Abstract
In this study, pulsed laser ablation was utilized to produce gold nanoparticles (Au NPs), hydroxyapatite NPs, and then conjugated with cefuroxime as a heteronanocomposite structure targeting Klebsiella pneumoniae. When Klebsiella forms a biofilm, it shows a remarkable resistance against a variety of antibiotics. The heterostructure nanocomposite was characterized using UV/Vis spectrophotometry, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and transmission electron microscopy (TEM). The antibacterial activity of the heteronanocomposite against Klebsiella pneumoniae was assessed and compared with the cefuroxime alone. The inhibitory action of the heteronanocomposite of biofilm in the urinary catheter (Foley’s catheter) was examined by atomic force microscopy (AFM). The results of the current study demonstrated that the heteronanocomposite had antimicrobial activity greater than cefuroxime alone to stop the growth of microbial biofilms. This heteronanocomposite has shown a promising result, which could be a reliable therapy against Klebsiella pneumoniae in the future and could be a promising agent for succeeding bacterial biofilm inhibition during a urinary tract infection.
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The authors appreciated the University of Technology, Iraq, for the logistic support of this work.
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Hadeel M. Yosif and Majid S. Jabir: writing original draft, methodology, investigation and formal analysis. Buthenia A. Hasoon and Majid S. Jabir: main concept, data interpretation, and supervision. Majid S. Jabir and Buthenia A. Hasoon: writing—review and editing, visualization, and data curation. All authors reviewed the manuscript.
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Yosif, H.M., Hasoon, B.A. & Jabir, M.S. Laser Ablation for Synthesis of Hydroxyapatite and Au NP Conjugated Cefuroxime: Evaluation of Their Effects on the Biofilm Formation of Multidrug Resistance Klebsiella pneumoniae. Plasmonics 19, 1085–1099 (2024). https://doi.org/10.1007/s11468-023-02053-y
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DOI: https://doi.org/10.1007/s11468-023-02053-y