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Effect of alternate-day fasting therapy combined with Linggui Zhugan Decoction (苓桂术甘汤) on hepatic oxidative stress in hyperlipidemic rat

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Abstract

Objective

To observe the effect of alternate-day fasting (ADF) therapy combined with Linggui Zhugan Decoction (苓桂术甘汤, LZD) on hepatic oxidative stress and blood lipids in hyperlipidemic rats.

Methods

Fifty-two Wistar rats were randomly assigned to two groups: the high-fat-diet (HF) group and the normal-diet (ND) group. Hyperlipidemia was induced by feeding rats with high-cholesterol-diet for 5 weeks. Then the HF group was randomized to the model-control (MC) group, the alternate-day-fasting (ADF) group, and the ADF combined with LZD (AL) group. The ND group was regarded as the negative control (NC) group. The AL and ADF groups were put on fast for 24 h on alternate days for 4 weeks. The AL group was administrated with LZD on the fast days. Body weight and food intake were measured once a week. After 4-week ADF, blood sample was collected for determination of plasma total cholesterol (TC), triglyceride (TG), low-density-lipoprotein cholesterol (LDL-C) and high-density-lipoprotein cholesterol (HDL-C). Liver oxidative stress parameters including total superoxide dismutase (T-SOD) activity, malondialdehyde (MDA) level and glutathione (GSH) content were also tested.

Results

Body weight in the HF group decreased significantly (P<0.01). TC, TG, HDL-C, and LDL-C concentrations in the HF group were higher than those in the NC group (P<0.01), respectively. T-SOD in the HF group was clearly lower than that in the NC group (P<0.05). After 4-week intervention, body weight, TC and TG concentrations in the ADF and AL groups declined significantly, respectively, compared to MC group (P<0.05). GSH in the ADF and AL groups were much higher than those in the MC group (P<0.01). MDA level was also greatly higher in the ADF group as compared with the NC group (P<0.05).

Conclusion

ADF therapy combined with LZD may be used as an effective combination approach for treatment of hyperlipidemia and oxidative stress damage.

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References

  1. Ke B, Qin J, Meng J, Wu ZZ, Rampp T, Michelsan A. Research progress of fasting therapy. Shenzhen J Integr Tradit Chin West Med 2009;19:55–57.

    Google Scholar 

  2. Qin J. Fasting therapy in China: modification and future development. Chin J Integr Med 2013;19:883–884.

    Article  PubMed  Google Scholar 

  3. Chen DS, Ke B, Huang YJ, Zhang JJ, Michelsan A, Qin J. Effects of the modified Linggui Zhugan Decoction (flavored Ling Gui Zhu Gan Tang) combined with short-term very low calorie diets on glycemic control in newly diagnosed type 2 diabetics. J Tradit Chin Med (Chin) 2011;3:185–188.

    Article  Google Scholar 

  4. Chen DS, Meng J, Ke B, Zhang JJ, Michelsan A, Qin J. Effect on fasting therapy for medicine subjects with blood pressure. Gansu J Tradit Chin Med (Chin) 2010, 26(6):11–12.

    CAS  Google Scholar 

  5. Chen DS, Li CY, Michelsan A, Kessler C, Huang YJ, Meng J, et al. Modified Ling-Gui-Zhu-Gan Decoction combined with short-term fasting improves therapeutic response in type 2 diabetic patients. Eur J Integr Med 2012:73:32–35.

    Google Scholar 

  6. Ke B, Shi L, Zhang JJ, Chen DS, Meng J, Qin J. Protective effects of modified Linggui Zhugan Decoction combined with short-term very low calorie diets on cardiovascular risk factors in obese patients with impaired glucose tolerance. J Tradit Chin Med (Chin) 2012;33:185–188.

    Google Scholar 

  7. Zhang JJ, Ke B, Qin J. Intermittent fasting research progress. Med Recapit (Chin) 2012;18:1332–1335.

    CAS  Google Scholar 

  8. Zhao W, Sun GZ. The dosage conversion between different kinds of experimental animals. Animal Husbandry Veterinary Sci Techn Inform (Chin) 2012;5:52–53.

    Google Scholar 

  9. Sorensen M, Sanz A, Gómez J, Pamplona R, Portero-Otin M, Gredilla R. et al. Effects of fasting on oxidative stress in rat liver mitochondria. Free Radic Res 2006;40:339–347.

    Article  CAS  PubMed  Google Scholar 

  10. Marczuk-Krynicka D, Hryniewiecki T, Piatek J, Paluszak J. The effect of brief food withdrawal on the level of free radicals and other parameters of oxidative status in the liver. Med Sci Monitor 2003;9:131–135.

    Google Scholar 

  11. Bhutani, S, Klempel MC, Berger RA, Varady KA. Improvements in coronary heart disease risk indicators is by alternate-day fasting involve adipose tissue modulations. Obesity 2010;11:2152–2159.

    Article  Google Scholar 

  12. Descamps O, Riondel J, Ducros V, Roussel AM. Mitochondrial production of reactive oxygen species and incidence of ageassociated lymphoma in OF1 mice: effect of alternate-day fasting. Mech Ageing Dev 2005;126:1185–1191.

    Article  CAS  PubMed  Google Scholar 

  13. Calabrese EJ. Converging concepts: adaptive response, preconditioning, and the Yerkes-Dodson Law are manifestations of hormesis. Ageing Res Rev 2008;7:8–20.

    Article  CAS  PubMed  Google Scholar 

  14. Le Bourg E. Hormesis, aging and longevity. Biochim Biophys Acta 2009;1790:1030–1039.

    Article  PubMed  Google Scholar 

  15. Zhang Q, Jiang Y, Chen JB. Experiment of effect of Ling-Gui-Zhu-Gang Decoction on blood rheology in hyperlipidemic Rat. J Chengdu Univ Tardit Chin Med (Chin) 2003;26(3):11–14.

    CAS  Google Scholar 

  16. Wang X, LI M, Wang JI. Effect of high fat diet on changes of body mass and hair in rats. Chin J Clin Rehabil (Chin) 2005;9(7):78–79.

    Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Traditional Chinese Medicine, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510080, China

    Jun-jie Zhang  (张俊杰), Ying-juan Huang  (黄颖娟), Bin Ke  (柯 斌), Li-ping Liu  (刘丽萍), Jun Meng  (孟 君) & Jian Qin  (秦 鉴)

  2. Nursing School of Sun Yat-sen University, Guangzhou, 510080, China

    Jie-jie Shangguan  (上官杰杰)

Authors
  1. Jun-jie Zhang  (张俊杰)
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  2. Ying-juan Huang  (黄颖娟)
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  3. Bin Ke  (柯 斌)
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  4. Li-ping Liu  (刘丽萍)
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  5. Jie-jie Shangguan  (上官杰杰)
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  6. Jun Meng  (孟 君)
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  7. Jian Qin  (秦 鉴)
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Correspondence to Jian Qin  (秦 鉴).

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Zhang, Jj., Huang, Yj., Ke, B. et al. Effect of alternate-day fasting therapy combined with Linggui Zhugan Decoction (苓桂术甘汤) on hepatic oxidative stress in hyperlipidemic rat. Chin. J. Integr. Med. (2015). https://doi.org/10.1007/s11655-014-1999-8

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  • DOI: https://doi.org/10.1007/s11655-014-1999-8

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