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The effect of brain-derived neurotrophic factor on angiogenesis

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Summary

To investigate the in vitro and in vivo proangiogenic effects of brain-derived neurotrophic factor (BDNF), human umbilical vein endothelial cells (HUVECs) were isolated and cultured in primary culture. The effect of BDNF on the proliferation of HUVECs was examined by MTT assay. The effects of BDNF on HUVEC migration and tube formation were studied by modified Boyden chamber assay and tube formation assay, respectively. Matrigel plug assay and chorioallantoic membrane assay were used to evaluate the effects of BDNF on angiogenesis in vivo. Our results showed that BDNF substantially stimulated the migration and tube formation of HUVECs in vitro, although it did not induce HUVEC proliferation. BDNF also induced angiogenesis both in matrigel plug of mouse model and in chick chorioallantoic membrane. In conclusion, BDNF can promote angiogenesis both in vitro and in vivo, and may be a proangiogenic factor.

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Authors and Affiliations

  1. Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China

    Chunyan Sun  (孙春艳), Yu Hu  (胡 豫), Zhangbo Chu  (褚章波), Jing Huang  (黄 靖) & Lu Zhang  (张 璐)

Authors
  1. Chunyan Sun  (孙春艳)
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  2. Yu Hu  (胡 豫)
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  3. Zhangbo Chu  (褚章波)
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  4. Jing Huang  (黄 靖)
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  5. Lu Zhang  (张 璐)
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Additional information

The project was supported by grants from the National Natural Sciences Foundation of China (No. 30670896), Youth Talent Foundation of Hubei Province (No. 2003 ABB017) and Scientific Research Foundation of Health Bureau of Hubei Province (No. JX3B06).

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Sun, C., Hu, Y., Chu, Z. et al. The effect of brain-derived neurotrophic factor on angiogenesis. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 29, 139–143 (2009). https://doi.org/10.1007/s11596-009-0201-6

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  • DOI: https://doi.org/10.1007/s11596-009-0201-6

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