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Circ_0024108 promotes the progression of esophageal cancer cells

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Abstract

Background

Esophageal squamous cell carcinoma (ESCC) is a serious malignant cancer. The treatment effect of ESCC is relatively poor and needs further improvement. According to reports, circular RNAs (circRNAs) actively participate in human carcinogenesis. More explorations are needed about the action of circRNAs in ESCC.

Methods

Circ_0024108, miR-488-3p, and USP14 was quantified by a qRT-PCR or immunoblotting method. Cell proliferation evaluation was performed by MTT, EdU, and colony formation assays. Evaluation of cell motility and invasiveness was conducted using wound healing assay and transwell assay. The regulatory mechanism of circ_0024108, miR-488-3p, and USP14 was detected by RNA pull-down assay and dual-luciferase reporter assay.

Results

Circ_0024108 and USP14 were significantly overexpressed in ESCC, while miR-488-3p was underexpressed. Deficiency of circ_0024108 impeded cell growth, motility, and invasiveness. Circ_0024108 regulated the expression of USP14 in ESCC cells via miR-488-3p. Also, circ_0024108 was present at high levels in serum exosomes from ESCC patients with high specificity and sensitivity.

Conclusions

Taken together, circ_0024108 participated in the progress of ESCC through the miR-488-3p/USP14 axis. Circ_0024108 was differentially expressed in serum exosomes. Circ_0024108 might be a potential biomarker for the diagnosis of ESCC.

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Data availability

All data generated or analysed during this study are included in this published article (and its supplementary information files).

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Acknowledgements

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Author notes

  1. Tongchen Hu and Huali Peng contributed equally to the current work.

Authors and Affiliations

  1. Department of Thoracic Surgery, The Affiliated Hospital of Southwest Medical University, No. 25 TaiPing St, Jiangyang District, Luzhou, 646000, Sichuan, People’s Republic of China

    Tongchen Hu & Jintao He

  2. Department of Thoracic Surgery, The People’s Hospital of Leshan, Leshan, 614000, Sichuan, China

    Huali Peng, Fan Yang & Fan Zhang

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  1. Tongchen Hu
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Correspondence to Jintao He.

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Supplementary Information

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11748_2023_1909_MOESM1_ESM.tif

Supplementary file1 (TIF 688 KB) Figure S1 Effect of circ_0024108 depletion on PCNA expression in ESCC cells. The protein expression of PCNA was measured by immunoblotting in ECA109 and KYSE410 cells transfected as indicated. The data are presented as the mean ± SD; *P < 0.05.

11748_2023_1909_MOESM2_ESM.tif

Supplementary file2 (TIF 688 KB) Figure S2. Identification and expression detection after exosomes treatment. (A) circ_0024108 expression in si-circ_0024108 and oe-circ_0024108 exosomes of ECA109 cells. (B) miR-488-3p expression in KYSE410 cells after co-culture with si-circ_0024108 and oe-circ_0024108 exosomes detected by qRT-PCR. (C and D) USP14 expression in KYSE410 cells after co-culture with si-circ_0024108 and oe-circ_0024108 exosomes detected by qRT-PCR and immunoblotting. The data are presented as the mean ± SD; qRT-PCR, quantitative real-time polymerase chain reaction; *P < 0.05.

11748_2023_1909_MOESM3_ESM.tif

Supplementary file3 (TIF 371 KB) Figure S3. The regulation of sh-circ_0024108 in the expression of miR-488-3p and USP14 in the xenograft tumors. (A) miR-488-3p expression by qRT-PCR in the xenograft tumors transduced with sh-NC or sh-circ_0024108. (B) USP14 protein level by immunoblotting in the xenograft tumors. The data are presented as the mean ± SD; qRT-PCR, quantitative real-time polymerase chain reaction; *P < 0.05.

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Hu, T., Peng, H., Yang, F. et al. Circ_0024108 promotes the progression of esophageal cancer cells. Gen Thorac Cardiovasc Surg 71, 418–431 (2023). https://doi.org/10.1007/s11748-023-01909-8

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