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Rapid and sensitive colorimetric detection of ascorbic acid using carboxymethyl cellulose functionalized Co3O4 nanoparticles as peroxidase mimic

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Abstract

Herein we demonstrate the synthesis, characterization, and peroxidase-mimicking behavior of carboxymethyl cellulose-Co3O4 nanoparticles (C@NZ3) toward rapid and sensitive colorimetric detection of ascorbic acid (AA). AA was detected by monitoring the quenching of color signal that was obtained from C@NZ3 catalyzed H2O2 oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB). The excellent peroxidase-mimicking behavior of C@NZ3 is evidenced by the low detection limit of AA (0.41 μM) and good linearity in the range of 0.5–30 μM. The method is cost-effective, highly selective, and requires only 3 min to complete. C@NZ3 exhibited high shelf life and could be successfully used to detect AA in real samples of human serum.

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Acknowledgements

Authors thank MNIT, Jaipur, for FTIR, SEM, TEM facilities, and department of physics, University of Allahabad, Prayagraj, for XRD instrumental facility. Author Shehala thanks UGC, New Delhi, for the financial support in the form of Junior Research Fellowship (Award No.-19/06/2016 (I) EU-V).

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  1. Department of Chemistry, University of Allahabad, Prayagraj, 211002, India

    Shehala, Puneet Tiwari, Tulika Malviya & Vandana Singh

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  1. Shehala
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Correspondence to Vandana Singh.

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Shehala, Tiwari, P., Malviya, T. et al. Rapid and sensitive colorimetric detection of ascorbic acid using carboxymethyl cellulose functionalized Co3O4 nanoparticles as peroxidase mimic. Chem. Pap. 77, 5441–5453 (2023). https://doi.org/10.1007/s11696-023-02876-3

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