Abstract
As a step toward applications for biosensors, we characterized the electrical properties of λDNA molecules via their current–voltage characteristics and complex impedance plots. λDNA molecules were introduced to a microfluidic device using a microchannel (depth, 50 μm; width, 500 μm; length, 10 mm) and electrostatically stretched and immobilized in the 14-μm gap between two triangular-shaped microlithographed aluminum electrodes by applying an alternating voltage of 1 MHz and 20 Vp–p. The aligned λDNA showed nonlinear current–voltage characteristics. From the complex impedance plots of the λDNA molecules, an equivalent circuit was obtained as a series connection of two resistance–capacitance parallel circuits. Finally, we demonstrated that the electrical characteristics of the λDNA between the electrodes varied with the number of immobilized λDNA molecules.
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Acknowledgments
This work was partially supported by a Grant-in-Aid for Young Scientists (Grant No.: JP18K13769) and a Grant-in-Aid for Challenging Exploratory Research (Grant No.: JP16K14281) from JSPS. The authors gratefully thank Dr. Masanori Eguchi of the National Institute of Technology, Kure College for his help with electron-beam lithography exposure and I–V measurements. We are grateful to Prof. Seiichi Suzuki of Seikei University for his help with fluorescence observation.
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Himuro, T., Tsukamoto, S. & Saito, Y. Electrical Evaluation of DNA Stretched and Immobilized Between Triangular-Shaped Electrodes. J. Electron. Mater. 48, 1562–1567 (2019). https://doi.org/10.1007/s11664-018-06899-5
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DOI: https://doi.org/10.1007/s11664-018-06899-5