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Effect of Sleeve Gastrectomy on Angiogenesis and Adipose Tissue Health in an Obese Animal Model of Type 2 Diabetes

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Abstract

Introduction

Metabolic surgery has become an accepted option for the treatment of obesity and associated metabolic diseases like hypertension and type 2 diabetes. Adipose tissue dysfunction and ectopic storage of excess lipids are thought to be involved in the underlying pathophysiological process.

Objectives

The present study aims to clarify the effect of sleeve gastrectomy (SG) on adipose tissue microvasculature and health in an animal model of adipose type 2 diabetes.

Methods

After weaning, diabetic Goto-Kakizaki rats were either fed on standard rat chow or high-calorie diet. At 4 months, animals on high-calorie diet were randomized to SG, sham surgery, or control group. Non-diabetic Wistar rats served as further controls. At 6 months, glucose and lipid metabolisms were studied in vivo. After sacrifice, periepididymal adipose tissue was collected for histology and analysis of parameters of adipose tissue metabolism and insulin sensitivity.

Results

SG decreased body and adipose tissue weight and improved glycemic and lipid profiles. Fasting glycemia, area under the curve after intraperitoneal insulin tolerance test, and insulin resistance were decreased in operated animals. SG also reduced circulating triglycerides and cholesterol while increasing serum adiponectin and adipose tissue peroxisome proliferator-activated receptor γ (PPAR-γ) and perilipin A. Additionally, surgery improved adipose tissue vascular function and markedly increased vascular endothelial growth factor, cluster of differentiation 31, and endothelial nitric oxide synthase.

Conclusions

In our obese animal model of type 2 diabetes, SG significantly improved adipose tissue health and angiogenesis while reducing insulin resistance, involving PPAR-γ and markers of sprouting angiogenesis and endothelial function.

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Funding

This study was supported by the Portuguese Foundation for Science and Technology (Consortium CNC.IBILI UID/NEU/04539/2013; SFRH/BD/101172/2014 and SFRH/BPD/104881/2014) and by the Faculty of Medicine, University of Coimbra.

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Authors and Affiliations

  1. Obesity Center, Hospital da Luz Setúbal, EN 10, km 37, 2900-722, Setubal, Portugal

    Hans Eickhoff

  2. Institute of Physiology and Institute for Clinical and Biomedical Research (iCBR), Faculty of Medicine, University of Coimbra, Polo III, Azinhaga de Santa Comba, Celas, 3000-548, Coimbra, Portugal

    Hans Eickhoff, Tiago Rodrigues, Inês Neves, Daniela Marques, Diana Ribeiro, Susana Costa, Raquel Seiça & Paulo Matafome

  3. Coimbra Health School, Polytechnic Institute of Coimbra, Avenida Dr Marnoco e Sousa 30, 3000-271, Coimbra, Portugal

    Paulo Matafome

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  1. Hans Eickhoff
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Correspondence to Hans Eickhoff.

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The study protocol regarding the use of laboratory animals was approved by the Institutional Ethics Committee. After completing the study, all animals were sacrificed by cervical displacement, according to the procedures approved by the Institutional Animal Care and Use Committee.

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Eickhoff, H., Rodrigues, T., Neves, I. et al. Effect of Sleeve Gastrectomy on Angiogenesis and Adipose Tissue Health in an Obese Animal Model of Type 2 Diabetes. OBES SURG 29, 2942–2951 (2019). https://doi.org/10.1007/s11695-019-03935-z

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