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Physiological mechanism of programmed cell death aggravation and acceleration in wheat endosperm cells caused by waterlogging

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Abstract

Wheat (Triticum aestivum L.) is one of the world’s three major food crops, the previous studies have indicated that the development of wheat endosperm cells underwent a special programmed cell death (PCD) and it could be accelerated by waterlogging. Hua 8 wheat cultivar (waterlogging tolerant) and Hua 9 (waterlogging sensitive) were used to reveal the physiological mechanism in this study. The results showed that after 7 days waterlogging treatment (DWT), the malondialdehyde (MDA) and O2 − (superoxide anion-free radical) were increased in both endosperms, especially in Hua 9 endosperm. Content of hydrogen peroxide (H2O2) increased significantly in both endosperms at 12 days after flowering (DAF) and 15 DAF. Proline, soluble sugar, and protein contents in Hua 8 endosperm were increased, nevertheless, the soluble sugar and proline contents declined in Hua 9 endosperm. The activities of superoxide dismutase (SOD) were induced in both endosperms, especially in Hua 8, while showed different change trends in activities of peroxidase (POD) and catalase (CAT). The SOD isozyme spectrogram appeared a new band, and CAT added two bands in Hua 8 endosperm after 7 DWT. CAT isozyme activity was increased markedly, but SOD isozyme in Hua 9 endosperm showed no significant effect by waterlogging stress. Above all, the accumulation of MDA, O2 −, and H2O2 in wheat endosperm after waterlogging led to the aggravation of PCD progress. Compared to Hua 8, more reactive oxygen species (ROS) accumulated in Hua 9 endosperm after waterlogging, combined with the weak ROS scavenging enzyme activities, in addition to the low osmotic pressure accelerated the PCD progress.

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Acknowledgements

This work was supported by the National Natural Foundation of China (Grant Nos. 31171469 and 31471428). The author would like to acknowledge the anonymous reviewers for their valuable comments.

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Authors and Affiliations

  1. Laboratory of Cell Biology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, People’s Republic of China

    Min Yu, Zhuqing Zhou & Yuanhong Qi

  2. College of Food and Life Science and Technology, Wuhan Institute of Design and Sciences, Wuhan, 430070, Hubei, People’s Republic of China

    Xiangyi Deng & Jiwei Li

  3. College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, People’s Republic of China

    Xiangyi Deng

  4. College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, People’s Republic of China

    Fangzhu Mei

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  1. Min Yu
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  2. Zhuqing Zhou
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  3. Xiangyi Deng
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  4. Jiwei Li
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Correspondence to Zhuqing Zhou.

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Communicated by J. Zwiazek.

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Yu, M., Zhou, Z., Deng, X. et al. Physiological mechanism of programmed cell death aggravation and acceleration in wheat endosperm cells caused by waterlogging. Acta Physiol Plant 39, 23 (2017). https://doi.org/10.1007/s11738-016-2324-2

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  • DOI: https://doi.org/10.1007/s11738-016-2324-2

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