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Molecularly imprinted polymer based on upconversion nanoparticles for highly selective and sensitive determination of Ochratoxin A

上转换荧光传感检测赭曲霉毒素A的研究

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Abstract

A novel molecularly imprinted polymer (MIP) based on upconversion nanoparticles (UCNPs) was successfully synthesized for determination of Ochratoxin A (OTA). The MIP was developed on the silica-coated UCNPs using N-(1-hydroxy-2-naphthoyl amido)-(L)-phenylalanine (HNA-Phe) as the alternative template. The final composite combined the advantages of the high selectivity of MIP with the high fluorescence intensity of UCNPs which was selective and sensitive to OTA. Under the optimal condition, the fluorescence intensity of UCNPs@SiO2@MIP decreases linearly when the concentration of OTA increases from 0.05 to 1.0 mg/L. The detection limit of OTA with the method was 0.031 mg/L. At three spiked concentration levels (50, 100 and 200 µg/kg), the recovery ranges of OTA in corn, rice and feed are 88.0%–91.6%, 80.2%–91.6% and 89.2%–90.4%, respectively.

摘要

本文基于分子印迹技术的高选择性和上转换纳米材料的荧光特性, 以赭曲霉毒素A(OTA)的结构类似物HNA-Phe为模板, 开发了一种能高选择、 高灵敏地识别痕量OTA的分子印迹聚合物 (UCNPs@SiO2@MIP)。 在最优条件下, 当OTA浓度为0.05~1.0mg/L时, 荧光印迹聚合物的荧光猝灭程度与OTA的浓度呈现良好的线性关系。 建立了 OTA 的荧光传感检测方法, 该方法的最低检出限为 0.031 mg/L。 当OTA的加标浓度为50、 100和200昭 μg/kg时, 大米、 玉米和饲料中OTA的回收范围分别为 80.2%~91.6%(RSD<4.6%)、 89.2%~90.4% (RSD<5.5%) 和 88.0%~91.6% (RSD<5.4%)。

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Authors and Affiliations

  1. State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin, 300457, China

    Zhen Yan  (闫祯) & Guo-zhen Fang  (方国臻)

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  1. Zhen Yan  (闫祯)
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  2. Guo-zhen Fang  (方国臻)
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Correspondence to Guo-zhen Fang  (方国臻).

Additional information

Foundation item: Project(17ZYPTJC00050) supported by Science and Technology Committee of Tianjin, China; Project(2017YFC1600803) supported by the Ministry of Science and Technology of China

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Yan, Z., Fang, Gz. Molecularly imprinted polymer based on upconversion nanoparticles for highly selective and sensitive determination of Ochratoxin A. J. Cent. South Univ. 26, 515–523 (2019). https://doi.org/10.1007/s11771-019-4023-9

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  • DOI: https://doi.org/10.1007/s11771-019-4023-9

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