Skip to main content
Log in

Effect of calcium lactate on compressive strength and self-healing of cracks in microbial concrete

  • Research Article
  • Published:
Frontiers of Structural and Civil Engineering Aims and scope Submit manuscript

Abstract

This paper presents the effect on compressive strength and self-healing capability of bacterial concrete with the addition of calcium lactate. Compared to normal concrete, bacterial concrete possesses higher durability and engineering concrete properties. The production of calcium carbonate in bacterial concrete is limited to the calcium content in cement. Hence calcium lactate is externally added to be an additional source of calcium in the concrete. The influence of this addition on compressive strength, self-healing capability of cracks is highlighted in this study. The bacterium used in the study is bacillus subtilis and was added to both spore powder form and culture form to the concrete. Bacillus subtilis spore powder of 2 million cfu/g concentration with 0.5% cement was mixed to concrete. Calcium lactates with concentrations of 0.5%, 1.0%, 1.5%, 2.0%, and 2.5% of cement, was added to the concrete mixes to test the effect on properties of concrete. In other samples, cultured bacillus subtilis with a concentration of 1×105 cells/mL was mixed with concrete, to study the effect of bacteria in the cultured form on the properties of concrete. Cubes of 100 mm×100 mm×100 mm were used for the study. These cubes were tested after a curing period of 7, 14 and 28 d. A maximum of 12% increase in compressive strength was observed with the addition of 0.5% of calcium lactate in concrete. Scanning electron microscope and energy dispersive X-ray spectroscopy examination showed the formation of ettringite in pores; calcium silicate hydrates and calcite which made the concrete denser. A statistical technique was applied to analyze the experimental data of the compressive strengths of cementations materials. Response surface methodology was adopted for optimizing the experimental data. The regression equation was yielded by the application of response surface methodology relating response variables to input parameters. This method aids in predicting the experimental results accurately with an acceptable range of error. Findings of this investigation indicated the influence of added calcium lactate in bio-concrete which is quite impressive for improving the compressive strength and self-healing properties of concrete.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Muhammad N Z, Keyvanfar A, Majid M Z A, Shafaghat A, Mirza J. Waterproof performance of concrete: A critical review on implemented approaches. Construction & Building Materials, 2015, 101: 80–90

    Article  Google Scholar 

  2. Rapoport J, Aldea C M, Shah S P, Ankenman B, Karr A. Permeability of cracked steel fiber-reinforced concrete. Journal of Materials in Civil Engineering, 2002, 14(4): 355–358

    Article  Google Scholar 

  3. Jonkers H, Schlangen E. Development of a bacteria-based self healing concrete. In: Walraven J C, Stoelhorst D, eds. Tailor Made Concrete Structures. London: CRC Press, 2008, 109

    Chapter  Google Scholar 

  4. De Muynck W, De Belie N, Verstraete W. Microbial carbonate precipitation in construction materials: A review. Ecological Engineering, 2010, 36(2): 118–136

    Article  Google Scholar 

  5. Andalib R, Majid M Z A, Hussin M W, Ponraj M, Keyvanfar A, Mirza J, Lee H S. Optimum concentration of Bacillus megaterium for strengthening structural concrete. Construction & Building Materials, 2016, 118: 180–193

    Article  Google Scholar 

  6. Luo M, Qian C X, Li R Y. Factors affecting crack repairing capacity of bacteria-based self-healing concrete. Construction & Building Materials, 2015, 87: 1–7

    Article  Google Scholar 

  7. Siddique R, Nanda V, Kunal E H, Kadri M, Iqbal Khan M, Singh M, Rajor A. Influence of bacteria on compressive strength and permeation properties of concrete made with cement baghouse filter dust. Construction & Building Materials, 2016, 106: 461–469

    Article  Google Scholar 

  8. De Muynck W, Cox K, De Belie N, Verstraete W. Bacterial carbonate precipitation as an alternative surface treatment for concrete. Construction & Building Materials, 2008, 22(5): 875–885

    Article  Google Scholar 

  9. Talaiekhozani A, Keyvanfar A, Andalib R, Samadi M, Shafaghat A, Kamyab H, Majid M Z A, Zin R M, Fulazzaky M A, Lee C T, Hussin M W. Application of Proteus mirabilis and Proteus vulgaris mixture to design self-healing concrete. Desalination and Water Treatment, 2014, 52(19–21): 3623–3630

    Article  Google Scholar 

  10. Jonkers H M, Schlangen E. A two component bacteria-based selfhealing concrete. Cultures, 2009, 215–220

    Google Scholar 

  11. Siddique R, Chahal N K. Effect of ureolytic bacteria on concrete properties. Construction & Building Materials, 2011, 25(10): 3791–3801

    Article  Google Scholar 

  12. Sangadji S, Wiktor V, Jonkers H, Schlangen E. Injecting a liquid bacteria-based repair system to make porous network concrete healed. In: Proceedings of the 4th International Conference on Self- Healing Materials. Ghent, 2013, 118–122

    Google Scholar 

  13. Wiktor V, Jonkers H M. Case studies in construction materials field performance of bacteria-based repair system: Pilot study in a parking garage. Case Studies in Construction Materials, 2015, 2: 11–17

    Article  Google Scholar 

  14. Jonkers H M, Thijssen A, Muyzer G, Copuroglu O, Schlangen E. Application of bacteria as self-healing agent for the development of sustainable concrete. Ecological Engineering, 2010, 36(2): 230–235

    Article  Google Scholar 

  15. Bang S S, Galinat J K, Ramakrishnan V. Calcite precipitation induced by polyurethane-immobilized Bacillus pasteurii. Enzyme and Microbial Technology, 2001, 28(4–5): 404–409

    Article  Google Scholar 

  16. Vijay K, Murmu M, Deo S V. Bacteria based self healing concrete—A review. Construction & Building Materials, 2017, 152: 1008–1014

    Article  Google Scholar 

  17. BIS. 8112, Indian Standard 43 Grade Ordinary Portland Cement—- Specification, Bureau of Indian Standards. 1989

    Google Scholar 

  18. BIS. 383, Specifications for Coarse and Fine Aggregates from Natural Sources for Concrete. 1970

    Google Scholar 

  19. Khaliq W, Ehsan M B. Crack healing in concrete using various bio influenced self-healing techniques. Construction & Building Materials, 2016, 102: 349–357

    Article  Google Scholar 

  20. BIS. 10262, Guidelines for Concrete Mix Design Proportioning, Bureau of Indian Standards. 2009

    Google Scholar 

  21. Alsanusi S, Bentaher L. Prediction of compressive strength of concrete from early age test result using design experiments (RSM). World Academy of Science, Engineering and Technology International Journal of Civil and Environmental Engineering, 2011, 9(12): 978–984

    Google Scholar 

  22. Vu-Bac N, Silani M, Lahmer T, Zhuang X, Rabczuk T. A unified framework for stochastic predictions of mechanical properties of polymeric nanocomposites. Computational Materials Science, 2015, 96(Part B): 520–535

    Article  Google Scholar 

  23. Vu-Bac N, Rafiee R, Zhuang X, Lahmer T, Rabczuk T. Uncertainty quantification formultiscale modeling of polymer nanocomposites with correlated parameters. Composites. Part B, Engineering, 2015, 68: 446–464

    Article  Google Scholar 

  24. Vu-Bac N, Lahmer T, Zhuang X, Nguyen-Thoi T, Rabczuk T. A software framework forprobabilistic sensitivity analysis for computationally expensive models. Advances in Engineering Software, 2016, 100: 19–31

    Article  Google Scholar 

  25. Vu-Bac N, Lahmer T, Keitel H, Zhao J, Zhuang X, Rabczuk T. Stochastic predictions of bulk properties of amorphous polyethylene based on molecular dynamics simulations. Mechanics of Materials, 2014, 68, 70–84

    Article  Google Scholar 

  26. Vu-Bac N, Lahmer T, Zhang Y, Zhuang X, Rabczuk T. Stochastic predictions of interfacial characteristic of polymeric nanocomposites (PNCs). Composites. Part B, Engineering, 2014, 59: 80–95

    Article  Google Scholar 

  27. Badawy M F, Msekh M A, Hamdia K M, Steiner M K, Lahmer T, Rabczuk T. Hybrid nonlinear surrogate models for fracture behavior of polymeric nanocomposites. Probabilistic Engineering Mechanics, 2017, 50: 64–75

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Kunamineni Vijay.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Vijay, K., Murmu, M. Effect of calcium lactate on compressive strength and self-healing of cracks in microbial concrete. Front. Struct. Civ. Eng. 13, 515–525 (2019). https://doi.org/10.1007/s11709-018-0494-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11709-018-0494-2

Keywords

Navigation