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Analysis of genetic diversity and differentiation of seven stocks of Litopenaeus vannamei using microsatellite markers

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Abstract

Seven microsatellite markers were used to evaluate the genetic diversity and differentiation of seven stocks of Litopenaeus vannamei, which were introduced from Central and South America to China. All seven microsatellite loci were polymorphic, with polymorphism information content (PIC) values ranging from 0.593 to 0.952. Totally 92 alleles were identified, and the number of alleles (Na) and effective alleles (Ne) varied between 4 and 21 and 2.7 and 14.6, respectively. Observed heterozygosity (H o) values were lower than the expected heterozygosity (H e) values (0.526–0.754), which indicated that the seven stocks possessed a rich genetic diversity. Thirty-seven tests were detected for reasonable significant deviation from Hardy-Weinberg equilibrium. F is values were positive at five loci, suggesting that there was a relatively high degree of inbreeding within stocks. Pairwise F st values ranged from 0.0225 to 0.151, and most of the stock pairs were moderately differentiated. Genetic distance and cluster analysis using UPGMA revealed a close genetic relationship of L. vannamei between Pop2 and Pop3. AMOVA indicated that the genetic variation among stocks (11.3%) was much lower than that within stocks (88.7%). Although the seven stocks had a certain degree of genetic differentiation and a rich genetic diversity, there is an increasing risk of decreased performance due to inbreeding in subsequent generations.

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Authors and Affiliations

  1. College of Marine Life Sciences, Key Laboratory of Marine Genetics and Breeding (Ministry of Education), Ocean University of China, Qingdao, 266003, P. R. China

    Kai Zhang & Quanqi Zhang

  2. Key Laboratory for Sustainable Utilization of Marine Fisheries Resources (Ministry of Agriculture), Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071, P. R. China

    Weiji Wang, Weiya Li & Jie Kong

  3. Shandong Marine Fishing and Production Management Station, Yantai, 264000, P. R. China

    Weiya Li

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  1. Kai Zhang
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  2. Weiji Wang
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  3. Weiya Li
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  4. Quanqi Zhang
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  5. Jie Kong
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Correspondence to Jie Kong.

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Zhang, K., Wang, W., Li, W. et al. Analysis of genetic diversity and differentiation of seven stocks of Litopenaeus vannamei using microsatellite markers. J. Ocean Univ. China 13, 647–656 (2014). https://doi.org/10.1007/s11802-014-2208-2

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  • DOI: https://doi.org/10.1007/s11802-014-2208-2

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