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Characterization of Microcystis Aeruginosa immobilized in complex of PVA and sodium alginate and its application on phosphorous removal in wastewater

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Abstract

Based on ecological niche theory, Microcystis Aeruginosa (MA) immobilized in the complex of polyvinyl alcohol (PVA) and sodium alginate (SA) crosslinked by CaCl2, was treated as a new kind of special species, and its properties were investigated. Chlorophyll a was used to characterize the bioactivity of the immobilized MA. Results reveal that the gel beads have mechanical strength and chemical stability even under non-sterile harsh conditions, which may be attributed to the rarely seen structure (including three different layers: dense surface, tubular-shaped divergent structure and honeycomb crystal lattice layer) of the immobilized MA determined by scanning electron microscope (SEM). SEM also displays that more quantity of MA is attached to the inwall after cultivation, which demonstrates that the MA within beads maintains high bioactivity. Removal capacities on phosphorous (P) removal in wastewater in the presence and absence of the BG-11 medium were examined, and the removal ratios are 80.3% and 76.7%, respectively, which indicates that the beads without providing ample nutrients still have high capacity of P removal. In addition, control experiment, utilizing polyvinyl alcohol and sodium alginate (PVA-SA) beads without immobilized MA, demonstrates that MA within beads plays the key role in absorbing P.

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Authors and Affiliations

  1. College of Environmental Science and Engineering, Hunan University, Changsha, 410082, China

    Fei Li  (李飞), Wen-juan Mao  (毛文娟), Xiao-yu Wang  (王晓钰), Zhi-hua Xiao  (肖智华), Yao-yu Zhou  (周耀渝) & Guang-ming Zeng  (曾光明)

  2. Key Laboratory of Environmental Biology and Pollution Control of Ministry of Education, Hunan University, Changsha, 410082, China

    Fei Li  (李飞), Wen-juan Mao  (毛文娟), Xiao-yu Wang  (王晓钰), Zhi-hua Xiao  (肖智华), Yao-yu Zhou  (周耀渝) & Guang-ming Zeng  (曾光明)

  3. Department of Bioengineering and Environmental Science, Changsha University, Changsha, 410003, China

    Xue Li  (李雪)

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  1. Fei Li  (李飞)
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Correspondence to Xue Li  (李雪).

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Foundation item: Projects(51178172, 51308076) supported by the National Natural Science Foundation of China; Project(13JJ4107) supported by Hunan Provincial Natural Science Foundation, China; Project(K1207026-31) supported by Changsha Planning Project of Science and Technology, China

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Li, F., Mao, Wj., Li, X. et al. Characterization of Microcystis Aeruginosa immobilized in complex of PVA and sodium alginate and its application on phosphorous removal in wastewater. J. Cent. South Univ. 22, 95–102 (2015). https://doi.org/10.1007/s11771-015-2499-5

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  • DOI: https://doi.org/10.1007/s11771-015-2499-5

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