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A cytoprotective graphene oxide-polyelectrolytes nanoshell for single-cell encapsulation

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Abstract

Graphene oxide (GO) has been increasingly utilized in the fields of food, biomedicine, environment and other fields because of its benign biocompatible. We encapsulated two kinds of GO with different sizes on yeast cells with the assistance of polyelectrolytes poly (styrene sulfonic acid) sodium salt (PSS) and polyglutamic acid (PGA) (termed as Y@GO). The result does not show a significant difference between the properties of the two types of Y@GO (namely Y@GO1 and Y@GO2). The encapsulation layers are optimized as Yeast/PGA/PSS/PGA/GO/PGA/PSS based on the morphology, dispersity, colony-forming unit, and zeta potential. The encapsulation of GO increases the roughness of the yeast. It is proved that the Y@GO increases the survival time and enhance the activity of yeast cells. The GO shell improves the resistance of yeast cells against pH and salt stresses and extends the storage time of yeast cells.

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Acknowledgements

This work is supported by the National Key Research and Development Project of China (Grant No. 218YFA0903000), the National Natural Science Foundation of China (Grant Nos. 21606013, 21301015), National Mega-project for Innovative Drugs (Grant No. 2019ZX09721001-007-002), and Shenzhen Science and Technology Project (Grant No. JCYJ20180507183842516).

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  1. These authors contributed equally to this work.

Authors and Affiliations

  1. College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, China

    Luanying He, Yulin Chang, Junhao Zhu, Ying Bi, Wenlin An, Yiyang Dong, Jia-Hui Liu & Shihui Wang

  2. Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China

    Jia-Hui Liu

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  1. Luanying He
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  2. Yulin Chang
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  3. Junhao Zhu
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  4. Ying Bi
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  5. Wenlin An
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  6. Yiyang Dong
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  7. Jia-Hui Liu
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  8. Shihui Wang
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Correspondence to Jia-Hui Liu or Shihui Wang.

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He, L., Chang, Y., Zhu, J. et al. A cytoprotective graphene oxide-polyelectrolytes nanoshell for single-cell encapsulation. Front. Chem. Sci. Eng. 15, 410–420 (2021). https://doi.org/10.1007/s11705-020-1950-9

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  • DOI: https://doi.org/10.1007/s11705-020-1950-9

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