Skip to main content
Log in

Dietary protein requirement of juvenile turbot (Scophthalmus maximus Linnaeus)

  • Published:
Journal of Ocean University of China Aims and scope Submit manuscript

Abstract

The dietary protein requirement of juvenile turbot (initial average weight, 38.2 g ± 0.1 g) reared indoor in aerated aquaria was determined in this study. Five energy equal experimental diets were formulated with fish meal as protein source, which contained different concentrations of protein (47.2%, 51.0%, 54.6%, 59.3% and 63.6% of dry diet). Three groups of fish with 18 individuals in each, were cultured in 300-L tanks and fed twice a day for 8 weeks. During culture, temperature was controlled between 15.0 and 18.0°C, salinity was controlled between 28.5 and 32.0, acidity was controlled between pH7.8 and pH8.5, and ammonia nitrogen was maintained below 0.03 mg L−1 and dissolved oxygen was maintained about 7 mg L−1. Results showed that the growth of fish was significantly affected by dietary protein content (P < 0.05). Specific growth rate (SGR) of turbot increased when dietary protein content varied between 47.2% and 51.0% (P < 0.05), and then kept stable when dietary protein content was higher than 51.0%. Fish which were fed the diet containing 63.6% protein showed the highest SGR while those fed the diet containing 59.3% protein showed the highest feed efficiency rate. No significant difference of feed intake and protein efficiency ratio was found among experimental diets (P > 0.05). Broken-line regression analysis of SGR showed that the optimal dietary protein requirement of turbot was about 57.0%.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Adron, J. W., Blair, A., Cowey, C. B., and Shanks, A. M., 1976. Effects of dietary energy level and dietary energy source on growth, feed conversion and body composition of turbot (Scophthalmus maximus L.). Aquaculture, 7: 125–132.

    Article  Google Scholar 

  • Alam, M. S., Teshima, S. I., Koshio, S., Uyan, O., and Ishikawa, M., 2004. Effects of dietary protein and lipid levels on growth and body composition of juvenile Japanese flounder, Paralichthys olivaceus, fed diets containing intact proteins or crystalline amino acids. Journal of Applied Aquaculture, 14: 115–131.

    Article  Google Scholar 

  • Allan, G. L., Johnson, R. J., Booth, M. A., and Stone, D. J., 2001. Estimating digestible protein requirements of silver perch, Bidyanus bidyanus Mitchell. Aquaculture Research, 32: 337–347.

    Article  Google Scholar 

  • Association of Official Analytical Chemists (AOAC), 1990. Official Methods of Analysis. 15th edition. Association of Official Analytical Chemists, Arlington, VA, 1298pp.

    Google Scholar 

  • Caceres-Martinez, C., Cadena-Roa, M., and Metailler, R., 1984. Nutritional requirements of turbot (Scophthalmus maximus): I. A preliminary study of protein and lipid utilization. Journal of the World Marine Society, 15: 191–202.

    Article  Google Scholar 

  • Coutinho, F., Peres, H., Guerreiro, I., Pousao-Ferreira, P., and Oliva-Teles, A., 2012. Dietary protein requirement of sharpsnout sea bream (Diplodus puntazzo, Cetti 1777) juveniles. Aquaculture, 356–357: 391–397.

    Article  Google Scholar 

  • Cowey, C. B., Pope, J. A., Asron, J. W., and Blair, A., 1972. Studies on the nutrition of marine flatfish. The protein requirement of plaice (Pleuronectes platessa). The British Journal of Nutrition, 28: 447–456.

    Article  Google Scholar 

  • Craig, S. R., Schwarz, M. H., and McLean, E., 2006. Juvenile cobia (Rachycentron canadum) can utilize a wide range of protein and lipid levels without impacts on production characteristics. Aquaculture, 261: 384–391.

    Article  Google Scholar 

  • Deng, D., Ju, Z. Y., Dominy, W., Murashige, R., and Wilson, R. P., 2011. Optimal dietary protein levels for juvenile Pacific threadfin (Polydactylus sexfilis) fed diets with two levels of lipid. Aquaculture, 316: 25–30.

    Article  Google Scholar 

  • Gonzaleza, S. R., Craig, E., McLean, M. H., Schwarz, M. H., and Flick, G. J., 2005. Dietary protein requirement of southern flounder, Paralichthys lethostigma. Journal of Applied Aquaculture, 17: 37–50.

    Article  Google Scholar 

  • Guo, Z. Q., Zhu, X. M., Liu, J. S., Han, D., Yang, Y. X., Lan, Z. Q., and Xie, S. Q., 2012. Effects of dietary protein level on growth performance, nitrogen and energy budget of juvenile hybrid sturgeon, Acipenser baerii ♀×A. gueldenstaedtii ♂. Aquaculture, 338–341: 89–95

    Article  Google Scholar 

  • Hebb, C. D., Castell, J. D., Anderson, D. M., and Batt, J., 2003. Growth and feed conversion of juvenile winter flounder (Pleuronectes americanus) in relation to different protein-to-lipid levels in isocaloric diets. Aquaculture, 221: 439–449.

    Article  Google Scholar 

  • Kim, S. S., and Lee, K. J., 2009. Dietary protein requirement of juvenile tiger puffer (Takifugu rubripes). Aquaculture, 287: 219–222.

    Article  Google Scholar 

  • Lee, J. K., Cho, S. H., Park, S. U., Kim, K. D., and Lee, S. M., 2003. Dietary protein requirement for young turbot (Scophthalmus maximus L.). Aquaculture Nutrition, 9: 283–286.

    Article  Google Scholar 

  • Lee, S. M., Cho, S. H., and Kim, K. D., 2000. Effects of dietary protein and energy levels on growth and body composition of juvenile flounder (Paralichthys olivaceus). Journal of World Aquaculture Society, 31: 306–315.

    Article  Google Scholar 

  • Lee, S. M., Lee, J. H., Kim, K. D., Kim, K. D., and Lee, S. M., 2006. Optimum dietary protein for growth of juvenile starry flounder, Platichthys stellatus. Journal of the World Aquaculture Society, 37: 200–203.

    Article  Google Scholar 

  • Luo, Z., Liu, Y., Mai, K., Tian, L., Liu, D., and Tan, X., 2004. Optimal dietary protein requirement of grouper Epinephelus coioides juveniles fed isoenergetic diets in floating net cages. Aquaculture Nutrition, 10: 247–252.

    Article  Google Scholar 

  • Martinez-Palacios, C. A., Harfush-Melendez, M., Chavez-Sanchez, C., and Ross, L. G., 1996. The optimum dietary protein level for the Mexican cichlid Cichlasoma urophthalmus (Gunther): A comparison of estimates derived from experiments using fixed-rate feeding and satiation feeding. Aquaculture Nutrition, 2: 11–20.

    Article  Google Scholar 

  • Moore, B. J., Hung, S. S. O., and Medrano, J. F., 1988. Protein requirement of hatchery-produced juvenile white sturgeon (Acipenser transmontanus). Aquaculture, 71: 235–245.

    Article  Google Scholar 

  • Ng, W. K., Abdullah, N., and De Silva, S. S., 2008. The dietary protein requirement of the Malaysian mahseer, Tor tambroides (Bleeker), and the lack of protein-sparing action by dietary lipid. Aquaculture, 284: 201–206.

    Article  Google Scholar 

  • Ng, W. K., Soon, S. C., and Hashim, R., 2001. The dietary protein requirement of a bagrid catfish, Mystus nemurus (Cuvier & Valenciennes), determined using semipurified diets of varying protein level. Aquaculture Nutrition, 7: 45–51.

    Article  Google Scholar 

  • Shearer, K. D., 1994. Factors affecting the proximate composition of cultured fishes with emphasis on salmonids. Aquaculture, 119: 63–88.

    Article  Google Scholar 

  • Wilson, R. P., 2002. Amino acids and proteins. In: Fish Nutrition. 3rd edition. Halver, J. E., and Hardy, R. W., eds., Academic Press, San Diego, CA, 144–175.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Qinghui Ai.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Liu, X., Mai, K., Liufu, Z. et al. Dietary protein requirement of juvenile turbot (Scophthalmus maximus Linnaeus). J. Ocean Univ. China 14, 325–328 (2015). https://doi.org/10.1007/s11802-015-2360-3

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11802-015-2360-3

Key words

Navigation