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Protective effects of Xiongshao Capsule (芎芍胶囊) on anti-inflammatory function of high-density lipoprotein in an atherosclerosis rabbit model

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Abstract

Objective

To observe the effects of Xiongshao Capsule (芎芍胶囊, XSC) on anti-inflflammatory properties of high-density lipoprotein (HDL), myeloperoxidase (MPO) and paraoxonase 1 (PON1) in serum of atherosclerosis (AS) rabbit model and explore the anti-inflflammatory protective effects of XSC on HDL.

Methods

Sixty rabbits were randomized into the control, the model, XSC low-, medium- and high-dose (Rhizoma Chuanxiong + Radix Paeoniae rubra: 0.6+0.3, 1.2+0.6, 2.4+1.2g·kg-1·day-1, respectively), and simvastatin (1g·kg-1·day-1) groups. The model rabbits were fed with high-fat diet and drugs for 15 weeks. The blood and thoracic aortas samples were collected at the end of 15 weeks. The levels of serum MPO and PON1 as well as total cholesterol (TC) and free cholesterol (FC) in aorta wall cells were tested by enzyme linked immunosorbent assay.

Results

TC and FC in the model group were significantly higher than those in the control group (P<0.01). Compared with the model group, TC and FC in the XSC groups were signifificantly lower (P<0.05 or P<0.01), so was simvastatin group (P<0.01). There was no signifificant difference in PON1 level between groups (P>0.05), even between model and control groups (P>0.05). The serum MPO level in the model group was signifificantly higher than that in the control group (P<0.05), which was signifificantly lower in XSC groups as well as simvastatin group (P<0.05 or P<0.01), and no difference was found between XSC groups and simvastatin group (P>0.05).

Conclusions

XSC can reduce the serum MPO level in AS rabbits to protect the anti-inflammatory function of HDL, maintaining the normal lipid transport function. TC and FC levels in aorta cells decline, and this process initiated by XSC plays an anti-AS role.

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References

  1. Verdier C, Martinez LO, Ferrières J, Elbaz M, Genoux A, Perret B. Targeting high-density lipoproteins: update on a promising therapy. Arch Cardiovasc Dis 2013;106:601–611.

    Article  PubMed  Google Scholar 

  2. Sorci-Thomas MG, Thomas MJ. Why targeting HDL should work as a therapeutic tool, but has not. J Cardiovasc Pharmacol 2013;62:239–246.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Alwaili K, Awan Z, Alshahrani A, Genest J. High-density lipoproteins and cardio-vascular disease: 2010 update. Expert Rev Cardiovasc Ther 2010;8:413–423.

    Article  CAS  PubMed  Google Scholar 

  4. De la Llera Moya M, McGillicuddy FC, Hinkle CC, Byrne M, Joshi MR, Nguyen V, et al. Inflammation modulates human HDL composition and function in vivo. Atherosclerosis 2012;222:390–394.

    Article  PubMed  PubMed Central  Google Scholar 

  5. Ansell BJ, Watson KE, Fogelman AM, Navab M, Fonarow GC. High-density lipoprotein function recent advances. J Am Coll Cardiol 2005;46:1792–1798.

    Article  CAS  PubMed  Google Scholar 

  6. Kotosai M, Shimada S, Kanda M, Matsuda N, Sekido K, Shimizu Y, et al. Plasma HDL reduces non-esterified fatty acid hydroperoxides originating from oxidized LDL: a mechanism for its antioxidant ability. Lipids 2013;48:569–578.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Li LZ, Liu JG, Ma LB, Lu XY, Xu H, Xu FQ, et al. Effect of Xiongshao Capsule on lipid metabolism and platelet aggregation in experimental atherosclerosis rabbits. Chin J Integr Tradit West Med (Chin) 2008;28:1100–1103.

    CAS  Google Scholar 

  8. Barter P. Lessons learned from the investigation of lipid level management to understand its impact in atherosclerotic events (ILLUMINATE) trial. Am J Cardiol 2009;104(10 Suppl):10E–5E.

    Article  CAS  PubMed  Google Scholar 

  9. Triolo M, Annema W, Dullaart RP, Tietge UJ. Assessing the functional properties of high density lipoproteins: an emerging concept in cardiovascular research. Biomark Med 2013;7:457–472.

    Article  CAS  PubMed  Google Scholar 

  10. Wu JX, Liang C, Wu ZG. The functions of HDL and related research progress on atherosclerosis. Shanghai Med J (Chin) 2010;33:1062–1066.

    CAS  Google Scholar 

  11. Fuhrman B, Gantman A, Aviram M. Paraoxonase 1 (PON1) deficiency in mice is associated with reduced expression of macrophage SR-BI and consequently the loss of HDL cytoprotection against apoptosis. Atherosclerosis 2010;211:61–68.

    Article  CAS  PubMed  Google Scholar 

  12. Eren E, Yilmaz N, Aydin O. Functionally defective highdensity lipoprotein and paraoxonase: a couple for endothelial dysfunction in atherosclerosis. Cholesterol 2013;2013:792090.

    Article  PubMed  PubMed Central  Google Scholar 

  13. Zhou QY. The correlation between serum paraoxonase-1 level and types of acute coronary syndrome. Chin Gener Pract Chin 2013;16:3189–3191.

    Google Scholar 

  14. Zhou C, Cao J, Shang L, Tong C, Hu H, Wang H, et al. Reduced paraoxonase 1 activity as a marker for severe coronary artery disease. Dis Markers 2013;35:97–103.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Hewing B, Parathath S, Barrett T, Chung WK, Astudillo YM, Hamada T, et al. Effects of native and myeloperoxidase modified apolipoprotein A-I on reverse cholesterol transport and atherosclerosis in mice. Arterioscler Thromb Vasc Biol 2014;34:779–789.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  16. Shao B, Tang C, Sinha A, Mayer PS, Davenport G, Brot N, et al. Humans with atherosclerosis have impaired ABCA1 cholesterol efflux and enhanced HDL oxidation by myeloperoxidase. Circ Res 2014;114:1733–1742.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. Sokolov AV, Kostevich VA, Runova OL, Gorudko IV, Vasilyev VB, Cherenkevich SN, et al. Proatherogenic modification of LDL by surface bound myeloperoxidase. Chem Phys Lipids 2014;180:72–80.

    Article  CAS  PubMed  Google Scholar 

  18. Delporte C, Van Antwerpen P, Vanhamme L, Roumeguère T, Zouaoui Boudjeltia K. Low-density lipoprotein modifyed by myeloperoxidase in inflammatory pathways and clinical studies. Mediators Inflamm 2013;2013:971579.

    Article  PubMed  PubMed Central  Google Scholar 

  19. Rudolph TK, Schaper N, Klinke A, Demir C, Goldmann B, Lau D, et al. Liberation of vessel adherent myeloperoxidase reflects plaque burden in patients with stable coronary artery disease. Atherosclerosis 2013;231:354–358.

    Article  CAS  PubMed  Google Scholar 

  20. Huang Y, Wu Z, Riwanto M, Gao S, Levison BS, Gu X, et al. Myeloperoxidase, paraoxonase-1, and HDL form a functional ternary complex. J Clin Invest 2013;123:3815–3828.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Authors and Affiliations

  1. Department of Emergency, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, 100091, China

    Yan-hong Zhang  (张艳虹)

  2. Department of General Medicine, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, 100091, China

    Ying Zhang  (张 颖), Wen-xin Tong  (童文新) & Feng-qin Xu  (徐凤芹)

  3. Administration Office, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, 100091, China

    Jing Li  (李 婧)

Authors
  1. Yan-hong Zhang  (张艳虹)
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  2. Ying Zhang  (张 颖)
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  3. Jing Li  (李 婧)
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  4. Wen-xin Tong  (童文新)
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  5. Feng-qin Xu  (徐凤芹)
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Corresponding author

Correspondence to Feng-qin Xu  (徐凤芹).

Additional information

Supported by the National Natural Science Foundation of China (No. 81173385)

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Zhang, Yh., Zhang, Y., Li, J. et al. Protective effects of Xiongshao Capsule (芎芍胶囊) on anti-inflammatory function of high-density lipoprotein in an atherosclerosis rabbit model. Chin. J. Integr. Med. 23, 357–361 (2017). https://doi.org/10.1007/s11655-015-2298-8

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  • DOI: https://doi.org/10.1007/s11655-015-2298-8

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