Abstract
Nitrogen is necessary to synthesize compounds such as chlorophyll, amino acids, nucleic acids, proteins, lipids, and other nitrogen (N) metabolites. In this sense, saline stress produces a decrement in the quality and quantity of crop production around the world due to an osmotic and ionic imbalance that alters the N metabolism. The objective of this work is to verify if the genotypic variability and a better nitrogen metabolism regulation improve tolerance to saline stress in tomato plants. This study was conducted with (Grand Brix and Marmande RAF) two tomato commercial genotypes (Solanum lycopersicum L). N forms, N metabolism, N use efficiency (NUE) parameters and amino acid profile were analyzed. A greater GS/GOGAT cycle enzyme activity could promote a better N integration in the plant, besides it promotes the generation of osmoprotective amino acids such as proline and improves the salt stress tolerance. A more effective N metabolism regulation indicates more salt tolerance. Our results showed a better effective N metabolism regulation by Grand Brix.
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References
Abenavoli MR, Longo C, Lupini A, Miller AJ, Araniti F, Mercati F, Princi MP, Sunseri F (2016) Phenotyping two tomato genotypes with different nitrogen use efficiency. Plant Physiol Biochem 107:21–32
Ashraf M, Shahzad SM, Imtiaz M, Rizwan MS, Arif MS, Kausar R (2018) Nitrogen nutrition and adaptation of glycophytes to saline environment: a review. Arch Agron Soil Sci 64:1181–1206. https://doi.org/10.1080/03650340.2017.1419571
Baethgen WE, Alley MM (1989) A manual colorimetric procedure for measuring ammonium nitrogen in soil and plant Kjeldahl digests. Commun Soil Sci Plant Anal 20:961–969. https://doi.org/10.1080/00103628909368129
Bieleski RL, Turner NA (1966) Separation and estimation of amino acids in crude plant extracts by thin-layer electrophoresis and chromatography. Anal Biochem 17:278–293
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
Cataldo DA, Maroon M, Schrader LE, Youngs VL (1975) Rapid colorimetric determination of nitrate in plant tissue by nitration of salicylic acid. Commun Soil Sci Plant Anal 6:71–80
Causin HF (1996) The central role of amino acids on nitrogen utilization and plant growth. J Plant Physiol 149:358–362
De la Torre-Gonzalez A, Montesinos-Pereira D, Blasco B, Ruiz JM (2018) Influence of the proline metabolism and glycine betaine on tolerance to salt stress in tomato (Solanum lycopersicum L.) commercial genotypes. J Plant Physiol 231:329–336
De la Torre-González A, Navarro-León E, Albacete A, Blasco B, Ruiz JM (2017) Study of phytohormone profile and oxidative metabolism as key process to identification of salinity response in tomato commercial genotypes. J Plant Physiol 216:164–173
Debouba M, Gouia H, Suzuki A, Ghorbel MH (2006) NaCl stress effects on enzymes involved in nitrogen assimilation pathway in tomato “Lycopersicon esculentum” seedlings. J Plant Physiol 163:1247–1258
Debouba M, Maâroufi-Dghimi H, Suzuki A, Ghorbel MH, Gouia H (2007) Changes in growth and activity of enzymes involved in nitrate reduction and ammonium assimilation in tomato seedlings in response to NaCl stress. Ann Bot 99:1143–1151
Elliot GC, Läuchli A (1985) Phosphorous efficiency and phosphate–iron interactions in maize. Agron J 77:399–403
Feierabend J, Beevers H (1972) Developmental studies on microbodies in wheat leaves. Planta 123:63–77
Galili G, Amir R, Fernie AR (2016) The regulation of essential amino acid synthesis and accumulation in plants. Annu Rev Plant Biol 67:153–178
Ghanem ME, Martínez-Andújar C, Albacete A, Pospíšilová H, Dodd IC, Pérez-Alfocea F, Lutts S (2011) Nitrogen form alters hormonal balance in salt-treated tomato (Solanum lycopersicum L.). J Plant Growth Regul 30:144–157
Gonzalez EM, Gordon AJ, James CL, Arrese-Igor C (1995) The role of sucrose synthase in the response of soybean nodules to drought. J Exp Bot 26:1515–1523
Groat RG, Vance CP (1981) Root nodule enzymes of ammonia assimilation in alfalfa (Medicago sativa L.). Developmental patterns and response to applied nitrogen. Plant Physiol 67:1198–1203
Hageman RH, Hucklesby DP (1971) Nitrate reductase. Meth Enzym 23:497–503
Hildebrandt TM, Nesi AN, Araújo WL, Braun HP (2015) Amino acid catabolism in plants. Mol Plant 8:1563–1579
Holder M, Rej R, Berjmeyer HU, Bergmeyer J (1983) Alanine aminotransferase. In: Bergmeyer HU, Bergmeyer I, Grassl M (eds) Methods of enzymatic analysis, 3rd edn. Verlag-Chemie, Weinheim, pp 444–456
Igarashi D, Tsuchida H, Miyao M, Ohsumi C (2006) Glutamate:glyoxylate aminotransferase modulates amino acid content during photorespiration. Plant Physiol 142:901–910
Krom MD (1980) Spectrophotometric determination of ammonia: a study of a modified Berthelot reaction using salicylate and dichloroisocyanurate. Analyst 105:305–316. https://doi.org/10.1039/an9800500305
Lea PJ, Azevedo RA (2007) Nitrogen use efficiency. 2. Amino acid metabolism. Ann Appl Biol 151:269–275
Mishra P, Mishra V, Takabe T, Rai V, Singh NK (2016) Elucidation of salt-tolerance metabolic pathways in contrasting rice genotypes and their segregating progenies. Plant Cell Rep 35:1273–1286
Saito T, Matsukura C, Ban Y, Shoji K, Sugiyama M, Fukuda N, Nishimura S (2008) Salinity stress affects assimilate metabolism at the gene-expression level during fruit development and improves fruit quality in tomato (Solanum lycopersicum L.). J Japan Soc Hort Sci 77:61–68
Sánchez-Rodríguez E, Rubio-Wilhelmi MM, Ríos JJ, Blasco B, Rosales MÁ, Melgarejo R, Romero L, Ruiz JM (2011) Ammonia production and assimilation: its importance as a tolerance mechanism during moderate water deficit in tomato plants. J Plant Physiol 168:816–823
Shao QS, Shu S, Du J, Xing WW, Guo SR, Sun J (2015) Effects of NaCl stress on nitrogen metabolism of cucumber seedlings. Russ J Plant Physiol 62:595–603
Shi-Wei GUO, Yi ZHOU, Ying-Xu GAO, Yong LI, Qi-Rong SHEN (2007) New insights into the nitrogen form effect on photosynthesis and photorespiration. Pedosphere 17:601–610
Siddiqi MY, Glass AD (1981) Utilization index: a modified phosphorous nutrition of eight forms of two clover species, Trifolium ambiguum and Trifolium repens. J Plant Nutr 4:289–302
Singh RP, Srivastava HS (1986) Increase in glutamate synthase (NADH) activity in maize seedlings in response to nitrate and ammonium nitrogen. Physiol Plant 66:413–416
Surabhi GK, Reddy AM, Kumari GJ, Sudhakar C (2008) Modulations in key enzymes of nitrogen metabolism in two high yielding genotypes of mulberry (Morus alba L.) with differential sensitivity to salt stress. Environ Exp Bot 64:171–179
Wallsgrove RM, Lea PJ, Miflin BJ (1979) Distribution of the enzymes of nitrogen assimilation within the pea leaf cell. Plant Physiol 63:232–236
Xu G, Fan X, Miller AJ (2012) Plant nitrogen assimilation and use efficiency. Ann rev plant biol 63:153–182
Zhonghua T, Yanju L, Xiaorui G, Yuangang Z (2011) The combined effects of salinity and nitrogen forms on Catharanthus roseus: the role of internal ammonium and free amino acids during salt stress. J Plant Nutr Soil Sci 174:135–144
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This study was financed by the PAI program (Plan Andaluz de Investigación, Grupo de investigación AGR282).
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de la Torre-González, A., Navarro-León, E., Blasco, B. et al. Nitrogen and photorespiration pathways, salt stress genotypic tolerance effects in tomato plants (Solanum lycopersicum L.). Acta Physiol Plant 42, 2 (2020). https://doi.org/10.1007/s11738-019-2985-8
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DOI: https://doi.org/10.1007/s11738-019-2985-8