Abstract
The development of the CNS is a complex and well-regulated process, where stem cells differentiate into committed cells depending on the stimuli from the microenvironment. Alterations of oxygen levels were stated to be significant in terms of brain development and neurogenesis during embryonic development, as well as the adult neurogenesis. As a product of oxygen processing, hydrogen peroxide (H2O2) has been established as a key regulator, acting as a secondary messenger, of signal transduction and cellular biological functions. H2O2 is involved in survival, proliferation, and differentiation of neural stem cells into committed cells of the CNS. Effects of different concentrations of exogenous H2O2 on neuronal differentiation and the molecular pathways involved are yet to be clearly understood. Here, we investigated the concentration-dependent effects of H2O2 on differentiation of neural stem cells using CGR8 embryonic mouse stem cell line. We have demonstrated that treated doses of H2O2 suppress neural differentiation; additionally, our study suggests that relatively high doses of exogenous H2O2 suppress the differentiation process of neural stem cells through AKT and p38 pathways.
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This study was financially supported by Dokuz Eylul University (project number: 2018.KB.SAG.061).
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All authors contributed to the study. Material preparation, data collection, and analysis were performed by Bedir Irem Eltutan and Kemal Ugur Tufekci. Bedir Irem Eltutan and Cagla Kiser wrote the first draft of the manuscript. All authors read and confirmed the final manuscript.
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Eltutan, B.I., Kiser, C., Ercan, İ. et al. In vitro effects of H2O2 on neural stem cell differentiation. In Vitro Cell.Dev.Biol.-Animal 58, 810–816 (2022). https://doi.org/10.1007/s11626-022-00723-5
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DOI: https://doi.org/10.1007/s11626-022-00723-5