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Effect of salt stress on seed germination, morphology, biochemical parameters, genomic template stability, and bioactive constituents of Andrographis paniculata Nees

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Abstract

High level of salinity present in the soil severely affects plant growth and metabolism, eventually reduces crop productivity. In the present study, we have made an effort to obtain detailed insight on the effect of various levels of salinity on various physiological, biochemical, genetic, and phytochemical parameters of Andrographis paniculata genotype, CIM-Megha, in an attempt towards development of a salt-tolerant variety. The results showed that maximum seed germination efficiency was observed at 100 mM among the various salt concentrations. Moreover, with the increase in salt concentration, the overall growth of the plant was stunted. High salinity had a negative effect on photosynthetic pigments, free cysteine content, non-protein thiol content, and nitrate reductase activity. However, proline accumulation and phenol content were found to increase with the increasing salt concentration. The results from the study demonstrated that activities of CAT and APX antioxidant enzymes increased with the applied salt stress. The accumulation of reactive oxygen species in response to salinity is the most important DNA-damaging factor causing a decrease in the genomic template stability of the plant. Quantification of important bioactive constituents (andrographolide, neo-andrographolide, and 14-DDA) was done through HPLC, and the results showed high variability in constituents. In summary, Andrographis paniculata could be grown at large scale in saline areas having up to 100 mM salt concentration.

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Acknowledgments

We express our sincere thanks to Director, CSIR-CIMAP, Lucknow, for his continuous support and providing the laboratory facilities required to carry out this research (CIMAP Institutional Communication No.: CIMAP/PUB/2018/79). The financial support from the Council of Scientific and Industrial Research (CSIR), New Delhi through the Mission Programme “Phytopharmaceutical Mission—(HCP-010)” is gratefully acknowledged. Financial assistance in the form of Senior Research Fellowship to AK from Indian Council of Medical Research (ICMR), New Delhi (No.: 45/28/2018/TM/BMS) is also gratefully acknowledged.

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  1. CSIR-Central Institute of Medicinal and Aromatic Plants, Research Centre, Bengaluru, 560 065, India

    Amit Kumar, Vereena Rodrigues, Sanchi Verma, Channaya Hiremath & Velusamy Sundaresan

  2. CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, 226 015, India

    Manju Singh, Karuna Shanker & Ashutosh K. Shukla

  3. Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201 002, India

    Amit Kumar, Vereena Rodrigues, Karuna Shanker, Ashutosh K. Shukla & Velusamy Sundaresan

  4. Phytopharmaceuticals Division, Indian Pharmacopoeia Commission, Ghaziabad, 201 002, India

    Amit Kumar

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Kumar, A., Rodrigues, V., Verma, S. et al. Effect of salt stress on seed germination, morphology, biochemical parameters, genomic template stability, and bioactive constituents of Andrographis paniculata Nees. Acta Physiol Plant 43, 68 (2021). https://doi.org/10.1007/s11738-021-03237-x

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