Abstract
In this study, polyphenols from sweet potato leaves were purified by macroporous resin, and antioxidant activity was evaluated by free radical scavenging. AB-8, D-101, HPD-100 and DM301 four resins were selected for purification. The antioxidant activity was evaluated by 2,4-dinitrophenylhydrazine (DPPH) and 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulfonate) (ABTS) methods. The results showed that the DM-301 macroporous adsorption resin was most suitable by static adsorption–desorption method, and the optimal purification conditions were determined by single factor experiment as follows: the concentration of sample solution 6.0 mg/mL, the flow rate of sample solution 60 mL/min, the volume fraction of ethanol desorption 60%, and the flow rate 40 mL/h. The scavenging rates of DPPH and ABTS free radicals by purified polyphenols from sweet potato leaves were 74.04% and 98.20%, respectively. The research results provide reference for further development and utilization of polyphenols from sweet potato leaves.
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Funding
Fund projects: Shandong Vegetable Industry Technology System (SDAIT-05); The scientific research project of young teachers of Shandong Agriculture and Engineering University, "Extraction, component analysis and antioxidant activity of polyphenols in foods" (project number: QNKZY201902); "Postharvest quality control and comprehensive utilization of characteristic agricultural products", the key laboratory of Shandong Provincial Department of Education.
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Yang, LF., Nie, W., Cui, YP. et al. Purification with macroporous resin and antioxidant activity of polyphenols from sweet potato leaves. Chem. Pap. 78, 181–188 (2024). https://doi.org/10.1007/s11696-023-03058-x
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DOI: https://doi.org/10.1007/s11696-023-03058-x