Abstract
An investigation of endophytic fungi on healthy leaves of Sorghum bicolor in Brazil led to the identification of an interesting fungus. Based on morphological features and multi-locus analyses, including ITS and LSU nrDNA, rpb1, and tef1 sequences, we propose a new genus, Bifusisporella, in the family Magnaporthaceae. The isolates exhibited a phialidic asexual morph with the following characteristics: curved conidiogenous cells, elongated, cylindrical or clavate, solitary or aggregate. Dimorphic conidia: macroconidia curved, falcate, hyaline, smooth, non-septate, guttulate, tapering at both ends; microconidia falcate, straight to slightly curved, hyaline, smooth, non-septate, hyphopodia are brown, smooth, elongated, and multi-lobulate.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Araújo WL, Lima AOS, Azevedo JL, Marcon J, Sobral JLP (2002) Manual: isolamento de microrganismos endofíticos. ESALQ, Piracicaba, p 86
Bussaban B, Lumyong S, Lumyong P, Hyde KD, McKenzie EHC (2001) Two new species of endophytes (Ascomycetes) from Zingiberaceae sporulating in culture. Nova Hedwigia 73:487–493. https://doi.org/10.1127/nova.hedwigia/73/2001/487
Cannon PF (1994) The newly recognized family Magnaporthaceae and its interrelationships. Syst Ascomycetum 13:25–42
Cannon PF, Alcorn JL (1994) Omnidemptus affinis gen. et sp. nov., teleomorph of Mycoleptodiscus affinis sp. nov. Mycotaxon 51:483–487
Carbone I, Kohn LM (1999) A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia 91:553–556. https://doi.org/10.2307/3761358
Castlebury L, Rossman A, Sung G, Hyten A, Spatafora J (2004) Multigene phylogeny reveals new lineage for Stachybotrys chartarum, the indoor air fungus. Mycol Res 108:864–872. https://doi.org/10.1017/S0953756204000607
Crous PW, Wingfield MJ, Guarro J, Hernández-Restrepo M, Sutton DA, Acharya K, Barber PA, Boekhout T, Dimitrov RA, Dueñas M, Dutta AK, Gené J, Gouliamova DE, Groenewald M, Lombard L, Morozova OV, Sarkar J et al (2015) Fungal Planet description sheets: 320–370. Persoonia 34:167–266. https://doi.org/10.3767/003158515X688433
Crous PW, Wingfield MJ, Burgess TI, Carnegie AJ, Hardy GESJ, Smith D, Summerell BA, Cano-Lira JF, Guarro J, Houbraken J, Lombard L, Martín MP, Sandoval-Denis M, Alexandrova AV et al (2017) Fungal Planet description sheets: 625–715. Persoonia 39:270–467. https://doi.org/10.3767/persoonia.2017.39.11
Griffiths RI, Whiteley AS, O’donnell AG, Bailey MJ (2000) Rapid method for coextraction of DNA and RNA from natural environments for analysis of ribosomal DNA and RRNA-based microbial community composition. Appl Environ Microbiol 66:5488–5491. https://doi.org/10.1128/AEM.66.12.5488-5491.2000
Hernández-Restrepo M, Groenewald JZ, Elliott ML, Canning G, McMillan VE, Crous PW (2016) Take-all or nothing. Stud Mycol 83:19–48. https://doi.org/10.1016/j.simyco.2016.06.002
Hornby D, Slope DB, Gutteridge RJ, Sivanesan A (1977) Gaeumannomyces cylindrosporus, a new ascomycete from cereal roots. Trans Br Mycol Soc 69:21–25. https://doi.org/10.1016/S0007-1536(77)80110-1
Katoh K, Standley DM (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Mol Biol Evol 30:772–780. https://doi.org/10.1093/molbev/mst010
Klaubauf S, Tharreau D, Fournier E, Groenewald JZ, Crous PW, De Vries RP, Lebrun MH (2014) Resolving the polyphyletic nature of Pyricularia (Pyriculariaceae). Stud Mycol 79:85–120. https://doi.org/10.1016/j.simyco.2014.09.004
Kohlmeyer J, Volkmann-Kohlmeyer B (1995) Fungi on Juncus roemerianus. I. Trichocladium medullare sp. nov. Mycotaxon 53:349–353
Kumar S, Stecher G, Tamura K (2016) MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874. https://doi.org/10.1093/molbev/msw054
Liu JK, Hyde KD, Jones EBG, Ariyawansa HA, Bhat DJ, Boonmee S, Maharachchikumbura SSN, McKenzie EHC, Phookamsak R, Phukhamsakda C et al (2015) Fungal diversity notes 1–110: taxonomic and phylogenetic contributions to fungal species. Fungal Divers 72:1–197. https://doi.org/10.1007/s13225-015-0324-y
Luo J, Zhang N (2013) Magnaporthiopsis, a new genus in Magnaporthaceae (Ascomycota). Mycologia 105:1019–1029. https://doi.org/10.3852/12-359
Luo J, Yin J, Cai L, Zhang K, Hyde KD (2004) Freshwater fungi in Lake Dianchi, a heavily polluted lake in Yunnan, China. Fungal Divers 16:93–112
Luo J, Walsh E, Zhang N (2014) Four new species in Magnaporthaceae from grass roots in New Jersey pine barrens. Mycologia 106:580–588. https://doi.org/10.3852/13-306
Luo J, Walsh E, Zhang N (2015) Toward monophyletic generic concepts in Magnaporthales: species with Harpophora asexual states. Mycologia 107:641–646. https://doi.org/10.3852/14-302
Luo J, Vines PL, Grimshaw A, Hoffman L, Walsh E, Bonos SA, Clarke B, Murphy JA, Meyer WA, Zhang N (2017) Magnaporthiopsis meyeri-festucae, sp. nov., associated with a summer patch-like disease of fine fescue turfgrasses. Mycologia 109:780–789. https://doi.org/10.1080/00275514.2017.1400306
Ma J, Xia JW, Castañeda-Ruíz RF, Zhang XG (2014) Nakataea setulosa sp. nov. and Uberispora formosa sp. nov. from southern China. Mycol Progress 13:753–758. https://doi.org/10.1007/s11557-013-0958-7
Matheny PB, Liu YJ, Ammirati JF, Hall BD (2002) Using RPB1 sequences to improve phylogenetic inference among mushrooms (Inocybe, Agaricales). Am J Bot 89:688–698. https://doi.org/10.3732/ajb.89.4.688
Nylander JAA (2004) MrModeltest 2.2. Computer program and documentation distributed by the author. Evolutionary Biology Centre, Uppsala University
Oliveira RJV, Lima TEF, Cunha IB, Coimbra VRM, Silva GA, Bezerra JL, Cavalcanti MAQ (2014) Corniculariella brasiliensis, a new species of coelomycetes in the rhizosphere of Caesalpinia echinata (Fabaceae, Caesalpinioideae) in Brazil. Phytotaxa 178:197–204. https://doi.org/10.11646/phytotaxa.178.3.5
Rayner RW (1970) A mycological colour chart. CMI and British Mycological Society, Kew
Rehner SA, Buckley E (2005) A Beauveria phylogeny inferred from nuclear ITS and EF1-a sequences: evidence for cryptic diversification and links to Cordyceps teleomorphs. Mycologia 97:84–98. https://doi.org/10.1080/15572536.2006.11832842
Shearer CA (1989) Pseudohalonectria (Lasiosphaeriaceae), an antagonistic genus from woodzin freshwater. Can J Bot 67:1944–1955. https://doi.org/10.1139/b89-247
Staden R, Beal KF, Bonfield JK (2000) The Staden package 1998. Methods Mol Biol 132:115–130. https://doi.org/10.1385/1-59259-192-2:115
Stamatakis A, Hoover P, Rougemont J (2008) A rapid bootstrap algorithm for the RAxML web servers. Syst Biol 57:758–771. https://doi.org/10.1080/10635150802429642
Thongkantha S, Jeewon R, Vijaykrishna D, Lumyong S, McKenzie EHC, Hyde KD (2009) Molecular phylogeny of Magnaporthaceae (Sordariomycetes) with a new species, Ophioceras chiangdaoensis from Dracaena loureiri in Thailand. Fungal Divers 34:157–173
Vilgalys R, Hester M (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. J Bacteriol 172:4238–4246. https://doi.org/10.1128/jb.172.8.4238-4246.1990
White TJ, Bruns T, Lee S, Taylor L (1990) Amplification and direct sequencing of fungal RNA genes for phylogenetics. In: Innis, Gelfand MA, Sninsky DH, White JJ, Eds TJ (eds) PCR protocols. A guide to methods and applications. Academic Press, pp 315–322
Wijayawardene NN, Hyde KD, Lumbsch HT, Liu JK, Maharachchikumbura SSN, Ekanayaka AH, Tian Q, Phookamsak R (2018) Outline of Ascomycota: 2017. Fungal Divers 88:167–263. https://doi.org/10.1007/s13225-018-0394-8
Yuan ZL, Lin FC, Zhang CL, Kubicek CP (2010) A new species of Harpophora (Magnaporthaceae) recovered from healthy wild rice (Oryza granulata) roots, representing a novel member of a beneficial dark septate endophyte. FEMS Microbiol Lett 7:94–101. https://doi.org/10.1111/j.1574-6968.2010.01963.x
Zhang N, Zhao S, Shen Q (2011) A six-gene phylogeny reveals the evolution of mode of infection in the rice blast fungus and allied species. Mycologia 103:1267–1276. https://doi.org/10.3852/11-022
Acknowledgments
The authors acknowledge the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES, Finance Code 001), the Fundação de Amparo à Ciência e Tecnologia de Pernambuco (FACEPE), and the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for providing fellowships (PQ Processes 312186/2016-9 and 312146/2014-0) and financial support to this research.
Author information
Authors and Affiliations
Corresponding author
Additional information
Responsible editor: Gerhard Rambold
Publisher’s note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Silva, R.M.F., Oliveira, R.J.V., Bezerra, J.D.P. et al. Bifusisporella sorghi gen. et sp. nov. (Magnaporthaceae) to accommodate an endophytic fungus from Brazil. Mycol Progress 18, 847–854 (2019). https://doi.org/10.1007/s11557-019-01494-2
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11557-019-01494-2