Abstract
Ulcerative colitis (UC) is an idiopathic chronic inflammatory bowel disease. The effect of current therapy is far from satisfactory; therefore, it is necessary to develop new UC therapeutic strategies. Lactiplantibacillus plantarum 21 (LAB 21) has a protective effect in UC. We investigated the underlying mechanisms of action of LAB 21 using UC rat models established by 2,4,6-trinitrobenzenesulfonic acid. The results showed that LAB 21 administration reduced the disease activity index, macroscopic scoring, and histopathological damage scores in UC rats. UC rats had up-regulated levels of tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-6, and IL-17A, and an increased percentage of T helper 17 (Th17) cells, while LAB 21 treatment inhibited the up-regulation of inflammatory cytokines level and Th17 cell proportion. Moreover, LAB 21 administration attenuated the decrease of E-cadherin, ZO-1, and Occludin proteins in UC rats, and inhibited toll-like receptor 4 (TLR4) expression and nuclear factor (NF)-κB nuclear translocation. In conclusion, this study demonstrates that LAB 21 exerts a protective effect in UC rats by inhibiting the TLR4/NF-κB pathway and inflammatory response, down-regulating the proportion of Th17 cells, and reducing intestinal permeability.
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Abbreviations
- UC:
-
Ulcerative colitis
- IBD:
-
Idiopathic chronic inflammatory bowel disease
- LAB 21:
-
Lactiplantibacillus plantarum 21
- TNF-α:
-
Tumor necrosis factor-α
- IL:
-
Interleukin
- Th17:
-
T helper 17
- NF:
-
Nuclear factor
- TLR4:
-
Toll-like receptor 4
- TNBS:
-
2,4,6-Trinitrobenzenesulfonic acid
- ELISA:
-
Enzyme-linked immunosorbent assay
- FITC:
-
Fluorescein isothiocyanate
- HRP:
-
Horseradish peroxidase
- ECL:
-
Electrochemiluminescence
- DAPI:
-
4′,6-Diamidino-2-phenylindole
- ANOVA:
-
Analysis of variance (ANOVA)
- SD:
-
Standard deviation
- DAI:
-
Disease activity index
- MS:
-
Macroscopic scoring
- HDS:
-
Histopathological damage scores
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Funding
This study was supported by Natural Science Foundation of Zhejiang Province (LY17H030008), Medical and health science and technology plan of Zhejiang Province (2017KY190), and Medical and health science and technology plan of Zhejiang Province (2019KY253).
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All animal experimental procedures were approved by by the Institutional Animal Ethics Committee of Zhejiang Hospital (protocol number: 2017–033).
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Wang, WF., Zhou, F., Xu, S. et al. Lactiplantibacillus plantarum attenuates 2,4,6-trinitrobenzenesulfonic acid-induced ulcerative colitis in rats by regulating the inflammatory response, T helper 17 immune response, and intestinal permeability. Biologia 77, 2667–2676 (2022). https://doi.org/10.1007/s11756-022-01111-w
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DOI: https://doi.org/10.1007/s11756-022-01111-w