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Effects of Variety, Maturation and Growing Region on Chemical Properties, Fatty Acid and Sterol Compositions of Virgin Olive Oils

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Journal of the American Oil Chemists' Society

Abstract

Chemical properties, fatty acid and sterol compositions of olive oils extracted from Gemlik and Halhalı varieties grown in Hatay and Mardin provinces in Turkey were investigated during four maturation stages. The olive oil samples were analyzed for their chemical properties such as free acidity, peroxide value, total carotenoid, total chlorophyll, total phenolic contents, antioxidant activity, fatty acid and sterol compositions. Chemical properties, fatty acids and sterol profiles of olive oil samples generally showed statistically significant differences depending on the varieties, maturation and growing areas (p < 0.05). As free fatty acid contents and total phenolic contents increased, total carotenoid and chlorophyll contents decreased throughout the maturity stages. Total carotenoid and chlorophyll contents of oil samples from Mardin were higher than those of Hatay. The total phenolic compounds of olive oil samples ranged from 20.62 in Gemlik to 525.22 mg GAE/kg oil in Halhalı from Hatay. In general, the phenolic contents and antioxidant activities of olive oil samples were positively associated. Oleic acid content was the highest 71.53 % in H1 samples in Hatay. Total sterol contents were 1194.33 mg/kg in Halhalı and 2008.66 mg/kg in Gemlik from Hatay. Stigmasterol contents of oils obtained from Hatay were lower than those of Mardin. Oleic acid, palmitic acid, β-sitosterol, ∆-5-avenasterol and campesterol contents fluctuated with maturation for each of variety from both growing regions. These results showed that the variety, growing area and maturation influence the chemical properties, fatty acid and sterol compositions.

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References

  1. Temime SB, Manai H, Methenni K, Baccouri B, Abaza L, Daoud D, Sanchez Casas J, Bueno EO, Zarrouk M (2008) Sterolic composition of Chetoui virgin olive oil: influence of geographical origin. Food Chem 110:368–374

    Article  Google Scholar 

  2. Baiano A, Terracone C, Viggiani I, Del Nobile MA (2013) Effects of cultivars and location on quality, phenolic content and antioxidant activity of extra-virgin olive oils. J Am Oil Chem Soc 90:103–111

    Article  CAS  Google Scholar 

  3. Fernandez MDL, Vargas VCS, Silva MF (2014) Phenolic compounds and antioxidant capacity of monovarietal olive oils produced in Argentina. J Am Oil Chem Soc 91:2021–2033

    Article  Google Scholar 

  4. Visioli F, Poli A, Galli C (2002) Antioxidant and other biological activities of phenols from olives and olive oil. Med Res Rev 22(1):65–75

    Article  CAS  Google Scholar 

  5. Ranalli A, Lucera L, Contento S, Simone N, Del Re P (2004) Bioactive constituents, flavors and aromas of virgin oils obtained by processing olives with a naturel enzyme extract. Eur J Lipid Sci Technol 106:187–197

    Article  CAS  Google Scholar 

  6. Pelle DF, Vilela D, Gonzalez MC, Sterzo CL, Compagnone D, Carlo MD, Escarpa A (2015) Antioxidant capacity index based on gold nanoparticles formation. Application to extra virgin olive oil samples. Food Chem 178:70–75

    Article  Google Scholar 

  7. Del Monaco G, Officioso A, D’Angelo S, La Cara F, Ionata E, Marcolongo L, Squillaci G, Maurelli L, Morana A (2015) Characterization of extra virgin olive oils produced with typical Italian varieties by their phenolic profile. Food Chem 184:220–228

    Article  Google Scholar 

  8. Rotondi A, Magli M (2004) Ripening of olives var. correggiolo: modification of oxidative stability of oils during fruit ripening and oil storage. J of Food Agric Envir 2(3 and 4):193–199

    CAS  Google Scholar 

  9. BOSKOU D (1996) Olive oil chemistry and technology. AOCS press, Champaign, pp 12–52, 161 s

  10. Lerma-Garcia MJ, Lantano C, Chiavaro E, Cerretani L, Herrero-Martinez JM, Simo-Alfonso EF (2009) Classification of extra virgin olive oils according to their geographical origin using phenolic compound profiles obtained by capillary electrochromatography. Food Res Int 42:1446–1452

    Article  CAS  Google Scholar 

  11. Lukic M, Lukic I, Krapac M, Sladonja B, Pilizota V (2013) Sterols and triterpene diols in olive oil as indicators of variety and degree of ripening. Food Chem 136:251–258

    Article  CAS  Google Scholar 

  12. Pardo JE, Sena E, Cuesta MA, Granell JD, Valiente J, Alvarez-Ort M (2013) Evaluation of potential and real quality of virgin olive oil from "Campos de Hellin" (Albacete, Spain). J Am Oil Chem Soc 90:851–862

    Article  CAS  Google Scholar 

  13. Cunha SS, Fernandes JO, Beatriz M, Oliveira PP (2006) Quantification of free and esterified sterols in Portuguese olive oils by solid-phase extraction and gas chromatography–mass spectrometry. J Chromatogr A 1128:220–227

    Article  CAS  Google Scholar 

  14. Hatzakis E, Dagounakis G, Agiomyrgianaki A, Dais P (2010) A facile NMR method for the quantification of total, free and esterified sterols in virgin olive oil. Food Chem 122:346–352

    Article  CAS  Google Scholar 

  15. Manai-Djebali H, Krichene D, Ouni Y, Gallardo L, Sanchez J, Osorio E, Daoud D, Guido F, Zarrouk M (2012) Chemical profiles of five minor olive oil varieties grown in central Tunisia. J Food Comp Anal 27:109–119

    Article  CAS  Google Scholar 

  16. Fernandez-Cuesta A, Leon L, Velasco L, De La Rosa R (2013) Chenges in squalene and sterols associated with olive maturation. Food Res Int 54:1885–1889

    Article  CAS  Google Scholar 

  17. Yorulmaz A, Yavuz H, Tekin A (2014) Characterization of Turkish olive oils by triacylglycerol structures and sterol profiles. J Am Oil Chem Soc 91:2077–2090

    Article  CAS  Google Scholar 

  18. Alkan D, Tokatli F, Ozen B (2012) Phenolic characterization and geographical classification of commercial extra virgin olive oils produced in Turkey. J Am Oil Chem Soc 89:261–268

    Article  CAS  Google Scholar 

  19. Kesen S, Kelebek H, Selli S (2014) LC-EIS-MS characterization of phenolic profiles Turkish olive oils as influenced by geographic origin and harvest year. J Am Oil Chem Soc 91:385–394

    Article  CAS  Google Scholar 

  20. Ballus CA, Meinhart AD, De Souza Campos FA Jr, Godoy HT (2015) Total phenolics of virgin olive oils highly correlate with the hydrogen atom transfer mechanism of antioxidant capacity. J Am Oil Chem Soc 92:843–851

    Article  CAS  Google Scholar 

  21. Sousa C, Gouvinhas I, Barreira D, Carvalho MT, Vilela A, Lopes J, Martins-Lopes P, Barros A (2014) ‘Cobrancosa’ olive oil and drupe: chemical composition at two ripening stages. J Am Oil Chem Soc 91:599–611

    Article  CAS  Google Scholar 

  22. Franco MN, Galeano-Díaz T, López O, Fernández-Bolaños JG, Sánchez J, De Miguel C, Gil MV, Martín-Vertedor D (2014) Phenolic compounds and antioxidant capacity of virgin olive oil. Food Chem 163:289–298

    Article  CAS  Google Scholar 

  23. Beltran G, Aguilera MP, Del Rio C, Sanchez S, Martinez L (2005) Influence of fruit ripening process on the naturel antioxidant content of hojiblanca virgin olive oils. Food Chem 89:207–215

    Article  CAS  Google Scholar 

  24. Criado MN, Romero MP, Casanovas M, Motilva MJ (2008) Pigment profile and colour of monovarietal virgin olive oils from Arbequina cultivar obtained during two consecutive crop seasons. Food Chem 110:873–880

    Article  CAS  Google Scholar 

  25. Psomiadou E, Tsimidou M (2002) Stability of virgin olive oil. 1. Autoxidation studies. J Agric Food Chem 50:716–721

    Article  CAS  Google Scholar 

  26. Velasco J, Dobarganes C (2002) Oxidative stability of virgin olive oil. Eur J Lipid Sci Technol 104:661–676

    Article  CAS  Google Scholar 

  27. Samaniego-Sanchez C, Quesada-Granados JJ, Lopez-Garcia del sSerrana H, Lopez-Martinez MC (2010) β- Carotene, squalene and waxes determined by chromatographic method in picual extra virgin olive oil obtained by a new cold extraction system. J Food Comp Anal 23:671–676

    Article  CAS  Google Scholar 

  28. Abenoza M, Benito M, Oria R, Sanchez-Gimeno AC (2014) Quality characterization of the olive oil from Var. Tosca 07 grown in a commercial high density orchard. J Am Oil Chem Soc 91:613–622

    Article  CAS  Google Scholar 

  29. Aguilera MP, Beltran G, Ortega D, Fernandez A, Jimenez A, Uceda M (2005) Characterisation of virgin olive oil of Italian olive cultivars: “Frantoio” and “Leccino”, grown in Andalusia. Food Chem 89:387–391

    Article  CAS  Google Scholar 

  30. Bozdoğan Konuşkan D, Canbas A (2014) The effects of variety and extraction methods on phenolic compounds and chemical composition of olive oils. Revista de Chim 65(7):788–791

    Google Scholar 

  31. American Oil Chemists’ Society (1998) Official Methods and Recommended Practices of the American Oil Chemists’ Society, 5th edn. AOCS Press, Champaign

    Google Scholar 

  32. Minguez-Mosquera MI, Rejano-Navarro L, Gandul-Rojas B, Sanchez-Gomez AH, Garrido-Fernandez J (1991) J Am Oil Chem Soc 68:332–336

    Article  CAS  Google Scholar 

  33. Pirisi FM, Cabras P, Cao CF, Migliorini M, Magelli M (2000) Phenolic compounds in virgin olive oil. 2. reappraisal of the extraction, HPLC separation, and quantification procedures. J Agric Food Chem 48:1191–1196

    Article  CAS  Google Scholar 

  34. Montedoro G, Servili M, Baldioli M, Miniati E (1992) Simple and hydrolyzable phenolic compounds in virgin olive oil. 1. Their extraction separation and quantitative and semiquantitative evaluation by HPLC. J Agric Food Chem 40:1571–1576

    Article  CAS  Google Scholar 

  35. Brand-Williams W, Culivier ME, Berset C (1995) Use of a free radical method to evaluate antioxidant activity. LWT –. Food Sci Technol 28:25–30

    CAS  Google Scholar 

  36. International Olive Oil Council (2004) Preparation of the fatty acid methyl esters from olive oil and olive pomace oil. COI/T.20/Doc.No.24/Rev.1, 2001, http://www.internationaloliveoil.org. Accessed 7 July 2016

  37. International Olive Oil Council (2004) Determination of the composition and content of sterols by capillary-column gas chromatography. COI/T.20/Doc.No.10/Rev.1,2001, http://www.internationaloliveoil.org. Accessed 7 July 2016

  38. Ozdamar K (1999) Paket Programlar ile İstatistiksel Veri Analizi. Kaan Kitabevi, Eskişehir, p 535s

    Google Scholar 

  39. Condelli N, Caruso MC, Galgano F, Russo D, Milella L (2015) Prediction of the antioxidant activity of extra virgin olive oils produced in the Mediterranean area. Food Chem 177:233–239

    Article  CAS  Google Scholar 

  40. Yorulmaz A, Erinc H, Tekin A (2013) Changes in olive and olive oil characteristics during maturation. J Am Oil Chem Soc 90:647–658

    Article  CAS  Google Scholar 

  41. Arafat SM, Basuniy AMM, Elsayed ME, Soliman HM (2016) Effect of pedological, cultivar and climatic condition on sterols and quality indices of olive oil. Scientia Agric 13(1):23–29

    Google Scholar 

  42. Piravi-Vanak Z, Ghasemi Jahan B, Ghavami M, Ezzatpanah H, Zolfonoun E (2012) The influence of growing region on fatty acids and sterol composition of Iranian olive oils by unsupervised clustering methods. J Am Oil Chem Soc 89:371–378

    Article  CAS  Google Scholar 

  43. Roberedo-Rodriguez P, Gonzalez-Barreiro C, Cancho- Grande B, Simal-Gandara J (2014) Quality of extra virgin olive oils produced in an emerging olive growing area in North-western Spain. Food Chem 164:418–426

    Article  Google Scholar 

  44. Matos LC, Cunha SC, Amaral JS, Pereira JA, Andrade PB, Seabra RM, OLiveira BPP (2007) Chemometric characterization of three varietal olive oils (Cvs. Cobrançosa, Madural and Verdeal Transmontana) extracted from olives with different maturation indices. Food Chem 102:406–414

    Article  CAS  Google Scholar 

  45. Guillaume C, Ravetti L, Lala D, Joshua Johnson R (2012) Technological factors affecting sterols in Australian olive oils. J Am Oil Chem Soc 89:29–39

    Article  CAS  Google Scholar 

  46. Gutierrez F, Jimenez B, Ruiz A, Albi MA (1999) Effects of olive ripeness on the oxidative stability of virgin olive oil extracted from the varieties picual and hojiblanca and on the different components involved. J Agric Food Chem 47:121–127

    Article  CAS  Google Scholar 

  47. Noorali M, Barzegar M, Sahari MA (2014) Sterol and fatty acid compositions of olive oil as an indicator of cultivar and growing area. J Am Oil Chem Soc 91:1571–1581

    Article  CAS  Google Scholar 

  48. Mailer RJ, Ayton J, Graham K (2010) The influence of Growing region, cultivar and harvesting timing on the diversity of Australian olive oil. J Am Oil Chem Soc 87:877–884

    Article  CAS  Google Scholar 

  49. Bajoub A, Hurtado-Fernández E, Ajal EA, Fernández-Gutiérrez A, Carrasco-Pancorbo A, Ouazzani N (2015) Quality and chemical profiles of monovarietal north Moroccan olive oils from ‘‘Picholine Marocaine’’ cultivar: registration database development and geographical discrimination. Food Chem 179:127–136

    Article  CAS  Google Scholar 

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Acknowledgments

The authors give thanks to the Mustafa Kemal University Scientific Investigation Project Office for the financial support of this study, which is part of research project:13520. We are particularly grateful to Dr. Zeki Aydın for antioxidant activity analysis of the olive oil samples.

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Correspondence to Dilsat Bozdogan Konuskan.

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Bozdogan Konuskan, D., Mungan, B. Effects of Variety, Maturation and Growing Region on Chemical Properties, Fatty Acid and Sterol Compositions of Virgin Olive Oils. J Am Oil Chem Soc 93, 1499–1508 (2016). https://doi.org/10.1007/s11746-016-2904-8

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  • DOI: https://doi.org/10.1007/s11746-016-2904-8

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