Abstract
In this work, an analytical procedure to determine moxidectin (MOX) and abamectin (ABA) in bovine plasma by spectrofluorometry and HPLC-FLD was developed and validated. Because their lack of native fluorescence, MOX and ABA were derivatized with trifluoroacetic anhydride (TFAA) and N-methylimidazole (NMI).Query. The excitation and emission wavelengths were 390 and 450 nm, respectively. MOX and ABA were extracted from bovine plasma with isooctane without a further clean-up. The linear range was 0–0.025 μg mL−1 for MOX and 0–0.05 μg mL−1 for ABA, with R2 values ≥ 0.98. Recoveries of MOX and ABA from spiked bovine plasma were in the range 80–102% (RSD < 14%) with spectrofluorometry and 88–102% (RSD < 10%) with HPLC-FLD. The total analysis time of the developed procedures was comparable or lower than published analytical procedures. The spectrofluorimetric method is a simpler, easier, and cheaper alternative to chromatographic procedures (HPLC) for the determination of MOX and ABA in bovine plasma samples.
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Acknowledgements
The research received financial support by a Consejo Nacional de Ciencia y Tecnología-Ciencia Básica (253578) grant. Patricia Esmeralda Vázquez-Quintal received a scholarship from CONACyT to study for a PhD in Livestock Sciences at the FMVZ-UADY. We thank to Drs. Jesus Alberto Barron Zambrano, Eduardo Gutiérrez Blanco and Alberto Rosado Aguilar for their useful suggestions for this manuscript. Also, we express our gratitude to Mariela Lope Navarrete and Dra. Melina Ojeda for their technical support to this work.
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Vázquez-Quintal, P.E., Rodríguez-Vivas, R.I. & Muñoz-Rodríguez, D. Liquid–liquid extraction for the spectrofluorimetric determination of moxidectin or abamectin in bovine plasma. Chem. Pap. 76, 7441–7449 (2022). https://doi.org/10.1007/s11696-022-02383-x
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DOI: https://doi.org/10.1007/s11696-022-02383-x