Abstract
Organic amendments have been utilised as a source of nutrients to improve the growth and yield of many crops, including sweetpotato. However, none of the studies examined the relationship between organic amendments and the initiation of sweetpotato storage roots (SRs). A study was conducted in sweetpotato Orleans cultivar to investigate anatomical changes to roots during storage root formation aiming to elicit if organic amendment affects SR formation by changing soil available nitrogen (N) concentration. Two locally available organic amendments including poultry manure (PM) and sugarcane trash (SCT), were used in this experiment at different rates. Six treatments were included in the study: unamended soil (control), unamended soil with chemical fertiliser, PM 22 Mg/ha, PM 66 Mg/ha, SCT 10 Mg/ha and SCT 30 Mg/ha. Compared with unamended control, SCT application at both rates, 10 or 30 Mg ha−1, significantly promoted the formation of SRs and reduced root lignification, while PM amendment from 22 to 66 Mg ha−1 significantly inhibited SR initiation and enhanced the formation of lignified roots, which appear to be associated to high soil available N caused by PM addition. During the SR initiation, all amended treatments increased N accumulation in plants compared to the control. These results indicated that excessive soil available N introduced by PM amendment could inhibit the formation of SR of sweetpotato. Thus, when applying a lower rate of PM to sweetpotato, and planting should be delayed from 2 to 3 weeks after amending to avoid the detrimental effect on SR initiation. Excessively high rates of PM soil amendment (66 Mg ha−1) should be avoided. A suitable rate of SCT may be applied (~ 10 Mg ha−1 in this study) to promote SR formation of sweetpotato in soil with adequate available N level, but high rates could lead to immobilisation of N and suppress crop growth.
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Introduction
Organic soil amendments are commonly used in agriculture to improve soil organic matter content and other soil properties, which result in a better environment for root growth (Davis and Wilson 2000). In general, organic amendments have been proved to enhance root growth of crops (Donn et al. 2014; Xiao et al. 2016) and result in increased nutrient uptake and yield (Backer et al. 2017; Mehdizadeh et al. 2013; Xiao et al. 2016). The application of soil organic amendments has been demonstrated to improve the yield of many crops such as vegetative, grain, root and tuber crops (Agyarko et al. 2013; Lin et al. 2016; Mehdizadeh et al. 2013; Xiao et al. 2016). The effect of organic amendments on root and tuber crops has received less research attention. Some studies examined the influence of these products on such crops. The application of poultry manure increased cassava yield by around 30% (Ojeniyi et al. 2012). The growth and yield of radish (Raphanus sativus L.) increased significantly when organic soil amendments was in use (Adekiya et al. 2019; Sandeep et al. 2014). In potato (Solanum tuberosum L.), manure and compost were recorded to improve tuber yield (El-Sayed et al. 2015; Ninh et al. 2015).
The number of SRs varies greatly between individual plants, leading to variation of yield up to 50% (Villordon et al. 2009). Obviously, SR yield of the crop is determined by the plant density, SR number and SR size. The density is adjusted well by commercial practices (Meyers et al. 2014). As the initiation process of SRs determines the number as well as the size and shape of SRs, it is one of the key factors that affect crop yield and market value.
The application of organic amendments was investigated in some previous studies on sweetpotato. Some products such as wheat straw biochar, green leaf manure and poultry manure have been demonstrated to improve SR yield (Agyarko et al. 2013; Liu et al. 2014; Nedunchezhiyan et al. 2010). Applications of organic amendments could stimulate SR development (Isobe et al. 1996) or increase sugar content as well as SR appearance quality (Dou et al. 2012). However, none of these studies focused on the influence of these substances on the storage root formation.
Locally available organic amendments were chosen for this study to examine the effects of these products on sweetpotato SR initiation. Queensland is the biggest producer of sugarcane in Australia which accounts for around 95% of Australia’s sugarcane (SRA 2019) and produces a large amount of trash (10–15 Mg/ha/year) (Zafar 2022). National poultry litter was estimated at around 1.2 million tons per year and expected to increase with industry expansions (McGahan et al. 2013). Obviously, organic amendments are a source of N and available N released into the soil after amending with these products may differ due to amendment properties and application rates. In the previous study, insufficient or excessive N supply inhibited the formation of SRs of ‘Orleans’ sweetpotato cultivar (Dong et al. 2022). Therefore, in this study, we focused on how N soil availability change caused by soil amendments affects SR formation. Accordingly, the study aims (1) to investigate the influence of organic amendments on sweetpotato during SR initiation (2) to determine the rate and type of amendments should be used for sweetpotato cropping.
Materials and methods
Plant materials and growth conditions
The experiment was conducted in a glasshouse at the Queensland Department of Agriculture and Fisheries Bundaberg Research Facility (lat. 24o50′54′ S, long. 152o24′14′ E) from 12 May to 1 July 2019. The average daily maximum and minimum temperature during the study period inside the glasshouse were 26.5 °C and 14.3 °C, respectively, and the average daily maximum and minimum relative humidity were 87.9% and 48.8%, respectively. The average photosynthetic photon flux density inside the glasshouse was 530 µmol m−2 s−1 at midday during the experimental period. Approximate day length is about 10 h 40 min. A sandy loam soil (Dermosol) (Isbell 2016) was used for the experiment. Poultry manure blend (PM) (Fine farm organics, Victoria, Australia) and sugar cane trash (SCT) (ORECO Group, Queensland, Australia) were used as soil amendments in this study. Granular fertilisers including urea (Richgro, Jandakot, West Australia) (46% N as NH4+), Nitrocal (Campbells Fertiliser Australia, Laverton North, Victoria) (15.5% N as NO3−), Potash (Richgro, Jandakot, West Australia) (41.5% K) and superphosphate (Richgro, Jandakot, West Australia) (9.1% P) were added into the soil.
Healthy uniform cuttings of ‘Orleans’ with six fully opened leaves and at least 20 cm long were utilised to examine SR initiation. One cutting was planted horizontally in each pot with three nodes without leaves buried under the sand and three fully opened leave staying above-ground.
Growth medium preparations
Black plastic pots of 20 cm in diameter and 27 cm in height were used in this experiment. 3.5 L of dermosol soil was mixed thoroughly with the respective amount of amendments and fertilizers (Table S1) to fill the pot. Characteristics of soil and organic amendments used for the experiment were analysed at Environmental Analysis Laboratory, Southern Cross University, Australia (Table S2). Available nutrients at the beginning of the experiment were adjusted to the same level in all pots except A0 as N 500 mg, P 1000 mg and K 1800 mg pot−1. The treatment A0 contains N 113 mg, P 42 mg and K 282 mg pot−1. The amount of P and K supplied for plants in this experiment is similar to the total P and K supplied for sweetpotato when using Hoagland’s nutrient solution in previous studies (Dong 2021). N available for each plant was around 50% recommendation for basal N fertilisation of sweetpotato (Loader et al. 1999). Because a key purpose of soil amendment is to increase soil organic carbon ©, amendments were applied to achieve two levels of carbon concentrations in the mixture at 2.16% and 2.55% (from 1.97 of the original soil). Pots were settled in the glasshouse for 1 week before planting. Tap water was added in the pots to field capacity 3 days before transplanting to ensure sufficient and similar moisture in all pots.
Experiment design
The experiment consisted of six treatments including unamended, synthesis fertilisers, PM 22t/ha, PM 66 Mg/ha, SCT 30 Mg/ha and SCT 10 Mg/ha (hereafter A0, AF, PM22, PM66, SCT10 and SCT30, respectively) with multiple harvests over the study period. The pots were arranged in a complete randomized design (CRD). During each harvest, six plants for each treatment were harvested for measurements. Three plants were used for anatomical observations and morphological analysis of the roots, and the other three were used for CN analysis. In total, 4 harvests were conducted over the experiment period including 144 plants (6 treatments × 4 harvests × 6 plants per harvest). Eight additional plants per treatment (48 plants in total) were grown for backup purposes in case death or abnormal growth occurred.
Sampling date
Plants were sampled at 10, 21, 35 and 49 DAT. Six uniform plants divided into two sets were sampled for each treatment on a sampling date. Plants were dug out carefully to minimise root damage and washed in tap water to remove all soil.
Measurements and data collections
Available NH4 +, NO3 − and total C, N in soil samples
Soil samples were taken from three harvested pots for each treatment at planting, 10, 21, 35 and 49 DAT. They were dried in an oven at 40 °C to a constant weight. Then, all samples were stored in the – 80 °C freezer until analysis. A solution of 0.1 M MgSO4 was used to extract NH4+ and NO3− in soil samples (Choosang et al. 2018). The concentration of NH4+ and NO3− were determined by Ammonium and Nitrate Ion Selective Electrodes (ISEs) (Van London Co., Houston, Texas, USA). Total C and N concentration in soil samples were analysed using TruMac® Carbon/Nitrogen Analyser (LECO Corporation, Michigan, USA). C:N ratio was calculated based on their concentration in dried mass basic.
Anatomical changes in ARs
Sampling for root anatomy was performed at 10, 21, 35 and 49 DAT. Three plants from each treatment were dug out carefully and washed in tap water to remove all growth substrates. All adventitious roots (ARs) from an individual plant were harvested and sectioned using sharp razor blades (Villordon et al. 2009). Only very thin sections not cut obliquely were used. Anatomical observations were recorded from sections at around 3–4 cm from the proximal end of roots (Villordon et al. 2009). Transverse sections of all ARs were stained by Toluidine Blue O 0.05% to observe the anatomy under a microscope (Eguchi and Yoshinaga 2008). After staining, they were rinsed in water until there was no stain left on the sections (O’Brien and McCully 1981). Images for each section were taken under a microscope to determine anatomical feature development (Figure S1). Some main anatomical characteristics of ARs were investigated via those images. Other characteristics, including initial development of regular vascular cambium (IRVC), completed regular vascular cambium (RVC), appearance of anomalous cambium (AC) and lignification of more than 50% of the stele cells (LC) were characterised throughout the sampling dates.
Classifying AR development
ARs were classified into storage roots (SRs), pencil roots (PRs) and lignified roots as described by Wilson and Lowe (1973). ARs with anomalous cambium (AC) encircling the primary and secondary xylem elements and/or central metaxylems were considered as SRs (Belehu et al. 2004; Villordon et al. 2009; Wilson and Lowe 1973). Those roots with at least one of the protoxylem elements connected to the central metaxylems by a strand of lignified cells and some meristematic activities were around the central metaxylem developed into PRs. When more than 50% of stele cells was lignified, these roots would be lignified roots. Sections were observed under an Olympus CX31 microscope (Olympus corporation, Tokyo, Japan) and images were taken by a Nikon DS-L2 camera (Nikon corporation, Tokyo, Japan).
Carbon and nitrogen analysis in sweetpotato plants
Three plants from each treatment were harvested at 10, 21, 35 and 49 days after transplanting (DAT). Shoots and roots from each plant were dried separately in an oven at 70 °C to a constant weight. Then, samples were stored in a – 80 °C freezer until analysis. Total N and C in plant samples were analysed using TruMac® Carbon/Nitrogen Analyser (LECO Corporation, Michigan, USA).
Statistical data analysis
All statistical analysis was conducted using IBM® SPSS® software (version 25; IBM, New York, United States) statistical package. Percentage data were transformed using arcsine, whereas measurement data were square root transformed in SPSS for analysis. One-way ANOVA was used to test how plants reacted to different organic soil amendments for each harvest. As different plants were sampled in each harvest, two-way ANOVA (rather than repeated measure ANOVA) was used to analyse the global effects of organic amendments, time and interactive effect of amendment and time on anatomical root features, N acquisition, and the number of initiated SRs. The means were compared using Turkey HSD. P value of less than 0.05 was regarded as statistically significant. Graphs were produced using SigmaPlot® software package (version 14; SYSTAT Software, Inc, California, United States) and Microsoft® Excel® for Office 365 MSO (Microsoft Corporation, Washington, USA).
Results
Effects of soil amendments on N concentration in soil samples
Available N and total N in soil were examined over the study period. Results showed that the effects of soil amendments were statistically different in the concentration of soil available N after planting (Fig. 1). As no N fertilisation was added in A0 treatment, the lowest N availability was recorded in this treatment. Although initial soil available nutrients were modified to the same level in all amended pots, there were significant differences in the concentration of available NO3− and NH4+ among amended treatments due to the different decomposition rate and soil amendment interaction, especially related to NH4+. In the treatments with PM, uric acid in the amendment metabolised rapidly to NH4+, so a higher concentration of NH4+ was recorded in those treatments. In contrast, the immobilisation effect in SCT treatments reduced the available N in soil. Therefore, the highest and lowest available N was observed in the PM66 and SCT30 treatment, respectively. In all observations, both PM treatments had significantly higher available NO3− and NH4+ than treatments with SCT.
There were significant differences in total N in the soil between treatments (Table 1). PM applications increased total N in soil while SCT treatment had trivial effect on the total N due to the low N concentration. Treatment PM66 had significantly higher total N than other treatments on all sampling dates, and reduced C/N ratio in soil, whereas SCT increased this rate (Table 2). The two-way ANOVA results showed that the main effects of amendment treatment and sampling time were significant on the total N and C/N ratio in soil while the interactive effect of treatment by time was not significant. Overall, soil total N reduced over time and C:N ratio increased in all treatments.
Effects of soil amendments on root anatomy of sweetpotato
In the early stage of root development, there was a significant effect of soil amendments on cambium formation. Both treatments with PM increased the rate of roots with NC compared to other treatments at 10 DAT (Table 3). As the activity of primary cambium resulted in the development of IRVC and gradually formed RVC, IRVC reduced over time and could not be found in roots at 49 DAT. Meanwhile, RVC increased during 10 and 21 DAT before decreasing until 49 DAT (Table 3). The appearance of AC was observed in all treatments from day 21 (Table 3). The formation of this characteristic increased until 49 DAT. The treatment PM66 had a significantly lower rate of roots with this feature compared to the control (A0), SCT10 and SCT30 treatments had the highest percentage of root developed AC than other treatments. The development of LC increased over time in all treatments and there was no significant difference among treatments at 21 DAT. After that, the treatment PM66 promoted LC formation with the highest rate observed at both 35 and 49 DAT (Table 3). However, both SCT10 and SCT30 treatments had the lowest rate of lignified roots varying from around 10 to 28%.
Therefore, the formation and development of cambium were all inhibited in both PM22 and PM66 treatments, and lignification was promoted from day 35. In contrast, SCT applications (SCT10 and SCT30 treatments) stimulated the initiation of the vascular cambium and anomalous cambium throughout the SR formation.
Results from this experiment showed that there were significant effects of soil amendment applications on the SR initiation (Fig. 2A). All amended treatments had higher rates of SRs compared to A0. The SCT10 and SCT30 treatments had a similar rate of SRs, which were significantly higher than those of all other treatments. Although AF treatment had a higher distribution of SRs than A0, PM22 and PM66, it was still lower than SCT10 and SCT30 treatments.
In this experiment, there was no statistical difference in the distribution of PRs (Fig. 2B). The percentage of PRs increased over time and reached the highest rate at the final sampling. The main effect of time was significant at different sampling times (P = 0.009).
Effects of soil amendments on plant performances and storage root weight of sweetpotato
Results from this experiment showed that all amended treatments had significantly higher growth parameters above-ground dry weight and root dry weight) compared to A0 (Table 4). PM treatments (PM22 and PM66) increased the above-ground weight of plants but reduced the weight of roots which was mainly due to the reduction in number of SRs. However, SCT application (SCT10 and SCT30 treatments) maintained moderate shoot growth and promoted root weight. Although there was no significant difference among treatments on SR diameter at 49 DAT, a significantly lower value for root length was found in the PM66 treatment. As a result, the lowest SR weight was observed in PM66 treatment, which was similar to that of A0. Treatments SCT10 and SCT30 had the greatest SR weight compared to other treatments.
Effects of soil amendments on N acquisition in plants
Soil amendments had significant effects on N acquisition in plant tissues (Fig. 3A, B). Overall, the concentration of N in vines and roots decreased over time due to the growth of the plants. Higher N concentration in both vines and roots was observed in treatment amended with soil amendments. PM treatments had significantly higher N concentration in both vines and roots and PM66 treatment had the highest concentration of N on all sampling dates. SCT treatments had lower N accumulation in plants and the higher rate SCT application had a lower N concentration in plants.
There was an increasing trend on the C/N ratio in vines and roots (Fig. 3C, D). All treatments with soil amendments had a significantly lower value than unamended treatment (A0). Among amended treatments, the higher ratio was recorded in treatments with SCT, while lower rate was recorded in treatments with PM.
The total N acquisition in plants increased over time in all treatments (Fig. 4). The significantly lower N was observed in treatment A0 on all sampling dates and the highest was found in the PM66 treatment in all observations except 21 DAT. In general, more N was allocated to vines rather than roots, especially for PM66.
Discussion
Effect of soil amendment on available N concentration in soil samples
The two types of soil amendments in our study strongly affected soil available N. Although all treatments except A0 were adjusted to the same soil available N level before the experiment, the application of PM increased the total content of N and the higher rate of PM application led to the higher available N content in soil due to the rapid decomposition of PM. The N soil availability in the PM treatments was mainly NH4+, with a high rate observed right after application until 6 weeks and the availability decreasing with time (Hue and Silva 2000). In line with our results, the use of PM as soil amendments have been recorded to improve N available in soil in previous studies (Agyarko et al. 2013; Hirzel et al. 2007; Ojeniyi et al. 2012). In contrast, SCT applications lowered these parameters compared to PM treatments and AF treatments (unamended with the same level of fertilisation addition). Although SCT amendment slightly increased the total N in soil, it decreased the available N. In line with this result, SCT application at the rate of 3 Mg carbon ha−1 reduced the available NO3−, NH4+ in soil compared to the unamended treatment (Muhammad et al. 2011). It has been suggested that plant residues with C:N ratio higher than 30 are expected to result in immobilisation, while those with C:N ratios less than 30 cause N mineralisation (Alexander 1977). This explains why the concentration of available NO3−, NH4+ in soil responded differently to different type of organic amendments in our experiment. As the available N in the SCT treatments decreased over the study period and the crop required more N for their development, plants in the SCT30 treatments developed some N deficiency symptom after 35 days from the planting. Therefore, addition of N for sweetpotato grown in amended soil needs to be considered to achieve optimum N concentration for SR initiation.
Effect of organic soil amendment on root anatomy of sweetpotato
In this experiment, soil amendments had a significant effect on the formation of sweetpotato SRs, which is likely to be mediated by soil N level. SCT10 treatment promoted the initiation of IRVC and RVC in the early stage of root development as indicated by higher rates of roots with these features at 10 DAT and lowest percentage of roots without cambium development during this period. After that, this treatment continued to promote the formation of AC, and resulted in the highest rate of SR. The treatment SCT30 showed a similar pattern but weaker effect as SCT10. This may be due to a stronger N immobilisation effect, which led to some degree of N deficiency. Similarly, crop residue additions have been recorded to increase the number of tuber in potato (Verma et al. 2011). PM application at either rate (PM22 and PM66 treatments) inhibited the formation of cambium as indicated by the highest rate of NC development in roots. In the later stages, those PM treatments promoted the lignification in roots and reduced the rate of SR in plants. This might be due to the high concentration of N in soil. Overall, the effect of soil organic amendments on the initiation of SR in this study is consistent with SR formation pattern in respose to N fertilisation rates (Dong 2021). The optimal available N level stimulating SR formation in the N rate experiment was 320 mg/pot and 640 mg/pot showed inhibition. This work used 500 mg/pot, which may lead so mile inhibition effect. When SCT N immobilisation brings it down, it shows good stimulation, while decomposition of PM increases the level and lead to inhibition. Therefore, the effect of soil amendments on SR formation is at least partially mediated by the change of soil available N due to soil organic amendment. The results suggested that using organic soil amendments could lead to positive or negative effects on SR initiation of sweetpotato depending on the product’s properties.
According to the results from this study, possible mechanisms related to the formation and development of vascular cambium would associate with the nutrients in the organic amendments added into soil. PM usually contains high amounts of N especially in the form of uric acid (60–70%) (Hue and Silva 2000; Nahm 2003) and the aerobic decomposition of uric acid after amending leads to the high concentration of NH4+ in soil (Nahm 2003; Vogels and Van der Drift 1976). Therefore, application of PM may result in increasing the concentration of available N in soil, which, if accumulated to excessive level, was demonstrated to adversely affect the initiation of SRs in previous studies (Gifford et al. 2008; Togari 1950). Although, nutrients in all pots were adjusted to the same level during amending, SCT pots may have lower N concentrations due to the immobilisation of N in conjunction with the decomposition of this amendment. This could result in the optimal soil available N concentration for SR initiation as indicated by higher rate of initiated SRs compared to other treatments (initial N level was higher than the optimal level for SR formation observed in our previous studies). Another possible reason for the increase of SR formation in SCT treatments would be due to its effect on the bulk density of the soil. It has been suggested that high soil bulk density which is often related to soil compaction, reduced the initiation of SRs (Chua and Kays 1982).
Effects of organic soil amendments on plant performance and yield of Orleans during SR formation stage
Both treatments PM22 and PM66 had significantly higher above-ground biomass but lower root biomass at 49 DAT compared to A0 and AF treatments. This means that those treatments promoted shoot growth but inhibited root growth, leading to reduced SR yield. The growth of vines in sweetpotato is positively related to N fertilisation supply (Knavel 1971) and higher plant biomass is also due to the high nutrient content in soil (Brouwer 1962; Grechi et al. 2007). PM used for the current study had ammoniacal N at a rate of 1880 mg kg−1 and the total N was 3.99%. Therefore, high N content in PM resulted in the promotion of shoot growth in this experiment.
Treatments SCT10 and SCT30 maintained shoot growth and promoted root growth in this pot experiment and thereby moderate above-ground dried biomass and higher root dried biomass were observed in treatments with SCT. The result could be associated with a very high C:N ratio in the SCT amendment. The activity of soil microorganisms to decompose the material added into soil would slowly release N for plants (Hue and Silva 2000). Thus, the available N in soil in the SCT treatments was lower in those treatments compared to PM treatments. In addition, SCT10 had higher available N concentration in soil compared to SCT30, which also resulted in greater growth of both roots and vines.
The SCT applications in this study increased the SR weight of sweetpotato at 49 DAT. Both SCT treatments, SCT10 and SCT30, had significantly higher SR weight compared to all other amendment treatments including AF treatment. This could be due to the improvement of soil properties. A significantly higher C:N ratio in soil was observed in the treatments with SCT in this pot experiment. Soil C:N ratio is important as it affects soil microbial communities, which are a critical soil functional component. Generally, their alteration could affect the nutrient cycle and soil fertility. Plant residue applications have been reported to reduce the bulk density of soil (Zebarth et al. 1999), which was considered to affect SR initiation and development (Chua and Kays 1982; Togari 1950). The application of crop straw at a rate of 7.5 Mg ha−1 has been reported to improve sweetpotato yield in a previous study (Pan et al. 2019). Hence, our result confirmed the positive effects of plant residues on the yield of sweetpotato.
Effects of organic soil amendments application on N accumulation in plants during SR formation stage
By the end of our experiment, the plant tissue N concentration was the highest in PM66 while lowest in SCT30. A possible reason for this would be the N availability in soil and N uptake. It was concluded that the concentration of N in plants is determined by N uptake and depends on the available N in soil (Pederson et al. 2002). Poultry manure amendment has been recorded to increase N uptake in sweetpotato (Siose et al. 2018), and we see a positive correlation between tissue N concentration and soil available N.
On the final harvesting, the accumulation of N in plant of PM 66 treatment was significantly higher than other treatments and allocated mainly in vines. The SCT30 treatment had lower N accumulated in the whole plant compared to others except for the A0 treatment. Statically significant higher N accumulation in potato was observed in treatment amended with PM at rates of 300–600 kg total N ha−1 (Lynch et al. 2008). Similarly, PM application for cassava also resulted in higher N accumulation in plants compared to the control and NPK fertiliser treatments (Ojeniyi et al. 2012). In addition, organic amendments have been reported to affect N uptake of plants. For example, crop residue application at a rate of 10 Mg ha−1 significantly reduced N uptake in corn plants (Nguyen et al. 2016). Therefore, different organic amendments differentially affected N accumulation in plants dependent on the types and application rates.
The accumulation of N in plants is related to N levels supplied in soil. In the PM66 treatment, uric acid in the amendment metabolised rapidly to NH4+ and so higher concentration of NH4+ was found in soil. As a result, higher N accumulation was observed in this treatment as increasing N supply led to higher N accumulation in plants (Pederson et al. 2002). Nitrogen stimulated leaf growth through the synthesis of proteins involved in cell expansion and cytoskeleton synthesis (Bassi et al. 2018). The content of N in leave affected photosynthesis (Allison et al. 1997) as it is partly related to photosynthetic enzymes, pigment content and the size, number and composition of chloroplast. Sufficient N supply leads to easy assimilation which need to be balance by more carbon (Lawlor 2002). Nitrogen is assimilated in roots but the larger part is translocated to leaves which are a sink for N during vegetative stage (Xu et al. 2012). It is also related to allocation. In general, when soil N level is high, plants do not need many roots to absorb resources from soil. In this case, biomass mainly allocate to shoots, leading to high shoot to root ratio, as well as a balance between photosynthates produced by leaves and nutrients absorbed by root. Moreover, some plant hormones such as cytokinin, auxin are associated to N levels (Kiba et al. 2011; Matsumoto-Kitano et al. 2008; Samuelson et al. 1992). These phytohormones stimulate cell growth and plant growth. Therefore, the accumulation and allocation of N were also related to the plant physiological processes such as photosynthesis and plant hormone functions.
Conclusions
Results from this study indicated that the application of soil amendments had significant effects on SR initiation of sweetpotato due to their effects on N availability in soil. Both PM treatments increased N availability in soil, which resulted in suppression of SR formation and promotion of lignified roots. PM66 treatment promoted shoot growth but inhibited root growth, resulting in the highest N accumulation in vines, but the lowest N accumulation in roots. Overdosage of PM negatively impacted FSRW mainly by reducing SR number but the growth of individual SR was not impacted. However, SCT application reduced available N in soil, which is associated with increasing vascular cambium development and SR initiation. Generally, both low and high rates of SCT treatments promoted the formation of SR as indicated by a significantly higher rate of SR at all sampling times compared to other treatments. In addition, those treatments maintained shoot growth and enhanced the growth of roots as suggested by the moderate N accumulation in plants. Our results suggested that only a low rate of PM could be applied to sweetpotato and planting should be delayed from 2–3 weeks after amending to avoid the detrimental effect on SR initiation. A low rate of SCT could be used to promote SR initiation but a high rate of this carbon-rich amendment could lead N deficiency, probably due to N immobilisation.
Author contributions statement
Manuscript title: Sweetpotato storage root initiation as affected by soil organic amendment applications. All persons who meet authorship criteria are listed as authors and all authors certify that we have participated in the study with different roles as below. Hong Tham Dong: conceptualisation, methodology, investigation, formal analysis, writing original draft. Yujuan Li: conceptualisation, review and editing, resources. Philip Brown: conceptualisation, review and editing. Cheng-Yuan Xu: conceptualisation, supervision, methodology, visualization.
Data availability
Authors confirm that all data supporting the findings of the study are included in the manuscript. Raw data that support the findings of the current study are available from the corresponding author on reasonable request.
References
Adekiya A, Agbede T, Aboyeji C, Dunsin O, Simeon V (2019) Effects of biochar and poultry manure on soil characteristics and the yield of radish. Sci Hortic 243:457–463
Agyarko K, Dapaah H, Buah S, Frimpong K (2013) Sweet potato (Ipomoea batatas) yield parameters, soil chemical properties and cost benefit ratios following incorporation of poultry manure and inorganic NPK fertilizers in low nutrient Ghanaian soils. Int J Plant Soil Sci 3(2):129–138
Alexander M (1977) Introduction to soil microbiology. In: Alexander M (ed) Introduction to soil microbiology. Wiley, pp 136–247
Allison J, Williams H, Pammenter N (1997) Effect of specific leaf nitrogen content on photosynthesis of sugarcane. Ann Appl Biol 131(2):339–350
Backer RG, Saeed W, Seguin P, Smith DL (2017) Root traits and nitrogen fertilizer recovery efficiency of corn grown in biochar-amended soil under greenhouse conditions. Plant Soil 415(1):465–477
Bassi D, Menossi M, Mattiello L (2018) Nitrogen supply influences photosynthesis establishment along the sugarcane leaf. Sci Rep 8(1):1–13
Belehu T, Hammes P, Robbertse P (2004) The origin and structure of adventitious roots in sweet potato (Ipomoea batatas). Aust J Bot 52(4):551–558
Brouwer R (1962) Nutritive influences on the distribution of dry matter in the plant. Neth J Agric Sci 10:399–408
Choosang J, Numnuam A, Thavarungkul P, Kanatharana P, Radu T, Ullah S, Radu AJS (2018) Simultaneous detection of ammonium and nitrate in environmental samples using on ion-selective electrode and comparison with portable colorimetric assays. Sensors 18(10):3555
Chua LK, Kays SJ (1982) Assimilation pattern of 14C-photosynthate in developing sweet potato roots. J Am Soc Hortic Sci 107(5):866–871
Davis JG, Wilson CR (2000).Choosing a soil amendment. Colorado State University Cooperative Extension
Dong TH (2021) Understanding agronomic factors that affect the initiation and development of sweetpotato (Ipomoea batatas (L.) Lam.) - The role of nitrogen fertilisation and organic soil amendments. Central Queensland University
Dong HT, Li Y, Brown P, Xu C-Y (2022) Early nitrogen applications promote the initiation of sweetpotato storage roots. J Agric Sci 14(4):19
Donn S, Wheatley RE, McKenzie BM, Loades KW, Hallett PD (2014) Improved soil fertility from compost amendment increases root growth and reinforcement of surface soil on slopes. Ecol Eng 71:458–465
Dou L, Komatsuzaki M, Nakagawa M (2012) Effects of Biochar, Mokusakueki and Bokashi application on soil nutrients, yields and qualities of sweet potato. Int Res J Agric Sci Soil Sci 2(8):318–327
Eguchi T, Yoshinaga M (2008) Effect of application of sucrose and cytokinin to roots on the formation of tuberous roots in sweetpotato (Ipomoea batatas L. Lam). Plant Roots 2:7–13
El-Sayed SF, Hassan HA, El-Mogy MM (2015) Impact of bio-and organic fertilizers on potato yield, quality and tuber weight loss after harvest. Potato Res 58(1):67–81
Gifford ML, Dean A, Gutierrez RA, Coruzzi GM, Birnbaum KD (2008) Cell-specific nitrogen responses mediate developmental plasticity. Proc Natl Acad Sci 105(2):803–808
Grechi I, Vivin P, Hilbert G, Milin S, Robert T, Gaudillère J-P (2007) Effect of light and nitrogen supply on internal C: N balance and control of root-to-shoot biomass allocation in grapevine. Environ Exp Bot 59(2):139–149
Hirzel J, Walter I, Undurraga P, Cartagena M (2007) Residual effects of poultry litter on silage maize (Zea mays L.) growth and soil properties derived from volcanic ash. Soil Sci Plant Nutr 53(4):480–488
Hue NV, Silva JA (2000) Organic soil amendments for sustainable agriculture: organic sources of nitrogen, phosphorus, and potassium. In: Silva JA, Uchida R (Eds.), Plant nutrient management in Hawaii’s soils, approaches for tropical and subtropical agriculture. College of Tropical Agriculture and Human Resources, University of Hawaii, Manoa
Isbell RF (2016) The Australian soil classification. CSIRO. Retrieved 18/05/2020 from https://www.clw.csiro.au/aclep/asc_re_on_line/de/dermsols.htm
Isobe K, Fujii H, Tsuboki Y (1996) Effect of charcoal on the yield of sweet potato. Japanese J Crop Sci 65(3):453–459
Kiba T, Kudo T, Kojima M, Sakakibara H (2011) Hormonal control of nitrogen acquisition: roles of auxin, abscisic acid, and cytokinin. J Exp Bot 62(4):1399–1409
Knavel DE (1971) The influence of N and K nutrition on vine and root development of the early “Allgold” sweetpotato at early stage of storage root enlargement. J Am Soc Hortic Sci 96(6):718–720
Lawlor DW (2002) Carbon and nitrogen assimilation in relation to yield: mechanisms are the key to understanding production systems. J Exp Bot 53(370):773–787
Lin Y, Santen VE, Watts D (2016) The effect of poultry litter application on agricultural production: A meta-analysis of crop yield, nutrient uptake and soil fertility Annual conference on applied statistic in agriculture, Kansas State University
Liu Z, Chen X, Jing Y, Li Q, Zhang J, Huang Q (2014) Effects of biochar amendment on rapeseed and sweet potato yields and water stable aggregate in upland red soil. CATENA 123:45–51
Loader L, Harper S, Amante V, Jackson K, Buchanan P (1999) Sweet Potato Information Kit. Agrolink, your growing guide for better farming guide. Agrilink Series QAL9911. Department of Primary Industries, Queensland Horticulture Institute
Lynch DH, Zheng Z, Zebarth BJ, Martin RC, Systems F (2008) Organic amendment effects on tuber yield, plant N uptake and soil mineral N under organic potato production. Renew Agric 23(3):250–259
Matsumoto-Kitano M, Kusumoto T, Tarkowski P, Kinoshita-Tsujimura K, Václavíková K, Miyawaki K, Kakimoto T (2008) Cytokinins are central regulators of cambial activity. Proc Natl Acad Sci 105(50):20027–20031
McGahan E, Barker S, Poad G, Wiedemann S, Batstone D (2013). Conversion of waste to energy in the chicken meat industry
Mehdizadeh M, Darbandi EI, Naseri-Rad H, Tobeh A (2013) Growth and yield of tomato (Lycopersicon esculentum Mill.) as influenced by different organic fertilizers. Int J Agron Plant Prod 4(4):734–738
Meyers SL, Arancibia RA, Shankle MW, Main J, Gajanayake BR (2014) Sweetpotato storage root initiation
Muhammad W, Vaughan SM, Dalal RC, Menzies NW (2011) Crop residues and fertilizer nitrogen influence residue decomposition and nitrous oxide emission from a Vertisol. Biol Fertil Soils 47(1):15–23
Nahm KH (2003) Evaluation of the nitrogen content in poultry manure. Worlds Poult Sci J 59(1):77–88
Nedunchezhiyan M, Byju G, Dash S (2010) Effects of organic production of orange fleshed sweet potato (Ipomoea batatas L.) on root yield, quality and soil biological health. Int Res J Plant Sci 1(6):136–143
Nguyen DH, Scheer C, Rowlings DW, Grace PR (2016) Rice husk biochar and crop residue amendment in subtropical cropping soils: effect on biomass production, nitrogen use efficiency and greenhouse gas emissions. Biol Fertil Soils 52(2):261–270
Ninh H, Grandy A, Wickings K, Snapp S, Kirk W, Hao J (2015) Organic amendment effects on potato productivity and quality are related to soil microbial activity. Plant Soil 386(1):223–236
O'Brien TP, McCully ME (1981) The study of plant structure : principles and selected methods. Termarcarphi Pty
Ojeniyi S, Adejoro S, Ikotun O, Amusan O (2012) Soil and plant nutrient composition, growth and yield of cassava as influenced by integrated application of NPK fertilizer and poultry manure. New York Sci J 5(9):62–68
Pan X-Y, Li J-Y, Deng K-Y, Xu R-K, Shen R-F (2019) Four-year effects of soil acidity amelioration on the yields of canola seeds and sweet potato and N fertilizer efficiency in an ultisol. Field Crop Res 237:1–11
Pederson GA, Brink GE, Fairbrother TE (2002) Nutrient uptake in plant parts of sixteen forages fertilized with poultry litter. Agron J 94(4):895–904
Samuelson ME, Eliasson L, Larsson C-M (1992) Nitrate-regulated growth and cytokinin responses in seminal roots of barley. Plant Physiol 98(1):309–315
Sandeep K, Sutanu M, Sanjay K, Singh HD (2014) Efficacy of organic manures on growth and yield of radish (Raphanus sativus L.) cv. Japanese White. Int J Plant Sci 9(1):57–60
Siose TK, Guinto DF, Kader MA (2018) Organic amendments increased sweetpotato (Ipomoea batatas L.) yield in a calcareous sandy soil of Samoa. South Pacific J Natl Appl Sci 36(1):36–45
SRA (2019) SRA performance report 2018/2019. https://www.agrifutures.com.au/farm-diversity/sugarcane/
Togari Y (1950) A study on tuberous root formation in sweet potatoes. Bull Natl Agric Exper Stat 68:1–96
Verma SK, Asati BS, Tamrakar SK, Nanda HC, Gupta CR (2011) Effect of organic components on growth, yield and economic returns in potato. Potato J 38(1)
Villordon AQ, LaBonte DR, Firon N, Kfir Y, Pressman E, Schwartz A (2009) Characterization of adventitious root development in sweetpotato. HortScience 44(3):651–655
Vogels GD, Van der Drift C (1976) Degradation of purines and pyrimidines by microorganisms. Bacteriol Rev 40(2):403
Wilson L, Lowe S (1973) The anatomy of the root system in West Indian sweet potato (Ipomoea batatas (L.) Lam.) cultivars. Ann Botany 37(3):633–643
Xiao Q, Zhu L-X, Zhang H-P, Li X-Y, Shen Y-F, Li S-Q (2016) Soil amendment with biochar increases maize yields in a semi-arid region by improving soil quality and root growth. Crop Pasture Sci 67(5):495–507
Xu G, Fan X, Miller AJ (2012) Plant nitrogen assimilation and use efficiency. Annu Rev Plant Biol 63:153–182
Zafar S (2022) Sugarcan trash as biomass resource. Retrieved 8 July from https://www.bioenergyconsult.com/sugarcane-trash-biomass/
Zebarth B, Neilsen G, Hogue E, Neilsen D (1999) Influence of organic waste amendments on selected soil physical and chemical properties. Can J Soil Sci 79(3):501–504
Acknowledgements
The research was funded through CQUniversity’s Research Higher Degree Candidature Budget and Australian Research Training Program Scholarship. The authors are grateful to Professor Arthur Villordon from the LSU AgCenter Sweet Potato Research Station, USA, for significant contributions and professional advice in the root anatomy study of this project. Thanks are expressed to Queensland Department of Agriculture and Fisheries Bundaberg Research Facility and its staffs for their assistance during the experiment.
Funding
Open Access funding enabled and organized by CAUL and its Member Institutions. The authors declare that the study was funded through CQUniversity’s Research Higher Degree Candidature Budget and Australian Research Training Program Scholarship. The research has received support from Professor Arthur Villordon from the LSU AgCenter Sweetpotato Research Station in term of professional advice in the root anatomy. This work was supported by Queensland Department of Agriculture and Fisheries Bundaberg Research Facility and its staffs for the glasshouse operations.
Author information
Authors and Affiliations
Corresponding author
Additional information
Communicated by F. Araniti.
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary Information
Below is the link to the electronic supplementary material.
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Dong, H.T., Li, Y., Brown, P. et al. Sweetpotato storage root formation as affected by soil organic amendment applications. Acta Physiol Plant 45, 88 (2023). https://doi.org/10.1007/s11738-023-03570-3
Received:
Revised:
Accepted:
Published:
DOI: https://doi.org/10.1007/s11738-023-03570-3