Abstract
In this study, we isolated and identified oomycete species of the genus Saprolegnia. Saprolegnia atlantica, a new species isolated from soil and freshwater bodies in areas of the Atlantic Rainforest (São Paulo state, Brazil), is described herein based on morphology and phylogenetic analyses of combined ITS and LSU regions of the nuclear ribosomal DNA (nc rDNA). Taking into account the oospore type and/or the presence of terminal oogonia with androgynous antheridia, this new species appears to be related to S. litoralis and S. terrestris, but the phylogenetic placement of the isolates did not corroborate this relationship. Saprolegnia aenigmatica, S. blelhamensis, S. glomerata, and S. milanezii were also identified in the soil and submerged leaves, surface sediment, and/or water samples from freshwater bodies collected for the study. These specimens were sequenced, included in phylogenetic analyses, and described, commented and/or illustrated. Saprolegnia glomerata is a first report for Brazil, first sequences of S. blelhamensis and S. glomerata were deposited in GenBank, and Brazilian isolates of S. blelhamensis and S. glomerata are designated as epitypes. Our phylogenetic analyses of the Saprolegnia clade and previously published studies suggest that S. bulbosa, S. longicaulis, S. oliviae, and S. maragheica are new synonyms of S. ferax, and S. semihypogyna is a new synonym of S. eccentrica.
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The full text of the manuscript will be available in Mycologycal Progress, the sequences on GenBank and the specimens at the Herbarium SP and CCIBt Culture Collection.
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Acknowledgements
We thank “Instituto Florestal” for the permission given to collect samples in the studied areas and Manoel Osorio Neves Junior and Otto Hartung for their valuable help. The authors would also like to thank the English review by Robert Ryan Geyer and the valuable contributions of Dr. Bart Buyck.
Funding
C.L.A. Pires-Zottarelli (Process No. 2012/50222-7 and 2016/11146-4), D.R.S. Colombo (Process No. 2018/05602-2), and S.C.O. Rocha (Process No. 2014/04375-1) received grants from the FAPESP (“Fundação de Amparo à Pesquisa do Estado de São Paulo”). C.L.A. Pires-Zottarelli (Process No. 304091/2018-9) also received grants from the CNPq (“Conselho Nacional de Desenvolvimento Científico e Tecnológico”).
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All authors contributed to the conception and design of this study. Sampling and laboratory analysis were performed by Carmen Lidia Amorim Pires Zottarelli, Ana Lucia de Jesus, Sarah Cristina de Oliveira da Paixão, Marcela Castilho Boro, and Débora Rodrigues da Silva Colombo; phylogenetic reconstruction was prepared and interpreted by Ana Lucia de Jesus, Carmen Lidia Amorim Pires Zottarelli, and Sarah Cristina de Oliveira da Paixão; the first draft of the manuscript was written by Carmen Lidia Amorim Pires Zottarelli, and all authors commented on this version; review and editing were performed by Carmen Lidia Amorim Pires Zottarelli, Ana Lucia de Jesus, Sarah Cristina de Oliveira da Paixão, Marcela Castilho Boro, and Débora Rodrigues da Silva Colombo; funding acquisition by Carmen Lidia Amorim Pires Zottarelli as responsible. All authors read and approved the final version of the manuscript.
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Pires-Zottarelli, C.L.A., de Oliveira Da Paixão, S.C., da Silva Colombo, D.R. et al. Saprolegnia atlantica sp. nov. (Oomycota, Saprolegniaceae) from Brazil, and new synonymizations and epitypifications in the genus Saprolegnia. Mycol Progress 21, 41 (2022). https://doi.org/10.1007/s11557-022-01784-2
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DOI: https://doi.org/10.1007/s11557-022-01784-2