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Synergetic Effect of Memecylon edule Mediated AuNPs and Ultrasound Therapy as a Promising Approach Against Proliferation of Pediatric Acute Leukemia Cell Lines

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Abstract

In the present work, an easy one-pot environmentally friendly method is demonstrated where the extract of Memecylon edule was utilized for the synthesis of gold nanoparticles (Au NPs) through the reduction of chloroauric acid (HAuCl4). The synthesized NPs were studied using various spectroscopic and microscopic techniques. UV-visible (UV-Vis) results indicated the formation of AuNPs with optical absorption peak found at 537 nm. Transmission electron microscopy (TEM) and X-ray diffraction (XRD) analysis confirmed the formation of AuNPs with size ranges from 25 to 30 nm. FTIR results confirmed the capping of AuNPs with Memecylon edule extract polyphenols. Energy-dispersive spectroscopy (EDS) results confirmed the AuNPs with existence of elemental peaks corresponding to gold. Additionally, the present study also described the combined effect of bio-fabricated AuNPs with ultrasound treatment for higher destruction of cancerous cells. Flow cytometry results showed the effect of AuNPs and ultrasound on pediatric acute myeloid leukemia cell lines (Kasumi-1) and normal CMK cell lines, which exhibited a negligible reduction in the percentage of viable cells with combined treatment towards the normal CMK cells. Moreover, the percent of viable Kasumi-1 cells displayed a noteworthy decline for the combined treatment of AuNPs with US. Furthermore, these outcomes indicated that AuNP-aided US therapy can act as an active and unique approach for the target of cancer cells destruction.

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Data is available upon request from corresponding author.

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  1. Outpatient Department of Pediatrics, Children’s Hospital of Shanxi and Women Health Center of Shanxi, Taiyuan, 030000, Shanxi, China

    Huiqing Guo

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Huiqing Guo: performing experiments, analysis of data, manuscript writing, and all other complete works related to this research.

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Guo, H. Synergetic Effect of Memecylon edule Mediated AuNPs and Ultrasound Therapy as a Promising Approach Against Proliferation of Pediatric Acute Leukemia Cell Lines. Plasmonics 19, 925–932 (2024). https://doi.org/10.1007/s11468-023-02045-y

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