Skip to main content
SpringerLink
Log in

Transcriptome and biochemical response to 60Co gamma radiation exposure on the grafted seedlings of Paeonia suffruticosa

  • Original Article
  • Published:
Acta Physiologiae Plantarum Aims and scope Submit manuscript

Abstract

Tree peony is considered as an important ornamental and medicinal plant in China, and 60Co gamma radiation breeding can be potentially used to improve its quality. In this study, we have analyzed the biochemical characteristics and transcriptomic effects induced by different doses of 60Co gamma radiation on the grafted seedlings to explore the possible mutagenic effects of 60Co gamma radiation on the tree peony. The higher radiation doses were found to be harmful to the seedlings, and LD50 (50% of the lethal value) was 40.30 Gy. 60Co gamma radiation treatment obviously affected the physiological and biochemical states of tree peony. The activity of antioxidant enzymes including superoxide dismutase, peroxidase, and catalase gradually increased after radiation exposure, with maximal at 40 Gy. The total soluble protein content gradually decreased, while the content of proline and malondialdehyde was significantly increased. A total of 77,699 unigenes were identified, of which 399, 1447 and 1928 identified were differentially expressed genes (DEGs) in H0 (control) vs H1 (10 Gy), H0 vs H5 (50 Gy), H1 vs H5, respectively. GO and KEGG results showed that the DEGs were closely related to phenylalanine metabolism, lipid metabolism, carbohydrate metabolism pathways, plant hormone signal transduction and secondary biosynthesis metabolism. A total of 768 different expressed transcription factors were identified, which were mainly distributed in MYB, WRKY, NAC and other families related to plant stress regulation which was up-regulated upon exposure to 50 Gy. This is the first transcriptome-based study to demonstrate significant variations in the expression of the various genes in response to 60Co gamma radiation. The findings of this study could be useful in the development of a mutation breeding program for tree peony.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

Similar content being viewed by others

References

  • Akshatha CKR, Somashekarappa HM, Souframanien J (2013) Effffect of gamma irradiation on germination, growth, and biochemical parameters of Terminalia arjuna Roxb. Radiat Prot Environ 36:38–44

    Article  Google Scholar 

  • Alam MZ, McGee R, Hoque MA, Ahammed GJ, Carpenter-Boggs L (2019) Effect of Arbuscular mycorrhizal fungi, selenium and biochar on photosynthetic pigments and antioxidant enzyme activity under arsenic stress in Mung Bean (Vigna radiata). Front Physiol 10:193

    Article  PubMed  PubMed Central  Google Scholar 

  • Beyaz R (2019) Impact of gamma irradiation pretreatment on the growth of common vetch (Vicia sativa L.) seedlings grown under salt and drought stress. Int J Radiat Biol 96:257–266

    Article  PubMed  CAS  Google Scholar 

  • Beyaz R, Sancak C, Yildiz CÇ, Kuşvuran SŞ, Yildiz M (2016) Physiological responses of the M-1 sainfoin (Onobrychis viciifolia Scop) plants to gamma radiation. Appl Radiat Isotopes 118:73–79

    Article  CAS  Google Scholar 

  • Brown N (2013) Mutagenesis: institution of plant breeding, genetics and genomics. University of Georgia, Athens

    Google Scholar 

  • Chen QQ, Niu FF, Yan JL, Chen BS, Wu FF, Guo XH, Yang B, Jiang YQ (2017) Oilseed rape NAC56 transcription factor modulates reactive oxygen species accumulation and hypersensitive response-like cell death. Physiol Plant 160:209–221

    Article  CAS  PubMed  Google Scholar 

  • Conesa A, Gotz S, Garcia-Gomez JM, Terol J, Talon M, Robles M (2005) Blast2GO: a universal tool for annotation, visualization and analysis in functional genomics research. Bioinformatics 21:3674–3676

    Article  CAS  PubMed  Google Scholar 

  • Dawar S, Zaki MJ, Ikram N, Tariq M (2010) Gamma radiation (60Co) exposure and application of antagonists for the suppression of root rot diseases in Sunflower (Helianthus annus L.) and Mung Bean (Vigna radiata (L.) R. Wilczek). Our Nature 8:26–33

    Article  Google Scholar 

  • Deshmukh PD, Malode SN (2018) Effects of gamma radiation on seed germination, plant survival and growth characteristics in Dianthus caryophyllus var. Chabaud. J Glob Biosci 7:5403–5410

    Google Scholar 

  • Ducat DC, Avelar-Rivas JA, Way JC, Silver PA (2012) Rerouting carbon flux to enhance photosynthetic productivity. Appl Environ Microbiol 78:2660–2668

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Gai SP, Zhang YX, Mu P, Liu CY, Liu S, Dong L, Zheng GS (2012) Transcriptome analysis of tree peony during chilling requirement fulfillment: assembling, annotation and markers discovering. Gene 497:256–262

    Article  CAS  PubMed  Google Scholar 

  • Han ZZ, Ma XY, Wei M, Zhao T, Zhan RT, Chen WW (2018) SSR marker development and intraspecific genetic divergence exploration of Chrysanthemum indicum based on transcriptome analysis. BMC Genom 19:291

    Article  CAS  Google Scholar 

  • Hao Q, Ren HX, Jin Z, Wang LS, Huang SC, Liu ZA, Gao ZM, Shu QY (2016) Overexpression of PSK1, a SKP1-like gene homologue, from Paeonia suffruticosa, confers salinity tolerance in Arabidopsis. Plant Cell Rep 36:151–162

    Article  PubMed  CAS  Google Scholar 

  • Huang CP, Qin NN, Sun L, Yu MY, Hu WZ, Qi ZY (2018) Selenium improves physiological parameters and alleviates oxidative stress in strawberry seedlings under low-temperature stress. Int J Mol Sci 19:1913

    Article  PubMed Central  CAS  Google Scholar 

  • Ji ZJ, Zeng YX, Liang Y, Qian Q, Yang CD (2016) Transcriptomic dissection of the rice-fusarium fujikuroi interaction by RNA-Seq. Euphytica 211:123–137

    Article  CAS  Google Scholar 

  • Kapare V, Satdive R, Fulzele DP, Malpathak N (2017) Impact of gamma irradiation induced variation in cell growth and phytoecdysteroid production in Sesuvium portulacastrum. J Plant Growth Regul 36:919–930

    Article  CAS  Google Scholar 

  • Khan ZH, Kumar B, Dhatterwal P, Mehrotra S, Mehrotra R (2017) Transcriptional regulatory network of cis-regulatory elements (Cres) and transcription factors (Tfs) In plants during abiotic stress. Int J Plant Biol Res 5:1064

    Google Scholar 

  • Klopfenstein DV, Zhang L, Pedersen BS, Fidel R, Alex WV, Aurélien N, Mungall CJ, Yunes JM, Olga B, Mark W (2018) GOATOOLS: a python library for Gene Ontology analyses. Sci Rep 8:10872

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kong X, Tian H, Yu Q, Zhang F, Wang R, Gao S, Xu W, Liu J, Sha NE, Fu C (2018) PHB3 maintains root stem cell niche identity through ROS-responsive AP2/ERF transcription factors in arabidopsis. Cell Rep 22:1350–1363

    Article  CAS  PubMed  Google Scholar 

  • Li SS, Wang LS, Shu QY, Wu J, Chen LG, Shao S, Yin DD (2015) Fatty acid composition of developing tree peony (Paeonia section Moutan DC.) seeds and transcriptome analysis during seed development. BMC Genom 16:208

    Article  CAS  Google Scholar 

  • Li Y, Lu JX, Chang YH, Tang WL, Yang QS (2017) Comparative analysis of tree peony petal development by transcriptome sequencing. Acta Physiol Plant 39:216

    Article  CAS  Google Scholar 

  • Liang H, Liu SH, Ou LY (2001) Preliminary study on radiation tolerance of Ginkgo biloba seedlings. Acta Agric Nucl Sin 15:219–223

    CAS  Google Scholar 

  • Liu LQ, Liu JL, Zhang J, Geng H, Zhang YP, Shen HX, Gao XH, Yao YC (2010) Mutagenic effects on tissue culture plants of ornamental crabapple induced by 60Co-γ irradiation. Sci Agric Sin 43:4255–4264

    Google Scholar 

  • Liu YJ, Yu XF, Feng YM, Zhang C, Wang C, Zeng J, Huang Z, Kang HY, Fan X, Sha LN, Zhang HQ, Zhou YH, Gao SP, Chen QB (2017) Physiological and transcriptome response to cadmium in cosmos (Cosmos bipinnatus Cav.) seedlings. Sci Rep 7:14691

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Liu C, Sun QH, Zhao L, Li ZX, Zheng HP, Zhang JR (2018) Heterologous expression of the transcription factor EsNAC1 in arabidopsis enhances abiotic stress resistance and retards growth by regulating the expression of different target genes. Front Plant Sci 9:1495

    Article  PubMed  PubMed Central  Google Scholar 

  • López-Galiano MJ, González-Hernández AI, Crespo-Salvador O, Rausell C, Real MD, Escamilla M, Camañes G, García-Agustín P, González-Bosch C, García-Robles I (2018) Epigenetic regulation of the expression of WRKY75 transcription factor in response to biotic and abiotic stresses in Solanaceae plants. Plant Cell Rep 37:167–176

    Article  PubMed  CAS  Google Scholar 

  • Lu FH, Yoon MY, Cho YI, Chung JW, Kim KT, Cho MC, Cheong SR, Park YJ (2011) Transcriptome analysis and SNP/SSR marker information of red pepper variety YCM334 and Taean. Sci Hortic 129:38–45

    Article  CAS  Google Scholar 

  • Mariadoss A, Satdive R, Fulzele DP, Ramamoorthy S, Zayed H, Younes S, Rajasekaran C (2020) Enhanced production of anthraquinones by gamma-irradiated cell cultures of Rubia cordifolia in a bioreactor. Ind Crops Prod 145:111987

    Article  CAS  Google Scholar 

  • Mendy B, Ombe MWW, Radakovic ZS, Holbein J, Siddique S (2017) Arabidopsis leucine-rich repeat receptor-like kinase NILR1 is required for induction of innate immunity to parasitic nema todes. PLoS Pathog 13:e1006284

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Nakatsuka T, Abe Y, Kakizaki Y, Yamamura S, Nishihara M (2007) Production of red-flowered plants by genetic engineering of multiple flavonoid biosynthetic genes. Plant Cell Rep 26:1951–1959

    Article  CAS  PubMed  Google Scholar 

  • Nazir MB, Mohamad O, Affida AA, Sakinah A (1998) Research highlights on the use of induced mutations for plant improvement in Malaysia. Malay Inst Nucl Technol Res (MINT), Bangi

  • Parchin RA, Ghomi AAN, Badi HN, Eskandari A, Navabpour S, Mehrafarin A (2019) Growth characteristics and phytochemical responses of Iranian fenugreek (Trigonella foenum-graecum L.) exposed to gamma irradiation. Ind Crops Prod 139:111593

    Article  CAS  Google Scholar 

  • Persak H, Pitzschke A (2014) Dominant repression by arabidopsis transcription factor MYB44 causes oxidative damage and hypersensitivity to abiotic stress. Int J Mol Sci 15:2517

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Ramesh HL, Murthy Y, Munirajappa VN (2012) Effect of gamma radiation on morphological and growth parameters of Mulberry variety M5. Int J Sci Nat 3:447–452

    Google Scholar 

  • Sarker S, Rahman MS, Islam MR, Hasna S, Islam MS (2014) Effect of gamma radiation on morpho-physiological characters of soybean. J Environ Sci Nat Resour 7:25–30

    Google Scholar 

  • Shah K, Kumar RG, Verma S, Dubey RS (2001) Effect of cadmium on lipid peroxidation, superoxide anion generation and activities of antioxidant enzymes in growing rice seedlings. Plant Sci 161:1135–1144

    Article  CAS  Google Scholar 

  • Sheppard SC, Evenden WG (1986) Factors controlling the response of fifield crops to very low doses of gamma irradiation of the seed. Can J Plant Sci 66:431–441

    Article  Google Scholar 

  • Shi J, Dong PH, Zhang SL, Huo ZP, Yang JX (2010) Effect of 60Co-radiation on seed radiation dose of peony and herbaceous peony. J Henan Univ Sci Technol Nat Sci 3:72–74

    Google Scholar 

  • Smith GF, Kersten H (1942) Auxin and calines in seedlings from x-rayed seeds. Am J Bot 29:785–819

    Article  CAS  Google Scholar 

  • Song IJ, Kang HG, Kang JY, Kim HD, Bae TW, Kang SY, Lim PO, Adachi T, Lee HY (2009) Breeding of four-leaf white clover (Trifolium repens L.) through 60Co gamma-ray irradiation. Plant Biotechnol Rep 3:191–197

    Article  Google Scholar 

  • Su MH, Zhao LY, Fang T (2012) Effect of 60Co-radiation on germination rate and seedling growth of peony seed. J Agron 3:18–20

    Google Scholar 

  • Surakshitha NC, Soorianathasundaram K, Meenakshi ganesan N (2017) Determination of mutagenic sensitivity of hardwood cuttings of grapes ‘Red Globe’ and ‘Muscat’ (Vitis vinifera L.) to gamma rays. Sci Hortic 226:152–156

    Article  CAS  Google Scholar 

  • Thimm O, Blsing O, Gibon Y, Nagel A, Meyer S, Krüger P, Selbig J, Müller LA, Rhee SY, Stitt M (2010) Mapman: a user-driven tool to display genomics data sets onto diagrams of metabolic pathways and other biological processes. Plant J 37:914–939

    Article  CAS  Google Scholar 

  • Wu HB, Gong H, Liu P, He XL, Luo SB, Zheng XM, Zhang CY, He XM, Luo JN (2014) Large-scale development of EST-SSR markers in sponge gourd via transcriptome sequencing. Mol Breed 34:1903–1915

    Article  CAS  Google Scholar 

  • Wu JH, Zhang J, Lan F, Fan WF, Li W (2020) Morphological, cytological, and molecular variations induced by gamma rays in ground-grown chrysanthemum ‘Pinkling.’ Can J Plant Sci 100:68–77

    Article  CAS  Google Scholar 

  • Xie C, Mao X, Huang J, Ding Y, Wu J, Dong S, Kong L, Gao G, Li CY, Wei L (2011) KOBAS 2.0: a web server for annotation and identification of enriched pathways and diseases. Nucleic Acids Res 39:316–322

    Article  CAS  Google Scholar 

  • Yan YH, Zhu YW, Wei WQ, Xu YC (2017) Effects of different doses of 60Co-radiation on rooting, seedling emergence and seedling growth of Paeonia suffruticosa ‘Fengdan’ seeds. J Jiangsu Fores Sci Technol 44:17–21

    Google Scholar 

  • Zhang C, Wang WN, Wang YJ, Gao SL, Du DN, Fu JX, Dong L (2014) Anthocyanin biosynthesis and accumulation in developing flowers of tree peony (Paeonia suffruticosa) ‘Luoyang Hong’. Postharvest Biol Technol 97:11–22

    Article  CAS  Google Scholar 

  • Zhang YZ, Cheng YW, Ya HY, Xu SZ, Han JM (2015) Transcriptome sequencing of purple petal spot region in tree peony reveals differentially expressed anthocyanin structural genes. Front Plant Sci 6:964

    PubMed  PubMed Central  Google Scholar 

  • Zhang J, Kevin K, Bryan UJ, John E, Gregory MD (2017) Changes of leaf membrane fatty acid composition and saturation level of warm-season turfgrass during drought stress. Crop Sci 57:2843

    Article  CAS  Google Scholar 

  • Zhao YL, Li CC, Chen X (2012) Effect of 60Co-γ radiation on the dwarfing mechanism of Rhododendron. N Gard 9:48–50

    CAS  Google Scholar 

  • Zhou S, Zou X, Zhou Z, Liu J, Xu C, Yu J et al (2014) Multiple species of wild tree peonies gave rise to the ‘king of flflowers’, Paeonia suffruticosa Andrews. Proc Biol Sci 281:1687–1694

    Google Scholar 

Download references

Acknowledgements

This work was supported by the Strategic Priority Research Program of the Chinese Academy of Sciences (no. XDA23080601), National Natural Science Foundation of China (no. 31601789) and the Foundation of High-level Talents of Qingdao Agricultural University (6631114306).

Author information

Authors and Affiliations

  1. College of Landscape Architecture and Forestry, Qingdao Agricultural University, Qingdao, 266109, China

    Pengyao Wang, Dandan Geng, Shufan Song, Kuiling Wang & Qing Hao

  2. Shandong Radiation Center, Shandong Academy of Agricultural Science, Jinan, 250000, China

    Zhaohua Wang

Authors
  1. Pengyao Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Dandan Geng
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Shufan Song
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Zhaohua Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Kuiling Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Qing Hao
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Qing Hao.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Additional information

Communicated by R. Beckett.

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

Rights and permissions

Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Wang, P., Geng, D., Song, S. et al. Transcriptome and biochemical response to 60Co gamma radiation exposure on the grafted seedlings of Paeonia suffruticosa. Acta Physiol Plant 44, 91 (2022). https://doi.org/10.1007/s11738-022-03426-2

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s11738-022-03426-2

Keywords

Search

Navigation