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microRNA-4429-5p suppresses the malignant development of colon cancer by targeting matrix metalloproteinase 16

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Abstract

Colon cancer has been recognized as the major reason for global cancer-associated mortality. microRNA (miRNA, miR)-4429-5p has been documented to act as a tumor-suppressive miRNA in some cancers, but its effect on colon cancer remains elusive. In this study, the biological effects of miR-4429-5p were investigated both in vitro by MTT, 5-ethynyl-2′-deoxyuridine (EdU), wound healing, and transwell assays and in vivo by a xenograft mice model. Western blot, quantitative reverse transcription-polymerase chain reaction (qRT-PCR), and dual-luciferase assay were used to identify the binding of miR-4429-5p on matrix metalloproteinase 16 (MMP16) 3′-UTR. Our results suggested that overexpression of miR-4429-5p hindered colon cancer cell proliferation, migration, and invasion, whereas knockdown of miR-4429-5p exhibited the opposite effect in colon cancer cells. Mechanistically, miR-4429-5p directly bound to the 3′-UTR of MMP16 and led to inhibition of MMP16 protein. Overexpression of miR-4429-5p inhibited colon tumor growth by targeting MMP16. Taken together, our study revealed that miR-4429-5p prevented colon cancer progression through targeting MMP16, indicating miR-4429-5p as a promising target for treatment improvement for colon cancer.

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Data availability

The data that support the findings of this study are available from the corresponding author upon reasonable request.

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Authors and Affiliations

  1. The Second Department of General Surgery, Cangzhou Central Hospital, No. 16 Xinhua West Road, Cangzhou, 061000, Hebei, China

    Wei Li, Zhe Song, Nan Jia, Cui Zhang & Liang Wang

  2. The Fourth Department of Endocrinology, Cangzhou Central Hospital, Cangzhou, 061000, Hebei, China

    Weina Gao

Authors
  1. Wei Li
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  2. Zhe Song
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  3. Nan Jia
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  4. Cui Zhang
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  5. Weina Gao
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  6. Liang Wang
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Contributions

Wei Li and Liang Wang contributed to the study conception and design. Material preparation, data collection, and analysis were performed by Wei Li, Zhe Song, Nan Jia, and Cui Zhang. The first draft of the manuscript was written by Wei Li. Weina Gao and Liang Wang contributed reagents, materials, and analysis tools. All authors read and approved the final manuscript.

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Correspondence to Liang Wang.

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The authors declare they have no conflict of interest.

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Editor: Tetsuji Okamoto

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Li, W., Song, Z., Jia, N. et al. microRNA-4429-5p suppresses the malignant development of colon cancer by targeting matrix metalloproteinase 16. In Vitro Cell.Dev.Biol.-Animal 57, 715–725 (2021). https://doi.org/10.1007/s11626-021-00603-4

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  • DOI: https://doi.org/10.1007/s11626-021-00603-4

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