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Effect of herbal cake-partitioned moxibustion on MEK1/2 and ERK1/2 expressions of gastric tissues in rats with spleen deficiency syndrome

隔药饼灸对脾虚证大鼠胃组织MEK1/2 及ERK1/2 的影响

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Abstract

Objective

To observe the effect of herbal cake-partitioned moxibustion on the expressions of mitogen-activated protein kinase (MEK1/2) and extracellular regulatory protein kinase (ERK1/2) in gastric tissues of rats with spleen deficiency syndrome, and to explore the possible mechanisms of herbal cake-partitioned moxibustion in treating spleen deficiency syndrome.

Methods

Sixty Sprague-Dawley (SD) rats were randomly divided into a blank control group (group A), a model group (group B), a ranitidine group (group C), and a herbal cake-partitioned moxibustion group (group D) by random digit, 15 rats in each group. Rat models of spleen deficiency syndrome were made by intragastric administration of 4 °C 200% concentrated Da Huang (Radix et Rhizoma Rhei). After successful modeling, the rats in group C were treated with 25 mg/(kg·bw) ranitidine by intragastric adminstration and rats in group D were treated with herbal cake-partitioned moxibustion at Zusanli (ST 36) and Zhongwan (CV 12), for 8 d. Excepted for rats in group A, all the other rats were treated with indomethacin at 5 mg/(kg·bw) at 8:00 a.m. on the second day after finishing all the intervention and sacrificed 7 h later to isolate the stomach. Histopathological changes of the gastric tissues were observed under light microscope after hematoxylin-eosin (HE) staining. The protein expressions of MEK1/2 and ERK1/2 in the gastric tissues were detected by immunohistochemistry.

Results

After intervention, the gastric mucosal injury in group B was significantly severer than that in group A, with large breakage and ablating; the damage of gastric mucosa was decreased in group C compared with group B; the gastric mucosal surface remained relatively complete, and the status of breakage and ablating was significantly improved. After intervention, compared with group A, the protein expressions of MEK1/2 and ERK1/2 in gastric tissues of the other groups were significantly higher (P<0.01). Compared with group B, the protein expressions of MEK1/2 and ERK1/2 in group C and D were significantly higher (all P<0.01). Compared with group C, the protein expressions of MEK1/2 and ERK1/2 in group D were significantly higher (P<0.01).

Conclusion

Herbal cake-partitioned moxibustion promotes the repair of gastric mucosa in rats with spleen deficiency syndrome, via improving protein expressions of MEK1/2 and ERK1/2 in gastric tissues, as well as activating MEK/ERK signaling pathway.

摘要

目的

通过观察隔药饼灸对脾虚证大鼠胃组织分裂原活化蛋白激酶(MEK1/2)及细胞外调节蛋白激酶(ERK1/2) 蛋白表达的影响, 探讨隔药饼灸治疗脾虚证的可能作用机制。

方法

将60 只SPF 级Sprague-Dawley (SD)大鼠按随 机数字表法分为空白对照组(A 组)、模型组(B 组)、雷尼替丁组(C 组)和隔药饼灸组(D 组), 每组15 只。通过200% 的大黄浓缩液4 ℃灌胃制作脾虚证大鼠模型。造模成功后, C 组按25 mg/(kg·bw)灌胃给药, D 组接受隔药饼灸足三 里和中脘治疗, 连续治疗8 d。除A 组外, 其余组大鼠于治疗完成的第二天上午8:00 接受消炎痛5 mg/(kg·bw)灌 胃, 7 h 后处死动物, 取胃。应用苏木精-伊红(HE)染色, 光镜下观察大鼠胃组织病理学改变, 采用免疫组化法检测 大鼠胃组织MEK1/2 及ERK1/2 蛋白表达。

结果

干预结束后, 与A 组相比, B 组大鼠胃黏膜损伤明显, 可见较大的 破损、脱落; 与B 组比较, C 组大鼠胃黏膜表面有部分脱落, 破损情况改善, D 组大鼠胃黏膜表面较完整, 脱落及破 损明显改善。干预结束后, 与A 组比较, 其他各组大鼠胃组织MEK1/2 及ERK1/2 蛋白表达明显升高(均P<0.01); 与B 组比较, C 组和D 组大鼠胃组织MEK1/2 及ERK1/2 蛋白表达升高(均P<0.01); 与C 组比较, D 组胃组织MEK1/2 及ERK1/2 蛋白表达升高(P<0.01)。

结论

隔药饼灸通过提高胃组织MEK1/2 及ERK1/2 蛋白表达, 激活MEK/ERK 信 号转导通路, 进而促进脾虚证大鼠胃黏膜的修复。

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Acknowledgments

This work was supported by National Basic Research Program of China (973 Program, 国家重点基础研究发展 计划, No. 2015CB554502); Fund Project of Hunan Province Education Office (湖南省教育厅科研计划项目, No.13C685); Graduate Student Research Innovation Project of Hunan Province (湖南省研究生科研创新项目, No. CX2016B351).

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  1. Hunan University of Chinese Medicine, Changsha, 410208, China

    Ding-yan Bi  (贲定严), Qiong Liu  (刘琼), Zhan Yi  (易展), Dan Li  (李丹), Yi-fan Zou  (邹逸凡), Ming-zhu Ma  (马明珠), Mi Liu  (刘密) & Xiao-rong Chang  (常小荣)

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  1. Ding-yan Bi  (贲定严)
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  2. Qiong Liu  (刘琼)
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Correspondence to Xiao-rong Chang  (常小荣).

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Bi, Dy., Liu, Q., Yi, Z. et al. Effect of herbal cake-partitioned moxibustion on MEK1/2 and ERK1/2 expressions of gastric tissues in rats with spleen deficiency syndrome. J. Acupunct. Tuina. Sci. 15, 305–310 (2017). https://doi.org/10.1007/s11726-017-1019-6

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