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Hyaluronic Acid-RGD Peptide Conjugated Mesoporous Silica-coated Gold Nanorods for Cancer Dual-targeted Chemo-photothermal Therapy

  • Biomaterials
  • Published:
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Abstract

A multifunctional drug delivery system (GNRs@mSiO2-HA-RGD) was developed by conjugating targeting ligand hyaluronic acid (HA) and RGD with mesoporous silica- coated gold nanorods (GNRs@mSiO2) for dual-targeted chemo-photothermal therapy. The physiochemical properties of the prepared nanoparticles were characterized by FTIR, UV-vis spectra, and 1H NMR. Doxorubicin hydrochloride (DOX), an anticancer drug, was used as the model drug to investigate the drug loading, in vitro drug release profiles and cytotoxicity. The experimental results show that DOX-GNRs@mSiO2-HA-RGD is synthesized with a mean diameter of 116 nm and a sufficient load capacity of about 19.8%. It also has pH-enzyme sensitive and NIRtriggered drug release manner. Cellular uptake indicates that DOX-GNRs@mSiO2-HA-RGD exhibits a higher cellular uptake via CD44 receptor and integrin receptor mediated endocytosis compared with the GNRs@ mSiO2 modified with one receptor or no receptor. In comparison with chemotherapy or photothermal therapy alone, DOX-GNRs@mSiO2-HA-RGD displayes the synergistic effects and achieves a higher therapeutic efficacy. It can be expected that DOX-GNRs@mSiO2-HA-RGD is a potential dual-targeted chemo-photothermal therapeutic platform for effective cancer treatment.

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Author information

Author notes

  1. ZHOU Huimin and GAO Yuxiang are co-first authors, they contributed equally to the work.

Authors and Affiliations

  1. School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan, 430070, China

    Huimin Zhou  (周汇敏), Haixing Xu  (徐海星), Xin Li, Yahui Lü, Tian Ma, Xinjie Cai, Rui Li & Peihu Xu  (许沛虎)

  2. School of Materials and Science Engineering, Wuhan University of Technology, Wuhan, 430070, China

    Yuxiang Gao  (高玉香)

  3. Wuhan Kanghua Century Pharmaceutical Company, Wuhan, 430070, China

    Xiaobing Wang

Authors
  1. Huimin Zhou  (周汇敏)
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  2. Yuxiang Gao  (高玉香)
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  3. Haixing Xu  (徐海星)
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  4. Xin Li
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  5. Yahui Lü
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  6. Tian Ma
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  7. Xinjie Cai
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  8. Rui Li
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  9. Xiaobing Wang
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  10. Peihu Xu  (许沛虎)
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Corresponding authors

Correspondence to Haixing Xu  (徐海星) or Peihu Xu  (许沛虎).

Additional information

Funded by the National Natural Science Foundation of China (Nos. 51473130 and 51572206), the Wuhan Huanghe excellence plan and Entrepreneurship Training Program of Wuhan University and Technology (Nos. 20171049720018, 20171049720019, and 20171049720009)

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Zhou, H., Gao, Y., Xu, H. et al. Hyaluronic Acid-RGD Peptide Conjugated Mesoporous Silica-coated Gold Nanorods for Cancer Dual-targeted Chemo-photothermal Therapy. J. Wuhan Univ. Technol.-Mat. Sci. Edit. 33, 512–523 (2018). https://doi.org/10.1007/s11595-018-1853-4

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  • DOI: https://doi.org/10.1007/s11595-018-1853-4

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