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Localization and dynamic expression of a 27.8 kDa receptor protein for lymphocystis disease virus infection in sea bass (Lateolabrax japonicus) tissues

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Abstract

Lymphocystis disease virus (LCDV) infects target cells by attaching to a 27.8 kDa receptor (27.8R) protein in flounder Paralichthys olivaceus, and anti-27.8R monoclonal antibodies (MAbs) have been developed. However, the 27.8R existence in tissues of sea bass (Lateolabrax japonicus) and its role in LCDV infection have remained unclear. In this study, the results of western blotting demonstrated that the same 27.8R was shared by flounder and sea bass. LCDV-free sea bass individuals were intramuscularly injected with LCDV, and viral copies were detected in tissues from 3 h post infection and showed a time-dependent increase during 9 days infection. Distribution and synthesis of 27.8R in sea bass tissues were investigated by using anti-27.8R MAbs as probes. It was found that 27.8R was distributed in all the tested tissues. The levels of 27.8R protein were highest in gill and skin, then a bit lowly in stomach, head kidney and heart, followed by spleen, intestine, blood cells, gonad and liver, and least in kidney and brain in healthy sea bass. Upon LCDV infection, 27.8R synthesis was up-regulated in each tissue, and higher in the tissues with higher LCDV copies. The 27.8R and LCDV were detected in some peripheral blood leukocytes but not in red blood cells. These results suggested that 27.8R was widely distributed in sea bass tissues, and it served as a receptor and correlated with tissue tropism of LCDV infection. Furthermore, leukocytes had the potential of being a LCDV carrier and were responsible for a systemic infection of LCDV in sea bass.

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Acknowledgements

This study is supported by the National Natural Science Foundation of China (Nos. 31472295 and 31672685), Science and Technology Development Project of Shandong Province (No. 2014GNC111015) and Taishan Scholar Program of Shandong Province.

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Authors and Affiliations

  1. Laboratory of Pathology and Immunology of Aquatic Animals, Ocean University of China, Qingdao, 266003, P. R. China

    Ronghua Wu, Xiuzhen Sheng, Xiaoqian Tang, Jing Xing & Wenbin Zhan

  2. Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266071, P. R. China

    Wenbin Zhan

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  1. Ronghua Wu
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  2. Xiuzhen Sheng
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  3. Xiaoqian Tang
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  4. Jing Xing
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  5. Wenbin Zhan
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Correspondence to Wenbin Zhan.

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Wu, R., Sheng, X., Tang, X. et al. Localization and dynamic expression of a 27.8 kDa receptor protein for lymphocystis disease virus infection in sea bass (Lateolabrax japonicus) tissues. J. Ocean Univ. China 16, 889–896 (2017). https://doi.org/10.1007/s11802-017-3280-1

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  • DOI: https://doi.org/10.1007/s11802-017-3280-1

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