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Low-temperature Plasma Promotes Fibroblast Proliferation in Wound Healing by ROS-activated NF-κB Signaling Pathway

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Abstract

Low-temperature plasma (LTP) has shown great promise in wound healing, although the underlying mechanism remains poorly understood. In the present study, an argon atmospheric pressure plasma jet was employed to treat L929 murine fibroblasts cultured in vitro and skin wounds in BALB/c mice. The in vitro analysis revealed that treatment of fibroblasts with LTP for 15 s resulted in a significant increase in cell proliferation, secretion of epidermal growth factor (EGF) and transforming growth factor-βi (TGF-βi), production of intracellular reactive oxygen species (ROS), and the percentage of cells in S phase, protein expression of phosphorylated p65 (P-p65) and cyclinD1, but a noted decrease in the protein expression of inhibitor kappa B (IκB). The in vitro experiments demonstrated that 30-s LTP treatment enhanced the number of fibroblasts and the ability of collagen synthesis, while 50-s treatment led to the opposite outcomes. These results suggested that LTP treatment promotes the fibroblast proliferation in wound healing by inducing the generation of ROS, upregulating the expression of P-p65, downregulating the expression of IκB, and activating the NF-κB signaling pathway and consequently altering cell cycle progression (increased DNA synthesis in S phage).

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Acknowledgements

The authors would like to thank Prof. Chen Huang at the School of Medicine, Xi'an Jiaotong University, for his excellent technical help. The authors are also grateful to Y. X. Li, Z. Y. Peng and Z. H. Zhang for their contribution to data collection.

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Authors and Affiliations

  1. Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education, School of Public Health, Xi’an Jiaotong University, Xi’an, 710061, China

    Xing-min Shi  (石兴民), Jin-ren Liu  (刘选仁), Yue-ming Wu  (吴月明), Ling-ge Gao  (高菱鸽) & Yang Yang  (杨 阳)

  2. State Key Laboratory of Electrical Insulation and Power Equipment, School of Electrical Engineering, Xi’an Jiaotong University, Xi’an, 710049, China

    Gui-min Xu  (许桂敏), Guan-jun Zhang  (张冠军), Zheng-shi Chang  (常正实) & Cong-wei Yao  (姚聪伟)

Authors
  1. Xing-min Shi  (石兴民)
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  2. Gui-min Xu  (许桂敏)
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  3. Guan-jun Zhang  (张冠军)
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  4. Jin-ren Liu  (刘选仁)
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  5. Yue-ming Wu  (吴月明)
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  6. Ling-ge Gao  (高菱鸽)
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  7. Yang Yang  (杨 阳)
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  8. Zheng-shi Chang  (常正实)
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  9. Cong-wei Yao  (姚聪伟)
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Corresponding author

Correspondence to Guan-jun Zhang  (张冠军).

Additional information

"This project was supported by the National Natural Science Foundation of China (Nos. 81372076, 51677146, 51521065 and 51307133), and the Sci-Tech Project of Shaanxi Province (No. 2010K16-04).

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Shi, Xm., Xu, Gm., Zhang, Gj. et al. Low-temperature Plasma Promotes Fibroblast Proliferation in Wound Healing by ROS-activated NF-κB Signaling Pathway. CURR MED SCI 38, 107–114 (2018). https://doi.org/10.1007/s11596-018-1853-x

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  • DOI: https://doi.org/10.1007/s11596-018-1853-x

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