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Alterations in metabolite profile and free radical scavenging activities of Withania somnifera leaf and root extracts under supplemental ultraviolet-B radiation

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Abstract

The effects of supplemental ultraviolet-B (s-UV-B; 3.6 kJ m−2 day−1 above ambient) radiation on plant metabolite profile and free radical scavenging activities of Withania somnifera (an indigenous medicinal plant) under field conditions were investigated. The metabolite profiles of both leaves and roots were analyzed via GC–MS. The methanolic extracts were examined for their DPPH radical-, superoxide radical-, hydrogen peroxide-, hydroxyl radical-, and nitric oxide radical scavenging activities, ferrous ion chelating activity, and reducing power. GC–MS profile of leaves revealed increment in compounds such as isophytol (138.1 %), β-stigmasterol (183.05 %), trans-squalene (233.3 %), and Withaferin A (155.0 %), while compounds such as eugenol, β-carotene, lycopene, and vitamin E were detected in s-UV-B-treated leaves only. In roots, compounds such as ledol, neophytadiene, palmitic acid, retinol, sitosteryl oleate, and campesterol registered their presence only under s-UV-B. Methanolic extracts of treated plant organs were found to be more potent as free radical scavengers (their EC50 values being lower than those of control extracts). Anomalies were observed for nitric oxide radical scavenging in both leaves and roots. The present study indicates that s-UV-B alters the composition and contents of plant metabolites leading to an increase in their free radical scavenging activities. Hence, s-UV-B-treated plant organs might be more effective in combating oxidative stress as well as from a nutritional and health perspective.

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Abbreviations

AsA:

Ascorbic acid

BHT:

Butylated hydroxytoluene

DAT:

Days after transplantation

DPPH:

1,1-Diphenyl-2-picrylhydrazyl

EC:

Effective concentration

GC–MS:

Gas chromatography mass spectroscopy

MDA:

Malondialdehyde

ODSs:

Ozone depleting substances

RNS:

Reactive nitrogen species

ROS:

Reactive oxygen species

UV:

Ultraviolet

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Acknowledgments

The authors are thankful to the Head, Department of Botany and Coordinator, Centre of Advanced Study in Botany, Banaras Hindu University, for providing laboratory facilities, and to the University Grants Commission (UGC), New Delhi, for financial assistance. The authors also extend their thanks to Dr. Ajai Kumar, Advanced Instrumentation Research Facility, Jawaharlal Nehru University, New Delhi, for GC–MS analysis.

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  1. Laboratory of Air Pollution and Global Climate Change, Department of Botany, Banaras Hindu University, Varanasi, 221 005, India

    Swabha Takshak & S. B. Agrawal

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  1. Swabha Takshak
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  2. S. B. Agrawal
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Correspondence to S. B. Agrawal.

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Communicated by J. Gao.

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Takshak, S., Agrawal, S.B. Alterations in metabolite profile and free radical scavenging activities of Withania somnifera leaf and root extracts under supplemental ultraviolet-B radiation. Acta Physiol Plant 37, 260 (2015). https://doi.org/10.1007/s11738-015-2014-5

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  • DOI: https://doi.org/10.1007/s11738-015-2014-5

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