Abstract
Hibiscus sabdariffa Linn. (Roselle) is a good source of the anti-diabetic agents like cyanidin-3-sambubioside, delphinidin-3-sambubioside, and delphinidin-3-glucoside. However, their rapid degradation nullifies their maximal dosage delivery to the human body to adequately impart their therapeutic benefits. Hence, this work aimed to improve the absorption of anti-diabetic components in the Hibiscus sabdariffa Linn (roselle) extract through oral consumption of a w/o nanoemulsion prepared using an integrated low-energy phase inversion and high-energy ultrahomogenization method. The water-in-oil nanoemulsions (w/o) were screened to identify the optimal condition for preparing stable nanoemulsions with the lowest mean droplet size (MDS) of < 150 nm. The best nanoemulsion composition yielded a MDS and polydispersity index of 133.6 nm and 0.165, respectively. The low conductivity (5 µScm−1) alongside the absence of phase separation under an accelerated stability study (centrifugal force and freeze–thaw cycles) and storage (4, 25, and 40 °C) for up to 71 days verified the w/o nanoemulsion’s identity and its shelf-life stability. The abovementioned data collectively verified the stability of the roselle extract w/o nanoemulsion. The first two-hour in vitro release study showed the liberation of 85% and 60% of the bioactive ingredients at pH 6.8 and pH 2.5, respectively. The latter’s nonlinear release kinetics indicated a longer residence time that supported a more gradual release of the active ingredients. This is the first report detailing the preparation of an orally consumed roselle extract w/o nanoemulsion as an anti-diabetic agent. The formulated w/o nanoemulsion was suitable for the gradual release of the anti-diabetic agents through the simulated gastrointestinal tract.
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References
Abdel-Moemin AR (2016) Effect of roselle calyces extract on the chemical and sensory properties of functional cupcakes. Food Sci Hum Wellness 5(4):230–237. https://doi.org/10.1016/j.fshw.2016.07.003
Abdou ES, Galhoum GF, Mohamed EN (2018) Curcumin loaded nanoemulsions/pectin coatings for refrigerated chicken fillets. In: Food hydrocolloids, vol 83. Elsevier Ltd. https://doi.org/10.1016/j.foodhyd.2018.05.026
Alkhamaiseh SI, Aljofan M (2020) Prevalence of use and reported side effects of herbal medicine among adults in Saudi Arabia. Complement Ther Med. https://doi.org/10.1016/j.ctim.2019.102255
Amor BB, Allaf K (2009) Impact of texturing using instant pressure drop treatment prior to solvent extraction of anthocyanins from Malaysian Roselle (Hibiscus sabdariffa). Food Chem 115(3):820–825. https://doi.org/10.1016/j.foodchem.2008.12.094
Aswathanarayan JB, Vittal RR (2019). Nanoemulsions and their potential applications in food industry. 3:1–21.https://doi.org/10.3389/fsufs.2019.00095
Azhar SNAS, Ashari SE, Salim N (2018) Development of a kojic monooleate-enriched oil-in-water nanoemulsion as a potential carrier for hyperpigmentation treatment. Int J Nanomed 13:6465. https://doi.org/10.2147/IJN.S171532
Chambial S, Dwivedi S, Shukla KK, John PJ, Sharma P (2013) Vitamin C in disease prevention and cure: an overview. Indian J Clin Biochem IJCB 28(4):314–328. https://doi.org/10.1007/s12291-013-0375-3
Cichero JAY (2013) Thickening agents used for dysphagia management: effect on bioavailability of water, medication and feelings of satiety. Nutr J 12(1):1–8. https://doi.org/10.1186/1475-2891-12-54
Cid-Ortega S, Guerrero-Beltrán JA (2015) Roselle calyces (Hibiscus sabdariffa), an alternative to the food and beverages industries: a review. J Food Sci Technol 52:6859–6869. https://doi.org/10.1007/s13197-015-1800-9
Cunningham E (2009) What impact does pH have on food and nutrition? J Am Diet Assoc 109(10):1816. https://doi.org/10.1016/j.jada.2009.08.028
Dlugaszewska J, Ratajczak M, Kamińska D, Gajecka M (2019) Are dietary supplements containing plant-derived ingredients safe microbiologically? Saudi Pharm J 27(2):240–245. https://doi.org/10.1016/j.jsps.2018.11.005
Goindi S, Kaur A, Kaur R, Kalra A, Chauhan P (2016) 19—Nanoemulsions: an emerging technology in the food industry. In: Emulsions. Elsevier Inc. https://doi.org/10.1016/B978-0-12-804306-6/00019-2
Gouda R, Baishya H, Qing Z (2017) Application of mathematical models in drug release kinetics of carbidopa and levodopa ER tablets. J Dev Drugs 6(02):1–8. https://doi.org/10.4172/2329-6631.1000171
Harwansh RK, Deshmukh R, Rahman MA (2019) Nanoemulsion: promising nanocarrier system for delivery of herbal bioactives. J Drug Deliv Sci Technol 51:224–233. https://doi.org/10.1016/j.jddst.2019.03.006
Haziqah N, Marzuki C, Wahab RA, Hamid MA (2019) An overview of nanoemulsion : concepts of development and cosmeceutical applications. Biotechnol Biotechnol Equip 33(1):779–797. https://doi.org/10.1080/13102818.2019.1620124
Hidajat MJ, Jo W, Kim H, Noh J (2020) Effective droplet size reduction and excellent stability of limonene nanoemulsion formed by high-pressure homogenizer. Colloids Interfaces 4(1):5. https://doi.org/10.3390/colloids4010005
Jabeur I, Pereira E, Barros L, Calhelha RC, Soković M, Oliveira MBP, Ferreira IC (2017) Hibiscus sabdariffa L. as a source of nutrients, bioactive compounds and colouring agents. Food Res Int 100:717–723. https://doi.org/10.1016/j.foodres.2017.07.073
Kamel FO, Magadmi RM, Hagras MM, Magadmi B, AlAhmad RA (2017) Knowledge, attitude, and beliefs toward traditional herbal medicine use among diabetics in Jeddah Saudi Arabia. Complement Ther Clin Pract 29:207–212. https://doi.org/10.1016/j.ctcp.2017.10.007
Kheynoor N, Hosseini SMH, Yousefi GH, Hashemi Gahruie H, Mesbahi GR (2018) Encapsulation of vitamin C in a rebaudioside-sweetened model beverage using water in oil in water double emulsions. LWT 96:419–425. https://doi.org/10.1016/j.lwt.2018.05.066
Kim HS, Mason TG (2017) Advances and challenges in the rheology of concentrated emulsions and nanoemulsions. Adv Coll Interface Sci 247:397–412. https://doi.org/10.1016/j.cis.2017.07.002
Komaiko JS, Mcclements DJ (2016) Formation of food-grade nanoemulsions using low-energy preparation methods: a review of available methods. Compr Rev Food Sci Food Saf 15(2):331–352. https://doi.org/10.1111/1541-4337.12189
Koziolek M, Grimm M, Becker D, Iordanov V, Zou H, Shimizu J, Wanke C, Garbacz G, Weitschies W (2015) Investigation of pH and temperature profiles in the GI tract of fasted human subjects using the Intellicap® system. J Pharm Sci 104(9):2855–2863. https://doi.org/10.1002/jps.24274
Liang R, Shoemaker CF, Yang X, Zhong F, Huang Q (2013) Stability and bioaccessibility of β-carotene in nanoemulsions stabilized by modified starches. J Agric Food Chem 61(6):1249–1257. https://doi.org/10.1021/jf303967f
Liu Q, Huang H, Chen H, Lin J, Wang Q (2019) Food-grade nanoemulsions: preparation, stability and application in encapsulation of bioactive compounds. Molecules 24(23):4242. https://doi.org/10.3390/molecules24234242
Marwaha RK, Dabas A (2019) Bioavailability of nanoemulsion formulations vs conventional fat soluble preparations of cholecalciferol (D3)—an overview. J Clin Orthop Trauma 10(6):1094–1096. https://doi.org/10.1016/j.jcot.2019.07.014
Mat Hadzir N, Basri M, Abdul Rahman MB, Salleh AB, Raja Abdul Rahman RNZ, Basri H (2013) Phase behaviour and formation of fatty acid esters nanoemulsions containing piroxicam. AAPS PharmSciTech 14(1):456–463. https://doi.org/10.1208/s12249-013-9929-1
McClements DJ, Jafari SM (2018) General aspects of nanoemulsions and their formulation. In: Nanoemulsions. Academic Press, pp 3–20. https://doi.org/10.1016/B978-0-12-811838-2.00001-1
Musa SH, Basri M, Masoumi HRF, Shamsudin N, Salim N (2017) Enhancement of physicochemical properties of nanocolloidal carrier loaded with cyclosporine for topical treatment of psoriasis: in vitro diffusion and in vivo hydrating action. Int J Nanomed 12:2427. https://doi.org/10.2147/IJN.S125302
Nagi A, Iqbal B, Kumar S, Sharma S, Ali J, Baboota S (2017) Quality by design based silymarin nanoemulsion for enhancement of oral bioavailability. J Drug Deliv Sci Technol 40:35–44. https://doi.org/10.1016/j.jddst.2017.05.019
Neen S, Utra U, Karim A, Boon T, Ping C, Shari N (2020) Physical, morphological and antibacterial properties of lime essential oil nanoemulsions prepared via spontaneous emulsi fi cation method. LWT 128:109388. https://doi.org/10.1016/j.lwt.2020.109388
Nejadmansouri M, Hosseini SMH, Niakosari M, Yousefi GH, Golmakani MT (2016) Physicochemical properties and oxidative stability of fish oil nanoemulsions as affected by hydrophilic lipophilic balance, surfactant to oil ratio and storage temperature. Colloids Surf A Physicochem Eng Asp 506:821–832. https://doi.org/10.1016/j.colsurfa.2016.07.075
Obafemi TO, Olaleye MT, Akinmoladun AC (2019) Anti-diabetic property of miracle fruit plant (Synsepalum dulcificum Shumach. & Thonn. Daniell) leaf extracts in fructose-fed streptozotocin-injected rats via anti-inflammatory activity and inhibition of carbohydrate metabolizing enzymes. J Ethnopharmacol 244:112124. https://doi.org/10.1016/j.jep.2019.112124
Okuro PK, Gomes A, Costa ALR, Adame MA, Cunha RL (2019) Formation and stability of W/O-high internal phase emulsions (HIPEs) and derived O/W emulsions stabilized by PGPR and lecithin. Food Res Int 122:252–262. https://doi.org/10.1016/j.foodres.2019.04.028
Pei S, Ming O, Abas F, Kwong H, Ping C (2014) Stability of a concentrated oil-in-water emulsion model prepared using palm olein-based diacylglycerol/virgin coconut oil blends: effects of the rheological properties, droplet size distribution and microstructure. Food Res Int 64:919–930. https://doi.org/10.1016/j.foodres.2014.08.045
Pereira TA, Guerreiro CM, Maruno M, Ferrari M, Rocha-Filho PA (2016) Exotic vegetable oils for cosmetic O/W nanoemulsions: In vivo evaluation. Molecules 21(3):1–16. https://doi.org/10.3390/molecules21030248
Pongsumpun P, Iwamoto S, Siripatrawan U (2020) Response surface methodology for optimization of cinnamon essential oil nanoemulsion with improved stability and antifungal activity. Ultrason Sonochemistry 60:104604. https://doi.org/10.1016/j.ultsonch.2019.05.021
Rai VK, Mishra N, Yadav KS, Yadav NP (2018) Nanoemulsion as pharmaceutical carrier for dermal and transdermal drug delivery: formulation development, stability issues, basic considerations and applications. J Control Release 270:203–225. https://doi.org/10.1016/j.jconrel.2017.11.049
Rein MJ, Renouf M, Cruz-hernandez C, Actis-goretta L, Thakkar SK, Pinto S (2012) Bioavailability of bioactive food compounds: a challenging journey to bioefficacy. Br J Clin Pharmacol 75(3):588–602. https://doi.org/10.1111/j.1365-2125.2012.04425.x
Riaz G, Chopra R (2018) A review on phytochemistry and therapeutic uses of Hibiscus sabdariffa L. Biomed Pharmacother 102:575–586. https://doi.org/10.1016/j.biopha.2018.03.023
Roselan MA, Ashari SE, Faujan NH, Mohd Faudzi SM, Mohamad R (2020) An improved nanoemulsion formulation containing Kojic Monooleate: optimization, characterization and in vitro studies. Molecules 25(11):2616. https://doi.org/10.3390/molecules25112616
Saberi AH, Fang Y, McClements DJ (2013) Fabrication of vitamin E-enriched nanoemulsions: factors affecting particle size using spontaneous emulsification. J Colloid Interface Sci 391:95–102. https://doi.org/10.1016/j.jcis.2012.08.069
Saeedi P, Petersohn I, Salpea P, Malanda B, Karuranga S, Unwin N, Colagiuri S, Guariguata L, Motala AA, Ogurtsova K, Shaw JE, Bright D, Williams R (2019) Global and regional diabetes prevalence estimates for 2019 and projections for 2030 and 2045: Results from the International Diabetes Federation Diabetes Atlas. Diabetes Res Clin Pract 157:107843. https://doi.org/10.1016/j.diabres.2019.107843
Samson S, Basri M, Fard Masoumi HR, Abdul Malek E, Abedi Karjiban R (2016a) An artificial neural network based analysis of factors controlling particle size in a virgin coconut oil-based nanoemulsion system containing copper peptide. PLoS ONE 11(7):1–15. https://doi.org/10.1371/journal.pone.0157737
Samson S, Basri M, Reza H, Masoumi F, Abedi R, Malek EA (2016b) Design and development of a nanoemulsion system containing copper peptide by D-optimal mixture design and evaluation of its physicochemical properties. RSC Adv 6:17845–17856. https://doi.org/10.1039/C5RA24379C
Shahidan NS, Salim N, Ashari SE (2019) Preparation and optimization of ibuprofen-loaded nanoemulsion formulation. J Multidiscip Eng Sci Technol 6:89–96
Sulaiman ISC, Basri M, Masoumi HRF, Ashari SE, Ismail M (2016) Design and development of a nanoemulsion system containing extract of Clinacanthus nutans (L.) leaves for transdermal delivery system by D-optimal mixture design and evaluation of its physicochemical properties. RSC Adv 6(71):67378–67388. https://doi.org/10.1039/C6RA12930G
Szymczyk K, Zdziennicka A, Jańczuk B (2018) Adsorption and aggregation properties of some polysorbates at different temperatures. J Solut Chem 47(11):1824–1840. https://doi.org/10.1007/s10953-018-0823-z
Ting Y, Jiang Y, Ho CT, Huang Q (2014) Common delivery systems for enhancing in vivo bioavailability and biological efficacy of nutraceuticals. J Funct Foods 7:112–128. https://doi.org/10.1016/j.jff.2013.12.010
Wang S, Wang X, Liu M, Zhang L (2020) Preparation and characterization of Eucommia ulmoides seed oil O/W nanoemulsion by dynamic high-pressure microfluidization. LWT 121:108960. https://doi.org/10.1016/j.lwt.2019.108960
Xiong K, Zhou L, Wang J, Ma A, Fang D, Xiong L, Sun Q (2020) Construction of food-grade pH-sensitive nanoparticles for delivering functional food ingredients. Trends Food Sci Technol 96:102–113. https://doi.org/10.1016/j.tifs.2019.12.019
Zannou O, Kelebek H, Selli S (2020) Elucidation of key odorants in Beninese Roselle (Hibiscus sabdariffa L.) infusions prepared by hot and cold brewing. Food Res Int 133:109133. https://doi.org/10.1016/j.foodres.2020.109133
Zhang L, Han C, Liu M, Yang H, Zhang F, Liu B, Meng X (2020) The formation, stability of DHA/EPA nanoemulsion prepared by emulsion phase inversion method and its application in apple juice. Food Res Int 133:109132. https://doi.org/10.1016/j.foodres.2020.109132
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This research was supported by Ministry of Education (MOE) through the Fundamental Research Grant Scheme (FRGS/1/ 2020/ST G01/UTM/02/8).
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Abdul Wahab, R., Al-obaidi, N.G., Yahya, N.A. et al. Formulation of a stable water-in-oil nanoemulsion rich in anti-diabetic components of the roselle extract for controlled release. Chem. Pap. 76, 2341–2356 (2022). https://doi.org/10.1007/s11696-021-02030-x
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DOI: https://doi.org/10.1007/s11696-021-02030-x