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Vitrification of Rattus norvegicus immature cumulus-oocyte complexes using hyaluronic acid

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Abstract

The aim of this study was to assess in vitro meiosis resumption and nuclear maturation of Rattus norvegicus oocytes after vitrification with different cryoprotective solutions. Cumulus-oocyte complexes (COCs) were exposed to an equilibration solution for 4 min placed in cryoprotective solutions for 1 min and vitrified in open pulled straws. Cryoprotective solutions were prepared with 15% ethylene glycol + 15% dimethyl sulfoxide + 0.5 M sucrose and different supplements, to form the following groups: G1, 20% fetal bovine serum in modified phosphate-buffered saline (mPBS); G2, 0.4% bovine serum albumine in mPBS; G3, 1% hyaluronic acid in mPBS; and G4, 0.4% polyvinyl alcohol in mPBS. Seven days after vitrification, the COCs from G1 to G4 were warmed and in vitro matured for 30 h along with the control group. Hoechst staining was performed to assess meiosis resumption and nuclear maturation rates. Control group showed higher meiosis resumption (77.88%) and nuclear maturation rates (55.75%) compared to all vitrified groups. Among the vitrified COCs, G3 showed the highest meiosis resumption and nuclear maturation rates (G1, 26.5 and 15.38%; G2, 22.12 and 11.54%; G3, 34.55 and 20%; G4, 20.17 and 9.24%). Supplementation of the vitrification solution with 1% hyaluronic acid provided better results, compared to the other supplements. Hyaluronic acid can be useful to vitrify rat COCs associated with other cryoprotectant agents.

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Authors and Affiliations

  1. Laboratório de Biologia Celular, Universidade Federal de Ciências da Saúde de Porto Alegre - UFCSPA, Rua Sarmento Leite, 245, Rio Grande do Sul, 90050-170, Brazil

    L. M. G. Paim, L. L. Gal & A. T. D. Oliveira

  2. Laboratório de Biotecnologia Animal Aplicada, Universidade Federal do Rio Grande do Sul (UFRGS), Rua Sarmento Leite, 500 - sala 001 D, Porto Alegre, Rio Grande do Sul, 90050-170, Brazil

    R. F. F. Lopes & A. T. D. Oliveira

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  1. L. M. G. Paim
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  2. L. L. Gal
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  4. A. T. D. Oliveira
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Correspondence to A. T. D. Oliveira.

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Paim, L.M.G., Gal, L.L., Lopes, R.F.F. et al. Vitrification of Rattus norvegicus immature cumulus-oocyte complexes using hyaluronic acid. In Vitro Cell.Dev.Biol.-Animal 51, 995–1002 (2015). https://doi.org/10.1007/s11626-015-9940-9

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