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Methyl viologen induces neural differentiation on murine P19 cells

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Abstract

Methyl viologen is a highly effective contact herbicide, a neurotoxic compound and an inducer of reactive oxygen species, which generate oxidative stress in cells. Reactive oxygen species has been known to function as an important messenger in cell differentiation. In this study, we investigated the effect of methyl viologen on neural cell differentiation using pluripotent mouse embryonal carcinoma P19 cells, which reportedly differentiate into neural cells upon exposure to retinoic acid. Methyl viologen, an inducer of intracellular reactive oxygen species, induced the differentiation of P19 cells into neural cells in the presence of neurofilament. Reduced glutathione, an eliminator of reactive oxygen species, also induced neural differentiation in P19 cells. These results suggest that P19 cells differentiate into neural cells, conceivably independent of intracellular reactive oxygen species.

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Acknowledgments

We are grateful to Koji Ogawa for his technical advice in cell culture.

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Authors and Affiliations

  1. Marine Bioresource Exploration Research Group, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15 Natsushima-cho, Yokosuka, Kanagawa, 237-0061, Japan

    Eriko Shimada, Tomohisa Ogawa, Kenta Tsutsui & Yusuke Tsuruwaka

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  1. Eriko Shimada
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  2. Tomohisa Ogawa
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  3. Kenta Tsutsui
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  4. Yusuke Tsuruwaka
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Correspondence to Yusuke Tsuruwaka.

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Editor: Tetsuji Okamoto

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Shimada, E., Ogawa, T., Tsutsui, K. et al. Methyl viologen induces neural differentiation on murine P19 cells. In Vitro Cell.Dev.Biol.-Animal 52, 466–472 (2016). https://doi.org/10.1007/s11626-016-0001-9

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  • DOI: https://doi.org/10.1007/s11626-016-0001-9

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