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Effects of Different Preservation Methods on Physicochemical Property of Marine Pathogen Vibrio anguillarum

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Abstract

Freeze-drying, continuous passage and ultra-low temperature cryopreservation are often used to preserve pathogens. In this study, Vibrio anguillarum was rejuvenated by intramuscular infection as the initial strain. The difference between cells preserved with different preservation methods and their initial strains were compared with physiological and biochemical methods and through antibiotics resistance analysis. The composition of protectants for freeze-drying V. anguillarum was optimized. We found that the optimal composition of protectants was 8% of trehalose, 12% of skim milk, 8.0% of lactose, 2.0% of sodium citrate, 12.0% of serum and 8.0% of mannitol. The indexes of lysine decarboxylase and urease changed after continuous passage. The urease reaction changed after freeze-drying and freeze-thawing, but the reaction can be restored to the initial after freeze-drying. Based on the antibiotics resistance analyses, the sensitivity of V. anguillarum to different drugs including rifampicin, erythrocin, furazolidone, ceftazidime, lomefloxacin, gentamycin, azithromycin, doxycycline, ampicillin, co-trimoxazole and cefoperazone changed after different treatments, and some of these changes can be restored to the original through activation culture. In sum, compared with cryopreservation and continuous passage, the freeze-drying is more sustainable for the long-term preservation of V. anguillarum, which showed a better effect in maintaining the original characteristics of pathogen.

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Acknowledgements

This work was funded by the Key Projects of Science and Technology Innovation of Shandong Province (No. 2018YFJH0703), the Central Public-Interest Scientific Institution Basal Research Fund, Yellow Sea Fisheries Research Institutes, CAFS (No. 20603022017004), the Projects of International Exchange and Cooperation in Agriculture, Ministry of Agriculture and Rural Affairs of China-Science, Technology and Innovation Cooperation in Aquaculture with Tropical Countries.

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Authors and Affiliations

  1. Laboratory for Marine Fisheries Science and Food Production Processes, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071, China

    Yongxiang Yu, Zheng Zhang, Yingeng Wang, Meijie Liao, Xiaojun Rong, Bin Li & Hao Zhang

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  1. Yongxiang Yu
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  2. Zheng Zhang
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  6. Bin Li
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Correspondence to Zheng Zhang or Yingeng Wang.

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Yu, Y., Zhang, Z., Wang, Y. et al. Effects of Different Preservation Methods on Physicochemical Property of Marine Pathogen Vibrio anguillarum. J. Ocean Univ. China 18, 1417–1426 (2019). https://doi.org/10.1007/s11802-019-4050-z

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  • DOI: https://doi.org/10.1007/s11802-019-4050-z

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