensor array consisting of a single sensor element with variable affinity: An application for analysis of fish freshness. Sensors and Actuators B: Chemical, 241: 652–657, DOI: https://doi.org/10.1016/j.snb.2016.10.126.
Ezati, P., Priyadarshi, R., Bang, Y. J., and Rhim, J. W., 2021. CMC and CNF-based intelligent pH-responsive color indicator films integrated with shikonin to monitor fish freshness. Food Control, 126: 108046, DOI: https://doi.org/10.1016/j.foodcont.2021.108046.
Ezati, P., Tajik, H., and Moradi, M., 2019. Fabrication and characterization of alizarin colorimetric indicator based on cellulose-chitosan to monitor the freshness of minced beef. Sensors and Actuators B: Chemical, 285: 519–528, DOI: https://doi.org/10.1016/j.snb.2019.01.089.
Ezati, P., Tajik, H., Moradi, M., and Molaei, R., 2019. Intelligent pH-sensitive indicator based on starch-cellulose and alizarin dye to track freshness of rainbow trout fillet. International Journal of Biological Macromolecules, 132: 157–165, DOI: https://doi.org/10.1016/j.ijbiomac.2019.03.173.
Freitas, P. A. V., Silva, R. R. A., de Oliveira, T. V., Soares, R. R. A., Junior, N. S., Moraes, A. R. F., et al., 2020. Development and characterization of intelligent cellulose acetate-based films using red cabbage extract for visual detection of volatile bases. LWT-FoodScience and Technology, 132: 109780, DOI: https://doi.org/10.1016/j.lwt.2020.109780.
Gholampour, S., Jalali, H., Zhiani, R., Rashidi, H., and Motavalizadehkakhky, A., 2021. Biogenic amines to tune the LSPR adsorption peak of gold NPs for intelligent packaging application. Inorganic Chemistry Communications, 123: 108334, DOI: https://doi.org/10.1016/j.inoche.2020.108334.
Hassoun, A., and Karoui, R., 2015. Front-face fluorescence spectroscopy coupled with chemometric tools for monitoring fish freshness stored under different refrigerated conditions. Food Control, 54: 240–249, DOI: https://doi.org/10.1016/j.foodcont.2015.01.042.
Jia, Z., Shi, C., Zhang, J., and Ji, Z., 2021. Comparison of freshness prediction method for salmon fillet during different storage temperatures. Journal of the Science of Food and Agriculture, 101(12): 4987–4994, DOI: https://doi.org/10.1002/jsfa.11142.
Lee, E. J., and Shin, H. S., 2019. Development of a freshness indicator for monitoring the quality of beef during storage. Food Science and Biotechnology, 28(6): 1899–1906, DOI: https://doi.org/10.1007/s10068-019-00633-5.
Lee, K., Baek, S., Kim, D., and Seo, J., 2019. A freshness indicator for monitoring chicken-breast spoilage using a Tyvek® sheet and RGB color analysis. Food Packaging and Shelf Life, 19: 40–46, DOI: https://doi.org/10.1016/j.fpsl.2018.11.016.
Lee, K., Park, H., Baek, S., Han, S., Kim, D., Chung, S., et al., 2019. Colorimetric array freshness indicator and digital color processing for monitoring the freshness of packaged chicken breast. Food Packaging and Shelf Life, 22: 100408, DOI: https://doi.org/10.1016/j.fpsl.2019.100408.
Li, C., Hao, J., and Wu, K., 2019. Triethylamine-controlled Cu-BTC frameworks for electrochemical sensing fish freshness. Analytica Chimica Acta, 1085: 68–74, DOI: https://doi.org/10.1016/j.aca.2019.07.064.
Liu, D., Cui, Z., Shang, M., and Zhong, Y., 2021. A colorimetric film based on polyvinyl alcohol/sodium carboxymethyl cellulose incorporated with red cabbage anthocyanin for monitoring pork freshness. Food Packaging and Shelf Life, 28: 100641, DOI: https://doi.org/10.1016/j.fpsl.2021.100641.
Liu, J., Lan, W., Sun, X., and Xie, J., 2020. Effects of chitosan grafted phenolic acid coating on microbiological, physicochemical and protein changes of sea bass (Lateolabrax japonicus) during refrigerated storage. Journal of Food Science, 85(8): 2506–2515, DOI: https://doi.org/10.1111/1750-3841.15329.
Liu, X., Chen, K., Wang, J., Wang, Y., Tang, Y., Gao, X., et al., 2020. An on-package colorimetric sensing label based on a solgel matrix for fish freshness monitoring. Food Chemistry, 307: 125580, DOI: https://doi.org/10.1016/j.foodchem.2019.125580.
Liu, Z., Huang, M., Zhu, Q., Qin, J., and Kim, M. S., 2021. Nondestructive freshness evaluation of intact prawns (Fenneropenaeus chinensis) using line-scan spatially offset Raman spectroscopy. Food Control, 126: 108054, DOI: https://doi.org/10.1016/j.foodcont.2021.108054.
Majdinasab, M., Hosseini, S. M. H., Sepidname, M., Negahdarifar, M., and Li, P., 2018. Development of a novel colorimetric sensor based on alginate beads for monitoring rainbow trout spoilage. Journal of Food Science and Technology, 55(5): 1695–1704, DOI: https://doi.org/10.1007/s13197-018-3082-5.
Mo, R., Quan, Q., Li, T., Yuan, Q., Su, T., Yan, X., et al., 2017. An intelligent label for freshness of fish based on a porous anodic aluminum membrane and bromocresol green. Chemistry-Select, 2(28): 8779–8784, DOI: https://doi.org/10.1002/slct.201701313.
Mohammadalinejhad, S., Almasi, H., and Moradi, M., 2020. Immobilization of Echium amoenum anthocyanins into bacterial cellulose film: A novel colorimetric pH indicator for freshness/spoilage monitoring of shrimp. Food Control, 113: 107169, DOI: https://doi.org/10.1016/j.foodcont.2020.107169.
Morsy, M. K., Zór, K., Kostesha, N., Alstrøm, T. S., Heiskanen, A., El-Tanahi, H., et al., 2016. Development and validation of a colorimetric sensor array for fish spoilage monitoring. Food Control, 60: 346–352, DOI: https://doi.org/10.1016/j.foodcont.2015.07.038.
Ohta, N., 1977. Correspondence between CIELAB and CIELUV color differences. Color Research & Application, 2(4): 178–182.
Ojagh, S. M., Rezaei, M., Razavi, S. H., and Hosseini, S. M. H., 2010. Effect of chitosan coatings enriched with cinnamon oil on the quality of refrigerated rainbow trout. Food Chemistry, 120(1): 193–198, DOI: https://doi.org/10.1016/j.foodchem.2009.10.006.
Pacquit, A., Frisby, J., Diamond, D., Lau, K., Farrell, A., Quilty, B., et al., 2007. Development of a smart packaging for the monitoring of fish spoilage. Food Chemistry, 102(2): 466–470, DOI: https://doi.org/10.1016/j.foodchem.2006.05.052.
Prabhakar, P. K., Vatsa, S., Srivastav, P. P., and Pathak, S. S., 2020. A comprehensive review on freshness of fish and assessment: Analytical methods and recent innovations. Food Research International, 133: 109157, DOI: https://doi.org/10.1016/j.foodres.2020.109157.
Prietto, L., Mirapalhete, T. C., Pinto, V. Z., Hoffmann, J. F., Vanier, N. L., Lim, L. T., et al., 2017. pH-sensitive films containing anthocyanins extracted from black bean seed coat and red cabbage. LWT — Food Science and Technology, 80: 492–500, DOI: https://doi.org/10.1016/j.lwt.2017.03.006.
Quan, Z., He, H., Zhou, H., Liang, Y., Wang, L., Tian, S., et al., 2021. Designing an intelligent nanofiber ratiometric fluorescent sensor sensitive to biogenic amines for detecting the freshness of shrimp and pork. Sensors and Actuators B: Chemical, 333: 129535, DOI: https://doi.org/10.1016/j.snb.2021.129535.
Rukchon, C., Nopwinyuwong, A., Trevanich, S., Jinkarn, T., and Suppakul, P., 2014. Development of a food spoilage indicator for monitoring freshness of skinless chicken breast. Talanta, 130: 547–554, DOI: https://doi.org/10.1016/j.talanta.2014.07.048.
Sani, M. A., Tavassoli, M., Hamishehkar, H., and McClements, D. J., 2021. Carbohydrate-based films containing pH-sensitive red barberry anthocyanins: Application as biodegradable smart food packaging materials. Carbohydrate Polymers, 255: 117488, DOI: https://doi.org/10.1016/j.carbpol.2020.117488.
Shi, C., Qian, J., Zhu, W., Liu, H., Han, S., and Yang, X., 2019. Nondestructive determination of freshness indicators for tilapia fillets stored at various temperatures by hyperspectral imaging coupled with RBF neural networks. Food Chemistry, 275: 497–503, DOI: https://doi.org/10.1016/j.foodchem.2018.09.092.
Shi, C., Yang, X., Han, S., Fan, B., Zhao, Z., Wu, X., et al., 2018. Nondestructive prediction of tilapia fillet freshness during storage at different temperatures by integrating an electronic nose and tongue with radial basis function neural networks. Food and Bioprocess Technology, 11(10): 1840–1852, DOI: https://doi.org/10.1007/s11947-018-2148-8.
Siró, I., 2012. Active and intelligent packaging of food. In: Progress in Food Preservation. Bhat, R., et al., eds., John Wiley & Sons Ltd., Chichester, 23–48.
Siu, G. M., and Draper, H., 1978. A survey of the malonaldehyde content of retail meats and fish. Journal of Food Science, 43(4): 1147–1149.
Sun, J., Zhang, R., Zhang, Y., Liang, Q., Zhang, F., Xu, P., et al., 2020. Evaluation of fish freshness using impedance spectroscopy based on the characteristic parameter of orthogonal direction difference. Journal of the Science of Food and Agriculture, 100(11): 4124–4131, DOI: https://doi.org/10.1002/jsfa.10435.
Tassanawatm, S., Phandee, A., Magaraphan, R., Nithitanakul, M., and Manuspiya, H., 2007. pH-sensitive PPClay nanocomposites for beverage smart packaging. Proceedings of the 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems. IEEE NEMS 2007, 478–482.
Vanegas, D. C., Patino, L., Mendez, C., Oliveira, D. A., Torres, A. M., Gomes, C. L., et al., 2018. Laser scribed graphene biosensor for detection of biogenic amines in food samples using locally sourced materials. Biosensors (Basel), 8(2): 42, DOI: https://doi.org/10.3390/bios8020042.
Wells, N., Yusufu, D., and Mills, A., 2019. Colourimetric plastic film indicator for the detection of the volatile basic nitrogen compounds associated with fish spoilage. Talanta, 194: 830–836, DOI: https://doi.org/10.1016/j.talanta.2018.11.020.
Wu, L., Pu, H., and Sun, D. W., 2019. Novel techniques for evaluating freshness quality attributes of fish: A review of recent developments. Trends in Food Science & Technology, 83: 259–273, DOI: https://doi.org/10.1016/j.tifs.2018.12.002.
Yusufu, D., Wang, C., and Mills, A., 2018. Evaluation of an ‘After Opening Freshness (AOF)’ label for packaged ham. Food Packaging and Shelf Life, 17: 107–113, DOI: https://doi.org/10.1016/j.fpsl.2018.06.002.
Zhai, X., Zou, X., Shi, J., Huang, X., Sun, Z., Li, Z., et al., 2020. Amine-responsive bilayer films with improved illumination stability and electrochemical writing property for visual monitoring of meat spoilage. Sensors and Actuators B: Chemical, 302: 127130, DOI: https://doi.org/10.1016/j.snb.2019.127130.
Zhang, J., Huang, X., Shi, J., Liu, L., Zhang, X., Zou, X., et al., 2021. A visual bi-layer indicator based on roselle anthocyanins with high hydrophobic property for monitoring griskin freshness. Food Chemistry, 355: 129573, DOI: https://doi.org/10.1016/j.foodchem.2021.129573.
Zhang, J., Zou, X., Zhai, X., Huang, X., Jiang, C., and Holmes, M., 2019. Preparation of an intelligent pH film based on biodegradable polymers and roselle anthocyanins for monitoring pork freshness. Food Chemistry, 272: 306–312, DOI: https://doi.org/10.1016/j.foodchem.2018.08.041.
Zhong, N., Li, Y. P., Li, X. Z., Guo, C. X., and Wu, T., 2021. Accurate prediction of salmon storage time using improved Raman spectroscopy. Journal of Food Engineering, 293: 110378, DOI: https://doi.org/10.1016/j.jfoodeng.2020.110378.
Zhong, X., Huo, D., Fa, H., Luo, X., Wang, Y., Zhao, Y., et al., 2018. Rapid and ultrasensitive detection of biogenic amines with colorimetric sensor array. Sensors and Actuators B: Chemical, 274: 464–471, DOI: https://doi.org/10.1016/j.snb.2018.07.129.
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This work was supported by the National Key Research and Development Program (No. 2019YFD0901705).
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Wen, H., Ou, C., Tang, H. et al. Development, Characterization and Application of a Three-Layer Intelligent pH-Sensing Indicator Based on Bromocresol Green (BCG) for Monitoring Fish Freshness. J. Ocean Univ. China 22, 565–575 (2023). https://doi.org/10.1007/s11802-023-5326-x
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DOI: https://doi.org/10.1007/s11802-023-5326-x